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Application of the Cryobank Concept to Wild and Endangered Carnivora Species

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Abstract

This work reviews current advances in cryobanking gametes and preimplantation embryos for members of the order Carnivora. Problems specific to the application of current reproductive technologies to vulnerable and endangered carnivoran species are also discussed. In particular, cryopreservation of Carnivora species’ oocytes and embryos is complicated by the abundance of lipid droplets in them. The review focuses on Felidae, Canidae, Mustelidae, and Ursidae because the most experience has been gained in these families in using the achievements of reproductive biology to preserve the genetic resources of nondomesticated species, including endangered ones. For comparison, studies on domesticated members of these families are reviewed: the cat, the dog, and the domestic ferret. Specific features of reproductive biological of the four families are taken into account.

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ACKNOWLEDGMENTS

We thank the Laboratory of Animals Genetic Resources Shared Use Center and the Biological Objects Microscopy Shared Use Center of the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences.

Funding

This work was supported by the Russian Foundation for Basic Research (project no. 20-34-90093) and the budget project of the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences (project no. AAAA-A19-119100290012-8).

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    S. Ya. Amstislavsky, V. I. Mokrousova, S. V. Okotrub, E. Yu. Brusentsev & V. A. Naprimerov

  2. Novosibirsk National Research State University, 630090, Novosibirsk, Russia

    S. V. Okotrub

  3. Novosibirsk State Agrarian University, 630037, Novosibirsk, Russia

    V. A. Naprimerov

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  1. S. Ya. Amstislavsky
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  2. V. I. Mokrousova
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  4. E. Yu. Brusentsev
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  5. V. A. Naprimerov
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Correspondence to S. Ya. Amstislavsky.

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The authors declare that they have no conflict of interests. This article does not contain any studies involving animals or human participants performed by any of the authors.

AUTHOR’S CONTRIBUTIONS

V.I. Mokrousova, E.Yu. Brusentsev, and S.Ya. Amsti-slavsky wrote the section “Cryopreservation of Spermatozoa of Representatives of the Order of Carnivores” and prepared the tables; S.V. Okotrub and V.A. Naprimerov wrote the section “Cryopreservation of Oocytes and Preimplantation Embryos of Representatives of the Order of Carnivores”; S.Ya. Amstislavsky edited the manuscript.

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Translated by M. Belenkii

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Amstislavsky, S.Y., Mokrousova, V.I., Okotrub, S.V. et al. Application of the Cryobank Concept to Wild and Endangered Carnivora Species. Russ J Dev Biol 52, 301–318 (2021). https://doi.org/10.1134/S1062360421040020

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  • DOI: https://doi.org/10.1134/S1062360421040020

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