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Measuring Genes Expression Involved in Enzymatic Defense and ABA Biosynthesis in Solanum lycopersicum L. (Red Cloud Cultivar) under Cold Stress

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Abstract

‘Red Cloud’ (RC) is a cultivar of tomato (Solanum lycopersicum L.) which one of the main agricultural products classified as cultivated plants, it is very important in many countries throughout the world. RC prefers open sunny areas. For this plant, temperature variations are important and have solutions to improve the effects of cold stress by many ways. This study was undertaken to determine the expression of FeSOD (iron superoxide dismutase) and SlNCED1 (a key enzyme in the ABA biosynthetic pathway) genes using qRT-PCR and some physiological features of RC under cold stress. For this purpose, after flowering and fruiting, the potted tomatoes were placed at 2, 4 and –1°C for 8, 12 and 24 h. To measure superoxide dismutase (SOD) enzyme activity, the extract of RC fruits was prepared, and the total protein content (TPrC) was analysed using the standard curve of Bovine Serum Albumin (BSA). Moreover, non-enzymatic antioxidants and total phenol contents (TPC) were measured by diphenyl-picrylhydrazyl (DPPH) and Folin Ciocalteu methods respectively. The results illustrated that all samples at 2°C and 12 h showed the lowest IC50 value, and also the highest amount of TPC was observed in 2–12 sample. Compared to the control, in all treatment groups, FeSOD and SlNCED1 genes expression were higher and much more significant at 4°C, 12 and 24 h, respectively. It has also been demonstrated that at lower temperatures, the TPrC raised and simultaneously, SOD showed the highest levels of activity.

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Authors and Affiliations

  1. Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

    N. Samadi, H. Abbaspour & N. Masoudian

  2. Department of Agriculture, Factually of Shariaty, Tehran Branch, Technical and Vocational University (TVU), Tehran, Iran

    S. Saeidi-Sar

Authors
  1. N. Samadi
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  2. S. Saeidi-Sar
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Correspondence to S. Saeidi-Sar.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as objects of research.

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Abbreviations: RC—‘Red Cloud’; TPC—total phenol content; TPrC—total protein content.

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Samadi, N., Saeidi-Sar, S., Abbaspour, H. et al. Measuring Genes Expression Involved in Enzymatic Defense and ABA Biosynthesis in Solanum lycopersicum L. (Red Cloud Cultivar) under Cold Stress. Russ J Plant Physiol 67, 131–138 (2020). https://doi.org/10.1134/S1021443720010173

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  • DOI: https://doi.org/10.1134/S1021443720010173

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