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Effect of Eu3+ on Calcium-Dependent Processes in Vertebrate Myocardium

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Abstract

The inotropic and chronotropic effects of Eu3+ on spontaneous atrial contraction of the frog Rana ridibunda myocardium at different temperatures were studied, as well as the effects of Ca2+ and Eu3+ on isolated rat heart mitochondria, energized with glutamate and malate (the Ist respiratory complex substrates). It turned out that Eu3+ in Ringer’s solution significantly reduced the amplitude of spontaneous contractions. However, the half-relaxation period of spontaneous contractions changed insignificantly. At the same time, Eu3+ also revealed a negative chronotropic effect, which is manifested in a decrease in the frequency of spontaneous contractions. The mitochondrial swelling study showed that Eu3+ increased the energy-dependent K+ transport into the matrix in the K-acetate medium. However, Eu3+ did not affect the inner membrane proton permeability since the mitochondria swelling in the medium with NH4NO3 did not depend on the presence of Eu3+. State 40 mitochondrial respiration (substrate in the medium) was slightly increased by Eu3+. At the same time, Eu3+ did not affect the energized mitochondria respiration in the presence of ADP (state 3) or 2,4-dinitrophenol (state 3UDNP). The respiration decreased in experiments with calcium load of mitochondria regardless of the medium presence of Eu3+. Eu3+ diminished the mitochondria Ca2+-induced swelling in the above media experiments. This result can be associated with the opening of the mitochondrial permeability transition pore in their inner membrane. Eu3+ did not affect the inner membrane potential (ΔΨmito). The data obtained allow us to understand better the Eu3+ action mechanisms on Ca2+-dependent processes in the vertebrate myocardium and mitochondria.

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ACKNOWLEDGMENTS

The authors are grateful to I.V. Brailovskaya for her help in isolating mitochondria and polarographic measurements of oxygen uptake rates by these organelles. Studies to determine the mitochondrial potential were carried out on the basis of the Center of collective usage in the IEPhB RAS.

Funding

The work was performed with the use of state budget funds under the state task no. 075-0152-22-00.

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  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    S. M. Korotkov, K. V. Sobol, A. V. Novozhilov & V. P. Nesterov

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Contributions

S.M.K. performed planning, data collection and processing, writing and editing of the article, and investigated mitochondrial swelling. C.V.S. investigated the contractile characteristics of cardiac muscle preparations of the frog Rana ridibunda and participated in the writing of the corresponding experimental sections of the manuscript, as well as in the discussion of this work. A.V.N. investigated the inner membrane potential of energized RHM. V.P.N. participated in the introduction and discussion of the results of the article.

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Correspondence to S. M. Korotkov.

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Translated by A. Dyomina

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Korotkov, S.M., Sobol, K.V., Novozhilov, A.V. et al. Effect of Eu3+ on Calcium-Dependent Processes in Vertebrate Myocardium. J Evol Biochem Phys 58 (Suppl 1), S52–S62 (2022). https://doi.org/10.1134/S0022093022070067

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