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Novel immobilized biocatalyst for microbiological synthesis of pharmaceutical steroids

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Abstract

The steroid-transforming activity of free and immobilized cells of Pimelobacter simplex VKPM Ac-1632 entrapped in an operationally stable macroporous poly(vinyl alcohol) cryogel was studied. It was shown that the macroporous matrix of the carrier did not create any diffusional limitations for steroid access to the cells or the removal of the transformation product from them. The optimal conditions for the hydrocortisone 1,2-dehydrogenation into prednisolone by free and immobilized cells were elucidated. The immobilized biocatalyst was obtained in a granulated form and used in 32 successive cycles of steroid transformation. The average cycle duration was 45 min, and the prednisolone yield of during the first 20 cycles was 98%. It was established that the immobilized cells of the actinobacteria P. simplex retained high steroid-transforming activity over all of the transformation cycles. The physicochemical and diffusion characteristics of the polyvinyl alcohol gels and its granules were determined, and their high stability during repeated cycles of steroid transformation was shown. The results indicated that P. simplex immobilized cells represent an effective catalyst suitable for multiple use. Biomass consumption decreased upon its use, and product isolation, as well as culture storage, was much easier.

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Authors and Affiliations

  1. Bioengineering Center, Russian Academy of Sciences, Moscow, 117312, Russia

    V. A. Andryushina, N. V. Karpova, A. V. Druzhinina & T. S. Stytsenko

  2. Nesmeyanov Institute of Organoelemental Compounds, Russian Academy of Sciences, Moscow, 119991, Russia

    E. A. Podorozhko, A. N. Ryabev & V. I. Lozinsky

Authors
  1. V. A. Andryushina
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  2. N. V. Karpova
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  3. A. V. Druzhinina
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  4. T. S. Stytsenko
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  5. E. A. Podorozhko
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  6. A. N. Ryabev
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  7. V. I. Lozinsky
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Corresponding author

Correspondence to V. A. Andryushina.

Additional information

Original Russian Text © V.A. Andryushina, N.V. Karpova, A.V. Druzhinina, T.S. Stytsenko, E.A. Podorozhko, A.N. Ryabev, V.I. Lozinsky, 2015, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2015, Vol. 51, No. 5, pp. 472–481.

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Andryushina, V.A., Karpova, N.V., Druzhinina, A.V. et al. Novel immobilized biocatalyst for microbiological synthesis of pharmaceutical steroids. Appl Biochem Microbiol 51, 530–538 (2015). https://doi.org/10.1134/S0003683815050026

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  • DOI: https://doi.org/10.1134/S0003683815050026

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