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The Effect of Experimental Hyperthyroidism on Characteristics of Actin–Myosin Interaction in Fast and Slow Skeletal Muscles

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Abstract

The molecular mechanism of the failure of contractile function of skeletal muscles caused by oxidative damage to myosin in hyperthyroidism is not fully understood. Using an in vitro motility assay, we studied the effect of myosin damage caused by oxidative stress in experimental hyperthyroidism on the actin–myosin interaction and its regulation by calcium. We found that hyperthyroidism-induced oxidation of myosin is accompanied by a decrease in the sliding velocity of the regulated thin filaments in the in vitro motility assay, and this effect is increased with the duration of the pathological process.

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Abbreviations

FT3:

free triiodothyronine

FT4:

free thyroxine

h :

Hill cooperativity coefficient

MHC:

myosin heavy chains

MLC:

myosin light chains

pCa:

negative decimal logarithm of the calcium concentration

pCa50 :

calcium concentration at which half-maximal sliding velocity of thin filaments is achieved (the calcium sensitivity)

V max :

the maximal sliding velocity of thin filaments

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Authors and Affiliations

  1. Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences, 620049, Yekaterinburg, Russia

    G. V. Kopylova, D. V. Shchepkin & S. Y. Bershitsky

Authors
  1. G. V. Kopylova
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  2. D. V. Shchepkin
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  3. S. Y. Bershitsky
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Correspondence to G. V. Kopylova.

Additional information

Published in Russian in Biokhimiya, 2018, Vol. 83, No. 5, pp. 695–702.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM17-426, March 5, 2018.

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Kopylova, G.V., Shchepkin, D.V. & Bershitsky, S.Y. The Effect of Experimental Hyperthyroidism on Characteristics of Actin–Myosin Interaction in Fast and Slow Skeletal Muscles. Biochemistry Moscow 83, 527–533 (2018). https://doi.org/10.1134/S000629791805005X

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  • DOI: https://doi.org/10.1134/S000629791805005X

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