Abstract
Photophysical characteristics and photosensitizing activity were studied for dimethyl (DME) and trimethyl ester (TME) derivatives of chlorin e6 (Chl e6) in various solutions and liposomal forms. Incorporation in liposomes rendered the Chl e6 ester derivatives monomeric in aqueous solutions keeping intact photophysical properties and high photochemical activity. The redistribution rate of Chl e6 DME from lipid vesicles to cells was substantially higher than that of Chl e6 TME. Serum proteins affected the intracellular accumulation of liposomal forms for the DME and TME derivatives of Chl e6 differently. Cell-culture experiments showed that liposomal forms of the DME and TME derivatives of Chl e6 had significantly lower cytotoxicity, while preserving a high cytotoxic effect of photodynamic activity.
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Abbreviations
- Chl e6 :
-
chlorin e6;
- DME:
-
dimethyl ester
- TME:
-
trimethyl ester
- DMPC:
-
dimyristoylphosphatidylcholine
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Original Russian Text © T.E. Zorina, I.V. Yankovsky, I.E. Kravchenko, T.V. Shman, M.V. Belevtsev, V.P. Zorin, 2015, published in Biofizika, 2015, Vol. 60, No. 5, pp. 922–930.
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Zorina, T.E., Yankovsky, I.V., Kravchenko, I.E. et al. Evaluation of Phototoxicity and cytotoxicity for chlorin e6 ester derivatives and their liposomal forms. BIOPHYSICS 60, 759–766 (2015). https://doi.org/10.1134/S0006350915050267
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DOI: https://doi.org/10.1134/S0006350915050267