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Development of an immunochromatographic test system for the detection of Helicobacter pylori antigens

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Abstract

A test system based on immunochromatography in the sandwich format and intended for express detection of Helicobacter pylori antigens has been developed. Contact of a sample with a test strip coated with immunochemical reagents triggers the movement of the liquid along the membrane components of the test strip, immunochemical interactions, and the formation of detection zones stained by gold nanoparticles. The concentration and kinetic dependences of the immunochemical interactions have been characterized. The reagent and membrane composition of the test system has been selected to provide a minimal detection limit. The detection of H. pylori cell wall antigens at concentrations as low as 0.3 μg/mL in aqueous solution and a suspension of a clinical sample of feces has been demonstrated; the assay duration was 10 min. Staining enhancement by the addition of silver salts allowed for a further reduction of the detection limit to 0.03 μg/mL. The developed test system can be used for field diagnostics.

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Authors and Affiliations

  1. Bach Institute of Biochemistry of the Russian Academy of Sciences, Moscow, 119071, Russia

    N. A. Byzova, A. V. Zherdev, E. G. Sadykhov & B. B. Dzantiev

  2. All-Russia Research Center of Molecular Diagnostics and Therapy, Moscow, 117638, Russia

    P. G. Sveshnikov

Authors
  1. N. A. Byzova
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  2. A. V. Zherdev
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  3. P. G. Sveshnikov
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  4. E. G. Sadykhov
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  5. B. B. Dzantiev
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Corresponding authors

Correspondence to N. A. Byzova or P. G. Sveshnikov.

Additional information

Original Russian Text © N.A. Byzova, A.V. Zherdev, P.G. Sveshnikov, E.G. Sadykhov, B.B. Dzantiev, 2015, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2015, Vol. 51, No. 5, pp. 520–530.

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Byzova, N.A., Zherdev, A.V., Sveshnikov, P.G. et al. Development of an immunochromatographic test system for the detection of Helicobacter pylori antigens. Appl Biochem Microbiol 51, 608–617 (2015). https://doi.org/10.1134/S000368381505004X

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  • DOI: https://doi.org/10.1134/S000368381505004X

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