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An electrochemical DNA sensor for determination of 6-thioguanine using adsorptive stripping voltammetry at HMDE: An anticancer drug DNA interaction study

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Abstract

In the present study, the electrochemical behavior of 6-Thioguanine (6-TG) and its interaction with double-strand DNA (ds-DNA) were investigated at the surface of hanging mercury drop electrode (HMDE) in neutral media. The interaction of 6-TG with ds-DNA in neutral buffer was clearly demonstrated by the elimination of 6-TG signal and the advent of a new reduction peak. To investigate the interaction, various parameters including accumulation time and potential as well as ds-DNA concentration were optimized using a combination of adsorptive stripping voltammetry (AdSV) and square wave voltammetry (SWV) techniques. As a consequence a low detection limit of 1.1 μM was obtained in a dynamic range of 16.0 to 360.0 μM. To better understand the interaction mechanism between 6-TG anti-cancer drug and ds-DNA, cyclic voltammetry and UV-Vis spectroscopy measurements were carried out and the intercalation of 6-TG into ds-DNA was proposed as the plausible mechanism. The application of this screening assay in real sample analysis was investigated by using the procedure for determination of 6-TG in 6-Thioguanine tablets and also in spiked 6-TG blood serum. Overall, the results were indicative of a DNA sensor which could be applied effectively in the analysis of 6-TG in vitro.

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Authors and Affiliations

  1. Department of Science, Islamic Azad University, Shahrekord Branch, Shahrelcord, 88137, Iran

    Elham Mirmomtaz

  2. Department of Chemistry, Islamic Azad University, Shahreza Branch, Shahreza, 86145, Iran

    Ali Zirakbash

  3. Department of Chemistry, Isfahan University of Technology, Isfahan, 84156, Iran

    Ali Asghar Ensafi

Authors
  1. Elham Mirmomtaz
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  2. Ali Zirakbash
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  3. Ali Asghar Ensafi
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Corresponding author

Correspondence to Elham Mirmomtaz.

Additional information

Published in Russian in Elektrokhimiya, 2016, Vol. 52, No. 4, pp. 369–378.

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Mirmomtaz, E., Zirakbash, A. & Ensafi, A.A. An electrochemical DNA sensor for determination of 6-thioguanine using adsorptive stripping voltammetry at HMDE: An anticancer drug DNA interaction study. Russ J Electrochem 52, 320–329 (2016). https://doi.org/10.1134/S1023193516040078

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  • DOI: https://doi.org/10.1134/S1023193516040078

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