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Influence of Mono- and Oligosaccharides on cbh1 Gene Transcription in the Filamentous Fungus Penicillium verruculosum

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Abstract

A method has been developed for the analysis of the induction of cbh1 gene transcription in the filamentous fungus Penicillium verruculosum after treatment with mono- and oligosaccharides. The method makes it possible to obtain mRNA, which encodes cellobiohydrolase 1 (CBH1), in an amount sufficient for RT-qPCR. Citrate, a compound that does not cause carbon catabolite repression, was used as a carbon source. It was shown for the first time that xylose, gentiobiose, and, mainly, cellobiose induce the expression of the cbh1 gene in P. verruculosum, while sophorose and a mixture of xylooligosaccharides with a degree of polymerization of 3–5 most likely serve as precursors of inducers.

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Funding

The work was supported by the Russian Foundation for Basic Research (project no.18-29-07070).

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Authors and Affiliations

  1. Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071, Moscow, Russia

    V. Yu. Kislitsin, A. M. Chulkin, I. N. Zorov, I. A. Shashkov, A. D. Satrutdinov, A. P. Sinitsyn & A. M. Rozhkova

  2. Chemistry Faculty, M.V. Lomonosov Moscow State University, 119991, Moscow, Russia

    I. N. Zorov & A. P. Sinitsyn

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  1. V. Yu. Kislitsin
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Correspondence to A. M. Rozhkova.

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Translated by I. Gordon

Abbreviations: CBH1—cellobiohydrolase 1; CF—culture fluid; COS—cellooligosaccharides; EDTA—ethylenediaminetetraacetate; HPLC—high-performance liquid chromatography; MCC—microcrystalline cellulose; MCC-AO—active orange-stained MCC; RT-qPCR—reverse transcription quantitative real time polymerase chain reaction; SDS—sodium dodecyl sulfate; XOS—xylooligosaccharide.

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Kislitsin, V.Y., Chulkin, A.M., Zorov, I.N. et al. Influence of Mono- and Oligosaccharides on cbh1 Gene Transcription in the Filamentous Fungus Penicillium verruculosum. Appl Biochem Microbiol 57, 925–932 (2021). https://doi.org/10.1134/S0003683821090040

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