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Polymorphism of DMRT3 Gene and Its Association with Body Measurements in Horse Breeds

  • ANIMAL GENETICS
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Abstract

The double sex and mab-3-related transcription factor 3 gene (DMRT3) placed on chromosome 23 of equine. This gene codes a major transcription factor in the spinal cord complex encompasses regulating motion of vertebrates. It is implicated in a neural network for the synchrony of the moving apparatus that governs limb motion. The aim of this study was to determine polymorphism of DMRT3 gene in Iranian exotic and native horse breeds (Persian Arab, Akhal teke, Yamut, Kurd, Dareshouri, Caspian, Taleshi) using of PCR-RFLP technique and its correlation with body measurements. DNA was extracted from blood samples of 374 horses and a 485-bp fragment within the horse DMRT3 gene was synthesized applying specific primers. The PCR products were digested with DdeI restriction enzyme at 37°C overnight, and the resulting products were separated by the 3.5% agarose gel and visualized by ethidium bromide staining. Body measurements and gene association analyses were performed using a statistical model containing the fixed effects of breed, sex and the animal genotype for the DMRT3 locus. In all of the studied populations two genotypes, CC and AC, were observed while AA was not observed. Frequencies of C and A alleles were 0.97 and 0.03%, respectively. The AC genotype was only observed in Iranian native breeds including Turkmen (Akhal teke), Turkmen (Yamut), Dareshouri, Kurd and Caspian, while the other 12 remained breeds had only CC genotype. Number of observed alleles, number of effective alleles, observed heterozygosity, expected heterozygosity, Shanon’s index, Nei’s index and fixation index (Fis) for all studied breeds were 2.00, 1.06, 0.06, 0.06, 0.14, 0.06 and 0.10, respectively. A UPGMA dendrogram based on the Nei’s standard genetic interval between studied breeds displayed distinct clusters for Iranian native and exotic breeds. Statistical association analysis of two observed genotypes with body measurements showed that the studied mutation has no effect on body measurements.

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REFERENCES

  1. Fotovati, A., Persian horse breeds from ancient time to present and their rules in development of world horse breeds, Asian-Australas. J. Anim. Sci., 2000, vol. 13, no. 1, pp. 401—410.

    Article  Google Scholar 

  2. Hendricks, B.L., International Encyclopedia of Horse Breeds, Oklahoma: University of Oklahoma Press, 1995, 1st ed.

    Google Scholar 

  3. Digard, J.P., Asb-Savārī, Encyclopædia Iranica, 2012, vol. 2, no. 7, pp. 737—739.

    Google Scholar 

  4. Khodabakhshzadeh, R., Mohammadabadi, M.R., Esmailizadeh, A.K., Shahrebabak, H.M., Bordbar, F., and Namin, S.A., Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep, Pol. J. Vet. Sci., 2016, vol. 19, no. 2, pp. 281—289.

    Article  CAS  Google Scholar 

  5. Zamani, P., Akhondi, M., and Mohammadabadi, M., Associations of inter-simple sequence repeat loci with predicted breeding values of body weight in sheep, Small Ruminant Res., 2015, vol. 132, no. 1, pp. 123—127.

    Article  Google Scholar 

  6. Ebrahimi, M.T., Mohammadabadi, M., and Esmailizadeh, A., Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran, Arch. Tierzucht, 2017, vol. 60, no. 3, pp. 183—189.

    Google Scholar 

  7. Shamsalddini, S., Mohammadabadi, M.R., and Esmailizadeh, A.K., Polymorphism of the prolactin gene and its effect on fiber traits in goat, Russ. J. Genet., 2016, vol. 52, no. 4, pp. 405—408.

    Article  CAS  Google Scholar 

  8. Mohammadabadi, M.R., Nikbakhti, M., Mirzaee, H.R., Shandi, A., Saghi, D.A., Romanov, M.N., and Moiseyeva, I.G., Genetic variability in three native Iranian chicken populations of the Khorasan province based on microsatellite markers, Russ. J. Genet., 2010, vol. 46, no. 4, 505—509.

    Article  CAS  Google Scholar 

  9. Ruzina, M.N., Shtyfurko, T.A., Mohammadabadi, M.R., Gendzhieva, O.B., Tsedev, T., and Sulimova, G.E., Polymorphism of the BoLA-DRB3 gene in the Mongolian, Kalmyk, and Yakut cattle breeds, Russ. J. Genet., 2010, vol. 46, no. 4, pp. 456—463.

    Article  CAS  Google Scholar 

  10. Alexander, R.M., Why mammals gallop, Am. Zool., 1988, vol. 28, no. 1, pp. 237—245.

    Article  Google Scholar 

  11. Clayton, H.M., The Dynamic Horse, Michigan: USA Sport Horse Publications, 2004.

    Google Scholar 

  12. Andersson, L.S., Larhammar, M., Memic, F., Wootz, H., Schwochow, D., Rubin, C.J., Patra, K., Arnason, T., Wellbring, L., Hjälm, G., and Imsland, F., Mutations in DMRT3 affect locomotion in horses and spinal circuit function in mice, Nature, 2012, vol. 488, no. 7413, pp. 642—646.

    Article  CAS  Google Scholar 

  13. Promerová, M., Andersson, L.S., Juras, R., Penedo, M.C.T., Reissmann, M., Tozaki, T., Bellone, R., Dunner, S., Horín, P., Imsland, F., Imsland, P., Mikko, S., Modrý, D., Roed, K.H., Schwochow, D., et al., Worldwide frequency distribution of the ‘Gait keeper’ mutation in the DMRT3 gene. Animal Genetics, 2014, vol. 45, no. 1, pp. 274—482.

    Article  Google Scholar 

  14. Regatieri, I.C., Pereira, G.L., Neto, A.R.T., Ferraz, G.C., Curi, R.A., and Queiroz-Neto, A., Polymorphisms in MCT1, CD147, PDK4, and DMRT3 genes in Arabian and Quarter Horses, J. Equine Vet. Sci., 2017, vol. 48, no. 1, pp. 161—165.

    Article  Google Scholar 

  15. Patterson, L., Staiger, E.A., and Brooks, S.A., DMRT3 is associated with gait type in Mangalarga Marchador horses, but does not control gait ability, Anim. Genet., 2015, vol. 46, no. 1, pp. 213—215.

    Article  CAS  Google Scholar 

  16. Sánchez, M.J., Gómez, M.D., Peña, F., Monterde, J.G., Morales, J.L., Molina, A., and Valera, M., Relationship between conformation traits and gait characteristics in Pura Raza Español horses, Arch. Tierzucht, 2013, vol. 56, no. 13, pp. 137—148.

  17. Kristjánsson, T., Riding ability in Icelandic horses—effect of conformation and the ‘Gait keeper’ mutation in the DMRT3 gene, Doctoral thesis, Agricultural University of Iceland, Faculty of Land and Animal Resources, 2014.

  18. Signer-Hasler, H., Flury, C., Haase, B., Burger, D., Simianer, H., Leeb, T., and Rieder, S., A genome-wide association study reveals loci influencing height and other conformation traits in horses, PLoS One, 2012, vol. 7, no. 5, pp. 1—10.

    Article  Google Scholar 

  19. Han, H., Zeng, L., Dang, R., Lan, X., Chen, H., and Lei, C., The DMRT3 gene mutation in Chinese horse breeds, Stichting Int. Found. Anim. Genet., 2015, vol. 46, no. 3, pp. 340—342.

    Google Scholar 

  20. Yeh, F.C., Yang, R.C., and Boyle, T., POPGENE, Microsoft Windows Based Freeware For Population Genetic Analysis, Release 1.31, University of Alberta, 1999.

    Google Scholar 

  21. Gogel, A.R., Cullis, B.J., and Thompson, R., ASReml User Guide, Hemel Hempstead, UK: NSW Department of Industry and Investment, 2009.

    Google Scholar 

  22. Kristjansson, T., Bjornsdottir, S., Sigurdsson, A., Andersson, L.S., Lindgren, S.J., Klonowski, A.M., and Arnason, G.T., The effect of the ‘Gait keeper’ mutation in the DMRT3 gene on gaiting ability in Icelandic horses, J. Anim. Breed. Genet., 2014, vol. 131, no. 1, pp. 415—425.

    Article  CAS  Google Scholar 

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ACKNOWLEDGMENTS

We acknowledge the technical supports from the Research-Based Karmania Horse Company. Also, we wish to thank the enormous farmers who helped us in collecting the horse samples across the country.

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Authors and Affiliations

  1. Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

    I. Moazemi, M. R. Mohammadabadi, A. Mostafavi & A. K. Esmailizadeh

  2. Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine

    O. I. Babenko, M. V. Bushtruk, S. V. Tkachenko, R. V. Stavetska & N. I. Klopenko

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  1. I. Moazemi
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  2. M. R. Mohammadabadi
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  3. A. Mostafavi
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  4. A. K. Esmailizadeh
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  5. O. I. Babenko
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  6. M. V. Bushtruk
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  7. S. V. Tkachenko
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  8. R. V. Stavetska
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  9. N. I. Klopenko
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Correspondence to M. R. Mohammadabadi.

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Moazemi, I., Mohammadabadi, M.R., Mostafavi, A. et al. Polymorphism of DMRT3 Gene and Its Association with Body Measurements in Horse Breeds. Russ J Genet 56, 1232–1240 (2020). https://doi.org/10.1134/S1022795420100087

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  • DOI: https://doi.org/10.1134/S1022795420100087

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