Skip to main content
SpringerLink
Log in

Antifungal Activity of Chitosan Against Rhizoctonia solani f.sp. sasakii

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

Repeated in vitro and in vivo trials were conducted to evaluate antifungal activity of chitosan against Rhizoctonia solani f.sp. sasakii (RSS) causal organism for banded leaf and sheath blight of maize. Different concentrations of chitosan (viz., 0.2, 0.4 0.6, 0.8 and 1 mg/mL) were evaluated under controlled environment. Maximum growth inhibition (57.5%) was recorded with chitosan 1 mg/mL. Growth of RSS was slowed down with repeated sub-culturing of tested fungus in chitosan-amended medium indicating fungistatic effect of the chitosan. An increased chitosanase activity was recorded when chitosan was supplemented in the growth medium as sole carbon source compared to variant with dextrose after 15 days of incubation. Transmission electron microscopy showed that chitosan caused cellular disorganisation, increased number of vacuoles and reduced appearance of protoplasts as compared to non-chitosan treatment in the mycelia of RSS. Under in vivo trials, chitosan significantly (P ≤ 0.05) reduced lesion length and intensity of the pathogen action when recorded after 7 days under polyhouse conditions. An increased activity of phenylalanine ammonia lyase, polyphenol oxidase and superoxide dismutase (defence enzymes) was also recorded in chitosan-treated maize plants. Thus, chitosan showed promising management potential against banded leaf and sheath blight of maize caused by RSS under in vitro and in vivo trials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.

Similar content being viewed by others

REFERENCES

  1. Hochholdinger, F. and Tuberosa, R., Curr. Opin. Plant Biol., 2009, vol. 12, pp.172–177.

    Article  CAS  PubMed  Google Scholar 

  2. Devi, B. and Thakur, B.R., Proc. Nat. Acad. Sci., India Sect. B Biol. Sci., 2018, vol. 88, pp. 769–777.

    Article  Google Scholar 

  3. Singh, S.K., Patel, M.B., Thakker, B.N., Hooda, K.S. and Barad, A.K., Int. J. Curr. Microbiol. Appl. Sci., 2019. vol. 8, no. 7, pp. 2858–2866. https://doi.org/10.20546/ijcmas.2019.807.356

    Article  CAS  Google Scholar 

  4. Stresses of Maize in Tropics, Zaidi, P.H. and Singh, N.N., Eds., New Delhi: Directorate of Maize Research, 2005, pp. 159–171.

  5. Hooda, K.S., Khokhar, M.K., Parmar, H., Gogoi, R., Joshi, D., Sharma S.S., et al., Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci., 2017, vol. 87, pp. 1041–1052. https://doi.org/10.1007/s40011-015-0688-5

    Article  Google Scholar 

  6. Rabea, E.I., Badawy, M.E.T., Stevens, C.V., Smagghe, G., and Steurbaut, W., Biomacromolecules, 2003, vol. 4, pp. 1457–1465.

    Article  CAS  PubMed  Google Scholar 

  7. Rabea, E.I., Badawy, M.E.T., Steurbaut, W., and Stevens, C.V., Eur. Polym. J., 2009, vol. 45, pp. 237–245.

    Article  CAS  Google Scholar 

  8. El-Hadrami, A., Adam, L.R., El Hadrami, I., and Daayf, F., Mar. Drugs, 2010, vol. 8, pp. 968–987.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Reddy, M.B., Angers, P., Castaigne, F., and Arul, J., J. Am. Soc. Hortic. Sci., 2000, vol. 125, no. 6, pp. 742–747.

    Article  CAS  Google Scholar 

  10. Devlieghere, F., VermeuleA., and Debevere, J., Food Microbiol., 2004, vol. 21, no. 6, pp. 703–714.

    Article  CAS  Google Scholar 

  11. Onaran, A., Bayar, Y., Karakurt, T., Tokatl, K., Bayram, M., and Yanar, Y., J. Taibah Univ. Sci., 2021, vol. 15, no. 1, pp. 852–860. https://doi.org/10.1080/16583655.2021.2006434

    Article  Google Scholar 

  12. Mohammed, S.R., Zeitar, E.M., and Eskov, I.D., Open Agric. J., 2019, vol. 13, no. 1.

  13. Hoang, N.H., Le Thanh, T., Sangpueak, R., Treekoon, J., Saengchan, C., Thepbandit, W., et al., Polymers, 2022, vol. 14, p. 662. https://doi.org/10.3390/polym14040662

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Pandey, D.K., Tripathi, N.N., Tripathi, R.D., and Dixit, S.N., J. Plant Dis. Prot., 1982, vol. 89, pp. 344–349.

    Google Scholar 

  15. Liu, H., Tian, W., Li, B., Wu, G., Tao, Z., Wang, Y., et al., Biotechnol. Lett., 2012, vol. 34, no. 12, pp. 2291–2298.

    Article  CAS  PubMed  Google Scholar 

  16. Imoto, T. and Yagishita, K., Agric. Biol. Chem., 1971, vol. 35, no. 7, pp. 1154–1156.

    Article  CAS  Google Scholar 

  17. Koukol, J. and Conn, E.E., J. Biol. Chem., 1961, vol. 236, no. 10, pp. 2692–2698.

    Article  CAS  PubMed  Google Scholar 

  18. Dhindsa, R.H., Plumb-Dhindsa, R. and Thorpe, T.A., J. Exp. Bot., 1981, vol. 32, p. 93101.

    Google Scholar 

  19. Manjunatha, G., Niranjan-Raj, S., Prashanth, G.N., Deepak, S., Amruthesh K.N., and Shetty H.S., Pest. Manage. Sci., 2009, vol. 65, pp. 737–743.

    Article  CAS  Google Scholar 

  20. Manjunatha, G., Roopa, K.S., Prashanth, G.N., and Shetty, H.S., Pest Manage. Sci., 2008, vol. 64, pp. 1250–1257.

    Article  CAS  Google Scholar 

  21. Shih, P.Y., Liao, Y.T., Tseng, Y.K., Deng, F.S., and Lin, C.H., Front. Microbiol., 2019, vol. 10, p. 602.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Al-Hetar, M.Y., Zainal Abidin, M.A., Sariah, M., and Wong, M.Y., J. Appl. Polymer Sci., 2011, vol. 120, no. 4, pp. 2434–2439.

    Article  CAS  Google Scholar 

Download references

ACKNOWLEDGMENTS

The first author is grateful to ICAR-Indian Agricultural Research Institute, New Delhi for the research facilities provided for the research work.

Author information

Authors and Affiliations

  1. ICAR-National Research Centre for Integrated Pest Management, 110012, New Delhi, India

    R. Balodi, S. Singh & S. K. Singh

  2. ICAR-Indian Agricultural Research Institute, Pusa Campus, 110012, New Delhi, India

    R. Balodi, R. Gogoi & S. Bisht

  3. Rani Laxmi Bai Central Agricultural University, 284001, Jhansi, Uttar Pradesh, India

    S. Bisht

Authors
  1. R. Balodi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Gogoi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Bisht
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. K. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to R. Balodi or R. Gogoi.

Ethics declarations

The authors declare that they have no conflicts of interest.

This article does not contain any studies with animals or human participants performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Balodi, R., Gogoi, R., Bisht, S. et al. Antifungal Activity of Chitosan Against Rhizoctonia solani f.sp. sasakii. Appl Biochem Microbiol 59, 323–329 (2023). https://doi.org/10.1134/S000368382303002X

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S000368382303002X

Keywords:

Search

Navigation