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Influence of Chronic Lead Intoxication on Functional Characteristics and Isoform Composition of Left Ventricular Myosin in the Rat Heart

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Abstract

The chronic lead intoxication was modelled by injecting outbred rats intraperitoneally with lead acetate 3 times a week for 5 weeks. Using an in vitro motility assay, it was shown that lead intoxication causes changes in in vitro actin–myosin interaction, specifically, a decrease in the maximum sliding velocity of native thin filaments over myosin isolated from the left ventricular myocardium of the rat heart. No statistically significant changes were found in the calcium sensitivity and cooperativity of the “pCa–velocity” curve, characteristics of the motile filament fraction, and isometric force. Using electrophoretic separation of proteins, a shift in the ratio of myosin heavy chains toward β-chains with a lower ATPase activity was found.

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Funding

This work was implemented within the Governmental assignment to the Institute of Immunology and Physiology (Ural Branch of the Russian Academy of Sciences, Yekaterinburg); theme reg. Nos. АААА-А19-119070190064-4 and AAAA–A18–118020590135–3 on the institutional equipment of the Center for Collective Use.

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  1. Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russia

    O. P. Gerzen, S. R. Nabiev & L. V. Nikitina

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  1. O. P. Gerzen
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  2. S. R. Nabiev
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  3. L. V. Nikitina
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Contributions

Idea and design of the experiment: L.V.N. and O.P.G.; data collection and processing: O.P.G. and S.R.N.; manuscript writing and editing: O.P.G. and L.V.N.

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Correspondence to O. P. Gerzen.

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The authors declare that they have neither evident nor potential conflict of interest associated with the publication of this article.

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Translated by A. Polyanovsky

Russian Text © The Author(s), 2021, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2021, Vol. 107, Nos. 6–7, pp. 854–863https://doi.org/10.31857/S0869813921060030.

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Gerzen, O.P., Nabiev, S.R. & Nikitina, L.V. Influence of Chronic Lead Intoxication on Functional Characteristics and Isoform Composition of Left Ventricular Myosin in the Rat Heart. J Evol Biochem Phys 57, 896–903 (2021). https://doi.org/10.1134/S002209302104013X

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