Abstract
Obligate root holoparasite Phelipanche aegyptiaca is an agricultural pest, which infests its hosts and feeds on the sap, subsequently damaging crop yield and quality. Its notoriously viable seed bank may serve as an ideal pest control target. The phytohormone abscisic acid (ABA) was shown to regulate P. aegyptiaca seed dormancy following strigolactones germination stimulus. Transcription analysis of signaling components revealed five ABA receptors and two co-receptors (PP2C). Transcription of lower ABA-affinity subfamily III receptors was absent in all tested stages of P. aegyptiaca development and parasitism stages. P. aegyptiaca ABA receptors interacted with the PP2Cs, and inhibited their activity in an ABA-dependent manner. Moreover, sequence analysis revealed multiple alleles in two P. aegyptiaca ABA receptors, with many non-synonymous mutations. Functional analysis of selected receptor alleles identified a variant with substantially decreased inhibitory effect of PP2Cs activity in-vitro. These results provide evidence that P. aegyptiaca is capable of biochemically perceiving ABA. In light of the possible involvement of ABA in parasitic activities, the discovery of active ABA receptors and PP2Cs could provide a new biochemical target for the agricultural management of P. aegyptiaca. Furthermore, the potential genetic loss of subfamily III receptors in this species, could position P. aegyptiaca as a valuable model in the ABA perception research field.
Similar content being viewed by others
Introduction
The obligate root holoparasite weed Phelipanche aegyptiaca (Egyptian broomrape), is a species from the Orobanchaceae family, which includes some of the most agriculturally damaging weeds1,2,3. P. aegyptiaca is harmful, especially owing to its ability to parasitize a large variety of crop families, including Solanaceae, Brassicaceae, Cucurbitaceae, Cruciferae, Apiaceae, Fabaceae and Asteraceae4. As an obligate holoparasite lacking chlorophyll and effective roots, P. aegyptiaca depends entirely on its host for nutrients and water5. Germination of P. aegyptiaca and other obligate Orobanchaceae parasites requires strigolactones, stimuli derived from the host plant6. Exposure of conditioned P. aegyptiaca and P. ramose seeds to a synthetic germination stimulant similar in structure to strigolactones, was rapidly followed by a considerable elevation in CYP707A1 transcript levels and reduction of seed abscisic acid (ABA) content7,8,9. Cytochrome P450 CYP707A encodes ABA 8′-hydroxylase, a key enzyme in ABA catabolism, and plays a role in relieving seed dormancy10. Thus, an antagonistic relationship between strigolactones and the germination inhibitor ABA stand at the basis of germination regulation in parasitic Orobanchaceae species.
ABA elicits its effect by binding pyrabactin resistance1/PYR1-like/regulatory component of ABA receptor (PYR/PYL/RCAR) ABA receptors in a large and conserved hydrophobic pocket, which changes the conformation of two highly conserved loops located in the outer periphery of the pocket11,12,13. Both loops, the “gate” and the “latch”, move towards the ligand and in doing so “cover” the pocket cavity13. This structural shift enables the occupation of the catalytic core of protein phosphatases type 2CA (PP2CA), the ABA co-receptors, in a manner which blocks its activity13. Arabidopsis thaliana PP2CA ABI1 and ABI2 were the first confirmed negative regulators of ABA signaling14, where the ABA-mediated interaction between PYR/PYLs and ABI1/ABI2 was shown to inhibit the phosphatase activity in-vitro and to antagonize their action in-planta11,12.
Many of the ABA receptor functions can be attributed to their sensitivity to ABA and affinity to PP2C, i.e., the concentration of ABA which elicits a receptor-PP2C interaction. Dimeric ABA receptors have been shown to require higher concentrations of ABA to elicit the same activity as monomeric receptors15. In Arabidopsis, deficiency in three dimeric receptors was associated with measurable ABA insensitivity and a reduced inhibitory effect of ABA on seed germination12.
Autotroph plants utilize ABA as an inductive signal in a wide range of responses and physiological functions vital to their survival and reproduction. Few of these functions, e.g., stress-related responses in the roots and leaves, have not been identified in obligate holoparasitic plants, such as P. aegyptiaca that rely completely on their host for continuous supply of water and nutrients. The reduction in ABA-related functions in holoparasitic plants might correspond with some degree of degeneration in the ABA signal transduction pathway. This hypothesis is strengthened by the recent identification and classification of ABA receptors and co-receptors in the hemiparasitic Orobanchaceae species Striga hermonthica, which transcribes four ABA co-receptors, including one which is mutated in such way that it effectively blocks ABA signaling9.
In this study, we explored ABA perception in P. aegyptiaca and provide early insights into genetic variance and its functionality in a wild species. Alongside the potential evolutionary implications of such discoveries, the insights may also illuminate new approaches for agrotechnical control of this pest.
Results
P. aegyptiaca transcribes core ABA signaling components
The basis of the biochemical response to ABA is facilitated by an interaction between ABA and its receptor, followed by the ABA-receptor inhibitory effect on a co-receptor (PP2CA). Identification of these components in P. aegyptiaca was based on sequence homology with Arabidopsis ABA receptor PYR1 and ABA co-receptor ABI1. In the absence of a publically available sequenced genome, the Parasitic Plant Genome Project (PPGP) EST database is currently the most extensive source of information about P. aegyptiaca genetics. The database contains cDNA sequences obtained from P. aegyptiaca and two other parasitic plant species at specific developmental stages and from different tissues16.
In-silico analysis of P. aegyptiaca transcript data revealed five putative ABA receptors (PaPYL4-8) and five putative ABA PP2C co-receptors (Figs 1 and S1). Regions predicted to be key to receptor functionality13,17 in the amino acid sequences of the putative ABA receptors were found to be highly similar to those of the Arabidopsis ABA receptors (Fig. 1). One exception was PaPYL5, which varied from both Arabidopsis and the rest of the P. aegyptiaca receptors in a highly conserved region, which includes the “latch” loop (PYR1 H115, R116 and L117) (Fig. 1). The latch is one of two surface loops that bind ABA and co-receptors. A change in this region may therefore affect ABA receptor function.
The individual transcription pattern of the putative ABA receptors and co-receptors was analyzed using the publically available PPGP transcriptome data, which are categorized by developmental stage and tissue type (summarized on Fig. 2). Results showed that at least two ABA receptors are transcribed at any given stage of P. aegyptiaca life cycle, which can be an indication of active ABA perception. PaPYL6 and the ABI-like 2 co-receptor (PaABIL2) were only transcribed during seed germination and early established parasite stage. PaABIL2 was also transcribed during “post-emergence from soil” stage.
None of the transcribed P. aegyptiaca ABA receptors classify as a subfamily III ABA receptor
In-silico phylogenetic analysis clustered PaPYL4-6 with subfamily II of A. thaliana ABA receptors, and PaPYL7 and 8 with subfamily I (Fig. 3). None of the putative P. aegyptiaca ABA receptors clustered with subfamily III, an unusual finding as compared to ABA receptor expression analyses in other higher plants9,18,19,20,21,22,23. Thus we decided to number the receptors in accordance to Arabidopsis subfamily clustering. Subfamily II receptors were named PaPYL4, 5 and 6 and Subfamily I receptors were named PaPYL7 and PaPYL8.
A functional analysis was then performed to confirm the computational phylogenetic classification of putative P. aegyptiaca ABA receptors into subfamilies I and II. To this end, the five receptors were cloned from plant samples collected in Israel. The cloned receptors were highly similar to the PPGP database sequences, with the exception of PaPYL5. The latch loop of this variant, unlike its PPGP counterpart, was found to be conserved as compared to other functional receptors. This version was named PaPYL5 JV after the source of the sample - Jezreel Valley.
The interactions between ABA receptors and A. thaliana ABA co-receptors ABI1 and its mutant ABI1G180D (encoded by abi1-1) in a yeast two-hybrid assay, can be used as an indication of a subfamily affiliation22. PaPYL4 and PaPYL5 interacted with ABI1 in an ABA-independent manner, while the interaction with ABI1G180D was ABA-dependent (Fig. 4), which coincided with the characteristics of the A. thaliana ABA receptor subfamily II. PaPYL6-8 interacted with both ABI1 and ABI1G180D in an ABA-independent manner, in accordance with the characteristics of the A. thaliana ABA receptor subfamily I.
In order to determine whether the absence of subfamily III transcription is a common feature of parasitic plants, sequences encoding putative ABA receptors of the following species were analyzed in-silico: obligate root hemiparasite Striga hermonthica9, facultative root hemiparasite Triphysaria versicolor (Orobanchaceae, EST libraries available in the PPGP website) and obligate stem holoparasites Cuscuta pentagona and Cuscuta suaveolens24 (EST libraries available in the GenBank TSA database). In all tested species, unlike in P. aegyptiaca, at least one putative ABA receptor clustered with the subfamily III ABA receptor family (Fig. 3).
P. aegyptiaca ABA receptors interact with P. aegyptiaca ABA co-receptors and inhibit their activity in an ABA-dependent manner
Of the five putative ABA co-receptors identified in the in-silico analysis of the P. aegyptiaca transcriptome, only two were shown to interact with P. aegyptiaca ABA receptors in the yeast two-hybrid assay (Fig. 5). Furthermore, only these two co-receptors interacted with Arabidopsis SnRK (Fig. S1). The interaction between P. aegyptiaca ABI like 1 (PaABIL1) and PaPYL4-8 was ABA-independent. PaABIL2 only interacted with PaPYL6. The other three putative P. aegyptiaca clade A subfamily of type II C protein phosphatases like 1–3 (PaPP2CAL1-3) ABA co-receptors did not interact with any of the receptors. A receptor-mediated phosphatase activity assay performed to further investigate the interaction between recombinant PaABIL1 and PaPYL4-8 showed that PaPYL4, PaPYL5, PaPYL7 and PaPYL8 inhibited the de-phosphorylation activity of PaABIL1 in an ABA dose-dependent manner (Fig. 5).
Allelic variations in PaPYL4 affect its interaction with co-receptors
As part of the characterization of P. aegyptiaca ABA receptors, multiple genes encoding PaPYL4-5 originating from the Jezreel Valley (32° 35′ 47″N, 35°14′31″E region) population, were cloned and sequenced. In-silico analysis revealed that PaPYL4 and PaPYL5 presented both synonymous and non-synonymous mutations. Amongst PaPYL4 clones, 35 different alleles were discovered, 6 of which had nucleotide insertions or deletions resulting in a frame-shift. PaPYL5 clones included 12 different alleles, one with a frame-shift and three with nonsense mutations. Assessment of the interaction between the 11 PaPYL4 alleles with complete open reading frames and ABA co-receptors (PaABIL1, PaABIL2, HAB1, ABI1, ABI1G180D, ABI2 and ABI2G168D) in a yeast two-hybrid assay (Fig. S2), showed that the allelic variation did not affect this interaction, regardless of ABA concentration. However, variant PaPYL4.2 showed a substantially decreased interaction with all the ABA co-receptors, manifested by higher ABA concentration requirements, and failure to interact with the mutated co-receptors at any tested concentration of ABA. In comparison to the normally interacting receptor, PaPYL4.1, PaPYL4.2 displayed no in-vitro PP2C inhibition activity, even in the presence of 5 µM of ABA (Fig. S3).
Discussion
This work presented evidence of the capacity of an obligate holoparasitic plant, Phelipanche aegyptiaca, to biochemically perceive ABA signaling, by the apex components of the ABA signal transduction pathway. Through characterization of the plant’s ABA receptors and co-receptors and comparison with homologous autotrophic angiosperm genes, we propose a possible deterioration of the P. aegyptiaca ABA perception mechanism. This might be the result of the evolutionary transition of this species from self-dependence to parasitism, in which loss of redundancies in once critical traits can occur without decreasing fitness.
The first and most prominent element hinting to a reduction in P. aegyptiaca ABA perception, was the absence of subfamily III ABA receptor transcription. This was unique as compared to other species of higher plants, which consistently expressed receptors of all three ABA receptor subfamilies9,18,19,20,21,22,23. Arabidopsis ABA receptor subfamily III is comprised of dimeric receptors15,25, which, recent data suggest, are main mediators of the downstream transcription effect of ABA26. Activation of dimeric receptors requires higher levels of ABA in comparison to monomeric receptors, suggestive of an advanced, modular response mechanism. Evidence of subfamily III receptor transcription in Orobanchaceae hemiparasitic species and in other holoparasitic plants, suggests that the absence of transcription might be limited to Orobanche species, or perhaps only to P. aegyptiaca. This possibility could be explored pending release of the sequenced genomes of P. aegyptiaca and other Orobanche species, which will allow us to unequivocally determine whether subfamily III genes are present, lost or merely not transcribed. Loss of the gene expression would coincide with previous evidence of key autotrophic genes which are also not transcriptionally active in the Orobanchaceae family27,28. Secondly, nearly a quarter of the discovered PaPYL4 alleles are likely to encode incomplete proteins caused by small insertions or deletions. Amongst the twelve different alleles with a full coding sequence and evaluated for interaction with co-receptors in the presence of a range of ABA concentrations, only PaPYL4 exhibited reduced affinity to ABA co-receptors. The presence of numerous inactive alleles, together with the high proportion of alleles which most probably encode non-functioning proteins, is a strong indication of a relaxed selection of PaPYL4. As with PaPYL4, the PaPYL5 coding sequence obtained from the PPGP database, also seemed to be the product of low selective pressure, which enabled vast mutation of a highly conserved region, including the “latch”, one of two surface loops that bind ABA and co-receptors.
Nonetheless, ABA clearly plays a major regulatory role in P. aegyptiaca seed dormancy, and likely in other parts of the life cycle, as could be deduced from ABA receptor and co-receptor transcription throughout multiple developmental stages. This, together with transcription of ABA biosynthesis components, signifies the prominence of ABA even in an obligate holoparasitic plant. However, many aspects of the role of ABA in P. aegyptiaca are yet to be understood, especially in the parasitism dynamics with the host plant. The new information gained here could provide a basis for further exploration of ABA involvement in parasitism mechanisms in plants, in general, and of Orobanche physiology, in particular. Moreover, structural data of functional P. aegyptiaca ABA receptors might serve as a scaffold to engineer selective agonists that differentially affect P. aegyptiaca without harming the host. As ABA inhibits germination and growth, such agonists can provide a new strategy for pest management.
Methods
Identification of putative ABA receptor and ABA co-receptor sequences
The PYR1 and ABI1 amino acid sequences were obtained from The Arabidopsis Information Resource (Loci AT4G17870.1 and AT4G26080 respectively). These sequences were used to query (TBLASTN) the Parasitic Plant Genome Project database16 for homologous nucleic acid sequences in P. aegyptiaca, T. versicolor and S. hermonthica, and the GenBank TSA database for C. pentagona and C. suaveolens sequences. Expressed sequence tag (EST) sequences overcoming the e-value 1.0−10 threshold, were imported to Geneious® 7.1.9 (https://www.geneious.com) and were assembled using the De Novo Assemble tool (default settings). Open reading frames in the assembled sequences were predicted, translated to amino acids (Standard Code/transl_table 1) and aligned (pairwise MUSCLE alignment, default settings) to PYR1 or ABI1 using Geneious® 7.1.9. Since, in some cases, the genetic material was extracted from tissue connected to the host plant, a basic local alignment search (Standard Nucleotide BLAST) of the PYR1 and ABI1 homologous sequences was conducted using the NCBI database. Cases with high identity with the host species were excluded. In order to identify the tissues and developmental stages in which any ABA receptor or co-receptor were likely to be transcribed, the newly identified sequences were used to query (BLASTN) the PPGP database for homologous nucleic acid sequences in P. aegyptiaca. In some cases, highly similar sequences were identified via the database nucleotide sequence pairwise alignments, yet some variation (no greater than 5% of the entire sequence) was present. We attributed this to the large allelic variation we observed in our own in-vitro experiments, and decided to include these less than perfect matches in the transcription pattern in the presented results.
Phylogenetic analysis
Phylogenetic trees were inferred using the MEGA6.06 software29, based on the MUSCLE method30 using the UPGMB clustering method. The START domain of AtPYR1 (38–172) was used as a reference for identification of the START domain of other proteins. The evolutionary history was inferred using the Neighbor-Joining method31. Percentage of 1000 replicate trees in which the sequences clustered together in the bootstrap test, is shown next to the branches32.
Sources of P. aegyptiaca RNA and tissue samples
For the initial cloning, P. aegyptiaca tissues (tubercles, young shoots and flowers) were harvested from plants originating from seeds collected in Ramat-David (Israel) in July 2014. The plants were grown with and parasitized onto tomato cultivar Solanum Lycopersicum MP-1 sp. Total RNA was extracted from tubercles, young shoots and flowers using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, catalog number STRN50), according to the manufacturer’s instructions. The RNA samples, frozen (−20 °C) tissue and source seeds were kindly provided by Dr. Radi Aly of the Newe Ya’ar Research Center (ARO). cDNA was synthesized from the RNA samples using the SuperScript® III First-Strand Synthesis System for RT-PCR (Invitrogen, catalog number 18080051), according to the manufacturer’s instructions.
Growth conditions of P. aegyptiaca
P. aegyptiaca seeds were mixed in soil (8 g seeds per 1 L soil) and transferred to 4 L pots, into which two-week-old tomato cultivar Solanum Lycopersicum M82 sp were planted. The inoculated plants were grown under greenhouse conditions (natural day length, 25 °C/20 °C day/night temperature). The first P. aegyptiaca flowers broke soil during the third month of the growing period. Tissue samples of the flowers and the stems were collected during the following month, and stored at −80 °C.
DNA extraction and amplification
P. aegyptiaca tissue samples were ground to powder using a TissueLyser II (QIAGEN). Samples were mixed with 600 µl DNA extraction buffer and incubated at 65 °C, for 30 min. Chloroform (600 µl) was then added to the samples, which were then centrifuged at 20,000 RCF, for 2 min. The upper phase was isolated and mixed with 600 µl chloroform and centrifuged at 20,000 RCF, for 2 min. The upper phase was isolated again, mixed with isopropanol at a 2:3 ratio, and stored for a least 30 min, at −20 °C. The samples were then centrifuged at 20,000 RCF, for 30 min. The supernatant was discarded and the pellet was washed (not resuspended) with 600 µl cold (−20 °C) 70% ethanol. The samples were then centrifuged at 20,000 RCF, for 5 min, and the supernatant was discarded. In order to remove residual ethanol, the samples were incubated at 60 °C, until the pellet fully dried. The pellet was then resuspended in water.
Selected genes were amplified using Phusion® High-Fidelity DNA Polymerase (New England BioLabs, catalog number M0530L), according to the manufacturer’s instructions. All gene primers were designed using Primer3 version 2.3.4, via Geneious® 7.1.9, and are listed in Table S1.
Yeast-based receptor activation assays
The coding sequences of PaPYL4-8 were fused to the GAL4 DNA-binding domain (GBD) coding sequence, by Gibson assembly (New England BioLabs protocol E5510), in a pBD-GAL4 CAM vector (Clontech), restricted by SalI and EcoRI. The coding sequences of PaABIL1-2, PaPP2CAL1-3, ABI1 – AT4G26080, ABI1G180D (abi1-1), ABI2 – AT5G57050, ABI2G168D (abi2-1) and HAB1 – AT1G72770 were fused to the GAL4-activating domain (GAD) coding sequence by ligation with pACT2 (Clontech), restricted by MfeI and XmaI. The assembled and ligated vectors were cloned and propagated in Escherichia coli (DH5α) and then transferred to Saccharomyces cerevisiae strain Y190. Transformed yeast were selected for vector presence on synthetic defined medium (SD) lacking leucine and tryptophan. Interaction between PaPYL4-8 and PP2Cs was detected by inculcating individual clones onto plates supplemented with 0.1–10 µM ABA (Biosynth, Switserland) or 0.1% DMSO as solvent control (mock) (48 h, at 30 °C) and then monitoring β-galactosidase reporter gene expression levels through X-Gal staining, as described by Park et al. (2009). The assay, for all clones was repeated at least three times.
In-vitro receptor activation assay/phosphatase inhibition assay
Protein expression
The PaPYL4.1, PaPYL4.2 and PaPYL5 coding sequences were cloned into the pSUMO vector. The PaPYL7, 8 and PaABIL1 coding sequences were Gibson-assembled into a modified pGEX-4T-1 vector (Δ863–893). The constructs were transformed into E. coli BL21 (DE3) pLysS bacteria by heat-shock. Protein purification was performed with the methods described in Pri-Tal et al.22.
Phosphatase inhibition assay
The inhibitory effect of PaPYL4.1, 4.2, 7 and PaPYL8 on PaABIL1 was measured by reduction in ability of the phosphatase to catalyze the hydrolysis of p-nitrophenyl phosphate (pNPP). The hydrolysis product, p-nitrophenol, is chromogenic and can be detected via spectrophotometry (wavelength of maximum absorbance is 405 nm). Each 100 µl reaction contained 120 nM GST-PaABIL1 with 360 nM 6XHIS-SUMO-PaPYL4.1 or 6XHIS-SUMO-PaPYL5 or 200 nM GST-PaABIL1 with 600 nM GST-PaPYL7 or GST-PaPYL8, in 33 mM Tris·acetate, pH 7.9, 66 mM potassium acetate, 0.1% (w/v) BSA, 10 mM MnCl2, 0.1% (v/v) 2-mercaptoethanol and 50 mM pNPP. Each reaction was supplemented with ABA, dissolved in DMSO (0.1, 0.5, 5 µM), or with 1% (v/v) DMSO only (mock). One of the reactions did not contain any receptor, in order to measure the basal activity of the phosphatase. The hydrolysis reaction was measured every 60 sec, for 20 min, for 6XHIS-SUMO-PaPYL4.1 and 6XHIS-SUMO-PaPYL5, and every 30 sec, for 30 min, for GST-PaPYL7 and GST-PaPYL8. Phosphatase activity was calculated by averaging two technical repetitions. Phosphatase activity levels are presented as a percentage of the phosphatase activity in the presence of a receptor without ABA (mock). This assay was reproduced with two independent protein preparations.
References
Bernhard, R. H., Jensen, J. E. & Andreasen, C. Prediction of yield loss caused by Orobanche spp. in carrot and pea crops based on the soil seedbank. Weed Res. 38, 191–197 (1998).
Parker, C. In Parasitic Orobanchaceae: parasitic mechanisms and control strategies (eds Joel, D. M., Gressel, J. & Musselman, L. J.) 313–345, https://doi.org/10.1007/978-3-642-38146-1 (Springer Heidelberg New York Dordrecht London, 2013).
Eizenberg, H. & Goldwasser, Y. Control of Egyptian Broomrape in Processing Tomato: A Summary of 20 Years of Research and Successful Implementation. Plant Dis. 102, 1477–1488 (2018).
Parker, C. Observations on the current status of orobanche and striga problems worldwide. Pest Manag. Sci. 65, 453–459 (2009).
Scholes, J. D., Quick, W. P. & Sciences, P. Interactions between the parasitic angiosperm Orobanche aegyptiaca and its tomato host: growth and biomass allocation. New Phytol. 133, 637–642 (1996).
Xie, X., Yoneyama, K. & Yoneyama, K. The Strigolactone Story Xiaonan. Annu. Rev. Phytopathol. 48, 93–117 (2010).
Yao, Z. et al. Global transcriptomic analysis reveals the mechanism of Phelipanche aegyptiaca seed germination. Int. J. Mol. Sci. 17 (2016).
Lechat, M.-M. et al. PrCYP707A1, an ABA catabolic gene, is a key component of Phelipanche ramosa seed germination in response to the strigolactone analogue GR24. J. Exp. Bot. 63, 695–709 (2012).
Fujioka, H. et al. Aberrant protein phosphatase 2C leads to abscisic acid insensitivity and high transpiration in parasitic Striga. Nat. Plants 5, 258–262 (2019).
Kushiro, T. et al. The Arabidopsis cytochrome P450 CYP707A encodes ABA 8′-hydroxylases: key enzymes in ABA catabolism. EMBO J. 23, 1647–56 (2004).
Ma, Y. et al. Regulators of PP2C Phosphatase Activity Function as Abscisic Acid Sensors. Science 324, 1064–1069 (2009).
Park, S.-Y. et al. Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins. Science 324, 1068–1071 (2009).
Melcher, K. et al. A gate–latch–lock mechanism for hormone signalling by abscisic acid receptors. Nature 462, 602–608 (2009).
Gosti, F. et al. ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 11, 1897–1910 (1999).
Dupeux, F. et al. A thermodynamic switch modulates abscisic acid receptor sensitivity. EMBO J. 30, 4171–4184 (2011).
Westwood, J. H. et al. The Parasitic Plant Genome Project: New Tools for Understanding the Biology of Orobanche and Striga. Weed Sci. 60, 295–306 (2012).
Dorosh, L., Kharenko, O. A., Rajagopalan, N., Loewen, M. C. & Stepanova, M. Molecular Mechanisms in the Activation of Abscisic Acid Receptor PYR1. PLoS Comput. Biol. 9, 1–17 (2013).
Sun, L. et al. Transcriptional regulation of SlPYL, SlPP2C, and SlSnRK2 gene families encoding ABA signal core components during tomato fruit development and drought stress. J. Exp. Bot. 62, 5659–5669 (2011).
Boneh, U., Biton, I., Zheng, C., Schwartz, A. & Ben-Ari, G. Characterization of potential ABA receptors in Vitis vinifera. Plant Cell Rep. 31, 311–321 (2012).
Kim, H. et al. A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a positive regulator of the ABA signal transduction pathway in seed germination and early seedling growth. J. Exp. Bot. 63, 1013–1024 (2012).
Fan, W. et al. Contrasting transcriptional responses of PYR1/PYL/RCAR ABA receptors to ABA or dehydration stress between maize seedling leaves and roots. BMC Plant Biol. 16, 1–14 (2016).
Pri-Tal, O., Shaar-Moshe, L., Wiseglass, G., Peleg, Z. & Mosquna, A. Non-redundant functions of the dimeric ABA receptor BdPYL1 in the grass Brachypodium. Plant J. 92, 774–786 (2017).
Cutler, S. R. et al. Tuning water-use efficiency and drought tolerance in wheat using abscisic acid receptors. Nat. Plants 5, 153–159 (2019).
Jiang, L. et al. Profiling mRNAs of two cuscuta species reveals possible candidate transcripts shared by parasitic plants. PLoS One 8 (2013).
Hao, Q. et al. The Molecular Basis of ABA-Independent Inhibition of PP2Cs by a Subclass of PYL Proteins. Mol. Cell 42, 662–672 (2011).
Vaidya, A. S. et al. A rationally designed agonist defines subfamily IIIA ABA receptors as critical targets for manipulating transpiration. ACS Chem. Biol. 12, 2842–2848 (2017).
Wolfe, A. D. & Claude, W. The Effect of Relaxed Functional Constraints on the Photosynthetic Gene rbcL in Photosynthetic and Nonphotosynthetic Parasitic Plants. 1243–1258 (1998).
Wicke, S. et al. Mechanisms of Functional and Physical Genome Reduction in Photosynthetic and Nonphotosynthetic Parasitic Plants of the Broomrape Family. Plant Cell 25, 3711–3725 (2013).
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725–2729 (2013).
Edgar, R. C. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792–1797 (2004).
Saitou, N. & Nei, M. The neighbor-joining method: a new method for reconstructing phylogenetic. Mol. Biol. Evol. 4, 406–425 (1987).
Felsenstein, J. Confidence Limits on Phylogenies: An Approach Using the Bootstrap. Evolution (N. Y). 39, 783–791 (1985).
Kai, W.-B. et al. Interactions of ABA signaling core components (SlPYLs, SlPP2Cs, and SlSnRK2s) in tomato (Solanum lycopersicon). J. Plant Physiol. 205, 67–74 (2016).
Acknowledgements
This work was supported by the ISF Israel Science Foundation (1069/14) and BARD (IS-4919-16 R).
Author information
Authors and Affiliations
Contributions
G.W. identified the receptors and conducted the phylogenetic, yeast-based receptor activation assays and the phosphatase inhibition assay. O.P.-T. conducted the yeast-based co-receptor and SnRK interaction assays and repeated several of the phosphatase inhibition assays. A.M. designed and supervised experiments. The manuscript was drafted by A.M. and G.W., and includes contributions from all co-authors. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Competing Interests
The authors declare no competing interests.
Additional information
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Wiseglass, G., Pri-Tal, O. & Mosquna, A. ABA signaling components in Phelipanche aegyptiaca. Sci Rep 9, 6476 (2019). https://doi.org/10.1038/s41598-019-42976-3
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-019-42976-3
- Springer Nature Limited