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Detection Limits of Immunoanalytical Systems: Limiting Factors and Methods of Reduction

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Abstract

One of the priority directions in the development of modern immunoanalytical systems is a reliable determination of analytes in very low concentrations. In this regard, an assessment of the factors influencing the detection limit and restricting its further decrease is in great demand. This mini-review considers a variety of immunoanalytical systems and theoretical estimation of their detection limits. The possibilities of reducing the detection limit provided by changing the composition of the detected immune complexes, using new markers included in these complexes, various methods of their registration, and enhancing the recorded signal are characterized. The approaches proposed for the detection of single molecules of target analytes in tested samples are discussed.

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ACKNOWLEDGMENTS

This study was financially supported by the Russian Science Foundation (grant 19-14-00370). The authors are grateful to N.A. Taranova for preparing the illustrative material.

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  1. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia

    A. V. Zherdev & B. B. Dzantiev

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  1. A. V. Zherdev
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  2. B. B. Dzantiev
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Correspondence to B. B. Dzantiev.

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Zherdev, A.V., Dzantiev, B.B. Detection Limits of Immunoanalytical Systems: Limiting Factors and Methods of Reduction. J Anal Chem 77, 391–401 (2022). https://doi.org/10.1134/S1061934822040141

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  • DOI: https://doi.org/10.1134/S1061934822040141

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