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Proteomic analysis of isozymes in Butea superba leaves

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Abstract

The proliferation of protein abundances under influence of seasonal changes are involved in cellular plant metabolic pathways. The protein profiles in relevant with seasonal variations in Butea superba leaves collected in winter, summer and rainy season were evaluated by two-dimensional polyacrylamide gel electrophoresis coupled with a nanoflow liquid chromatography coupled to electrospray ionization quadrupoletime- of-flight tandem mass spectrometry. The 84 proliferated protein spots were sum up of total proteins, which were found in 3 season-collected plant leaves and were classified into 11 functional groups. Eight proteins which exhibited the alteration of abundant levels in different seasons were found involved in carbohydrate and energy metabolism, photosynthesis, secondary metabolism, stress, RNA metabolism, ROS scavenging and detoxifying, and protein destination and storage. There were 2 proteins exhibited obviously isozyme polypeptide sequences. The variable physiological status within the plant leaf might be influenced by the quantitative and qualitative proliferated protein presences with response to seasonal changes and thus allowing plant to survive during severe abiotic stresses during seasonal changes.

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Abbreviations

2-DE:

two-dimensional polyacrylamide gel electrophoresis

ACN:

acetonitrile

CHAPS:

3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate

Vh:

volthours

MDHAR:

monodehydroascorbate peroxidase reductase

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Authors and Affiliations

  1. Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    C. Leelahawong

  2. Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, 10210, Thailand

    C. Srisomsap & D. Chokchaichamnankit

  3. Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    W. Cherdshewasart

  4. Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    N. Vinayavekhin & P. Sangvanich

Authors
  1. C. Leelahawong
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  2. C. Srisomsap
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  3. W. Cherdshewasart
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  4. D. Chokchaichamnankit
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  5. N. Vinayavekhin
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  6. P. Sangvanich
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Corresponding author

Correspondence to P. Sangvanich.

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Leelahawong, C., Srisomsap, C., Cherdshewasart, W. et al. Proteomic analysis of isozymes in Butea superba leaves. Russ J Plant Physiol 64, 100–108 (2017). https://doi.org/10.1134/S1021443717010101

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  • DOI: https://doi.org/10.1134/S1021443717010101

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