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Single-Stage Bioconversion of Phytosterol into Testosterone by Recombinant Strains of Mycolicibacterium neoaurum

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Abstract

A plasmid containing the genes encoding a fungal 17β-hydroxysteroid dehydrogenase, which ca-talyzes the reduction of 17-oxo group, and a mycobacterial glucose-6-phosphate dehydrogenase, which promotes the recycling of the essential coenzyme NAD(P)H, was constructed. Its constitutive expression in well-studied Mycolicibacterium neoaurum strains made it possible to increase the yield of C-17 hydroxysteroids significantly. In particular, recombinant strains created on the basis of M. neoaurum VKM Ac-1815D and M. neoaurum NRRL B-3805 ΔkstD exhibited predominant accumulation of testosterone, while the strain based on M. neoaurum VKM Ac-1816D accumulated 1(2)-dehydrotestosterone and testosterone simultaneously.

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Funding

The study was supported by the Russian Science Foundation, project no. 21-64-00024.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290, Pushchino, Russia

    D. N. Tekucheva, M. V. Karpov, V. V. Fokina, T. A. Timakova, A. A. Shutov & M. V. Donova

Authors
  1. D. N. Tekucheva
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  2. M. V. Karpov
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  3. V. V. Fokina
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  4. T. A. Timakova
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  5. A. A. Shutov
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  6. M. V. Donova
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Corresponding author

Correspondence to D. N. Tekucheva.

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Translated by E. Makeeva

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Tekucheva, D.N., Karpov, M.V., Fokina, V.V. et al. Single-Stage Bioconversion of Phytosterol into Testosterone by Recombinant Strains of Mycolicibacterium neoaurum. Microbiology 93, 134–138 (2024). https://doi.org/10.1134/S0026261723603913

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  • DOI: https://doi.org/10.1134/S0026261723603913

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