Abstract
103 bacterial isolates obtained from 8 ethnomedicinal plants in Manipur, India were studied for antifungal and plant growth promoting (PGP) activities. Forty-six (46), out of 62 antifungal isolates, showed potent activities against R. solani. Since R. solani (RS), a sheath blight pathogen, threatens rice yields worldwide, the present study was aimed at discovering promising bioinoculants with anti-RS and PGP potential on rice. Twenty-nine (29) endophytic isolates exhibiting promising anti-RS and PGP activities were subjected to seed vigor assays on rice (var. Jatra) and 16 were found to enhance rice seedling vigour by 70% or more over the control. Four (4) strains, Streptomyces sp. (AcRz21), Alkalihalobacillus sp. (PtL11), Bacillus sp. (TgIb5), and Priestia sp. (TgIb12) with the highest vigor indices were studied for growth promotion of rice in field conditions under pathogen-challenged and pathogen-free conditions. These bioactive strains were able to significantly enhance root and shoot biomass and reduce lesion heights caused by R. solani.
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Acknowledgements
The authors gratefully acknowledge the financial support of the Department of Biotechnology (DBT), Government of India (GOI) under the Twinning Programme for the NE scheme (BT/PR25395/NER/95/1177/) and Advanced Level State Biotech Hub (AdL SBT Hub) Scheme (BT/04/NE/2009) that partly facilitated this work.
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Debananda S. Ningthoujam (DSN) conceived the idea of the project, guided the overall work, and edited the Ms. through several rounds of editing. Rakhi Khunjamayum conducted all the experiments, wrote the initial draft of the manuscript and prepared the tables and figures. K. Tamreihao helped in the design of the field experiments and in writing the initial manuscript. Roshan S. Asem and Y. Rojiv Singh assisted in the field experiments. Amanda Nongthombam, K. Mrinalini Devi, and Th. Pooja Devi helped in the PGP screening assays and field experiments. L. Jaya Devi also assisted in the field trials. Saikat Mukherjee helped in preparing the initial manuscript. DSN also took the main role in finalizing discussion of the results and refining & editing the Ms. to the final version.
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Khunjamayum, R., Tamreihao, K., Asem, R.S. et al. Fungal disease suppression and growth promotion potential of endophytic bacteria from ethnomedicinal plants. Arch Microbiol 204, 539 (2022). https://doi.org/10.1007/s00203-022-03136-w
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DOI: https://doi.org/10.1007/s00203-022-03136-w