Abstract
Bacterial vaginosis (BV) is a gynecologic disorder characterized by a shift in cervicovaginal microbiota from Lactobacillus spp. dominance to a polymicrobial biofilm composed of diverse anaerobes. We utilized a well-characterized human three-dimensional cervical epithelial cell model in conjunction with untargeted metabolomics and immunoproteomics analyses to determine the immunometabolic contribution of three members of the Veillonellaceae family: Veillonella atypica, Veillonella montpellierensis and Megasphaera micronuciformis at this site. We found that Veillonella spp. infections induced significant elevation of polyamines. M. micronuciformis infections significantly increased soluble inflammatory mediators, induced moderate levels of cell cytotoxicity, and accumulation of cell membrane lipids relative to Veillonella spp. Notably, both V. atypica and V. montpellierensis infections resulted in consumption of lactate, a key metabolite linked to gynecologic and reproductive health. Collectively our approach and data provide unique insights into the specific contributions of Veillonellaceae members to the pathogenesis of BV and women’s health.
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Introduction
Human mucosae are colonized by diverse and dynamic bacterial communities that impact health and homeostasis or contribute to disease states, depending on the compositional nature of the communities1. In the cervicovaginal microenvironment, Lactobacillus dominance is associated with optimal reproductive health2. Lactic acid, a by-product of Lactobacillus fermentation, is an important metabolite in maintaining host defense and protecting the cervicovaginal mucosal epithelium from infection by sexually transmitted pathogens and opportunistic commensal bacteria3. Overgrowth of diverse anaerobic bacteria and depletion of Lactobacillus spp. is characteristic of bacterial vaginosis (BV) in the cervicovaginal microenvironment4,5. Our group and others have developed a hypothetical model suggesting that early colonizers, such as Gardnerella vaginalis and Prevotella bivia, establish biofilm scaffolds over the cervicovaginal epithelium and exhibit low pro-inflammatory properties4,6. This model proposes that as the biofilm matures, Lactobacillus spp. become less abundant and secondary colonizers associate with the biofilm, which results in inflammation4. As Lactobacillus spp. are depleted, BV-associated bacteria produce polyamines that result in increased pH4.
BV is a common and highly recurrent cervicovaginal disorder7. Additionally, BV has been associated with adverse obstetric and gynecologic outcomes, such as preterm birth, increased acquisition of sexually transmitted infections, and possibly gynecologic cancer8,9,10,11,12,13. It is therefore imperative to better understand the pathophysiological mechanisms of BV. Key bacteria that have been the focus of BV studies thus far include G. vaginalis, P. bivia, Sneathia amnii and Atopobium vaginae, although more studies are required in order to completely elucidate contributions of the cervicovaginal microbiome to women’s health10,14,15. However, other vaginal commensal bacteria and BV-associated bacteria have not been studied in detail and their roles in health or BV remain obscure. For example, members of the Veillonellaceae family, such as Veillonella atypica, Veillonella montpellierensis and Megasphaera micronuciformis, are understudied bacteria that have been isolated from the female reproductive tract16,17.
V. atypica is commonly isolated from the oral cavity and has been studied extensively at this anatomical site18,19,20,21,22. In the oral microbiome, V. atypica is considered a ‘bridging species’ that enables the colonization of middle and late colonizers to the oral plaque biofilm, with the aid of initial colonizing species, therefore V. atypica promotes oral biofilm development23,24,25. In addition, V. montpellierensis, another Veillonella species, has been isolated from patients suffering from endocarditis26 and acute otitis media infections27. Similar to V. atypica, M. micronuciformis has also been isolated from the oral and gut microbiomes and is implicated in gastric cancer and response to chemotherapy28,29,30.
M. micronuciformis and other Megasphaera spp. are typically isolated in relatively high frequency from patients with BV, along with Atopobium, Leptotrichia/Sneathia, and Eggerthella-like species31. In contrast, Veillonella spp. are isolated from women with and without BV and have been associated with elevated cervicovaginal pH31,32,33. Veillonella spp. are commonly isolated with Lactobacillus spp., G. vaginalis, and Peptostreptococcus spp31,32. Overall, Veillonellaceae family members have been isolated from women with BV16,17,32,34,35, but their contributions to BV pathogenesis and biofilm formation remain understudied. Although the Veillonella genus is broadly reported, species-level specification is lacking in sequencing datasets31,32. Therefore, we chose V. atypica, V. montpellierensis, and M. micronuciformis to represent Veillonellaceae family members present in the female genital tract to better understand their potential contributions to BV and the local microenvironment. The specific species and strains used in this study were isolated from the female genital tract of women with and without BV and are available from the BEI Resources, NIAID, NIH as part of the Human Microbiome Project.
The functional impact of V. atypica, V. montpellierensis, and M. micronuciformis on host response mechanisms in the human cervix, which can contribute to poor gynecologic and reproductive outcomes, has not been previously investigated. Therefore, we aimed to evaluate the immunometabolic contributions of V. atypica, V. montpellierensis, and M. micronuciformis in the cervical microenvironment to better understand the role of these species in BV and other sequalae. To achieve this, we used a robust and extensively characterized human three-dimensional (3-D) cervical epithelial cell model for the study of host-microbe interactions and infected the model with clinical isolates of Veillonellaceae family members, coupled with untargeted global metabolomics and immunoproteomics approaches. We chose to use a 3-D cervical cell model due to the clinical relevance of studying BV-associated bacteria in the context of the cervix as it relates to human papillomavirus (HPV), HIV, Chlamydia trachomatis, and Neisseria gonorrhoeae infections36,37,38,39. Additionally, M. micronuciformis has been isolated from women suffering from preterm birth40,41,42. Veillonella spp. have been implicated in secondary bacteremia in a pregnant woman, but the potential for Veillonella spp. to participate as opportunistic pathogens in the female reproductive tract is understudied43.
The 3-D cervical model is a robust system to study host-microbe interactions, as it accurately resembles ultrastructural features of the cervical tissue in vivo, such as apical and basal differentiation, tight junctions, microvilli, and more organotypic surfaces for the colonizing bacteria, and has been shown to recapitulate clinical findings15,44,45,46,47. Herein, we applied a reductionist approach and infected the 3-D cervical model with single bacterial species to determine the individual contributions of these bacteria to the cervicovaginal microbiome that are currently lacking. This knowledge is essential to inform future experiments that involve polymicrobial infections44,45,46,47.
Results
M. micronuciformis infections induce moderate cytotoxicity to cervical epithelial cells
First, we screened cervical epithelial monolayers for cytotoxicity following infection with V. atypica, V. montpellierensis, and M. micronuciformis at multiplicities of infection (MOIs) ranging from 2 to 400. We found that M. micronuciformis induced significant cytotoxicity at all tested MOIs (6.65%, 7.96%, and 13.63%) to cervical epithelial cell monolayers (P = 0.0291, P = 0.0041, and P < 0.0001 respectively); however, cervical epithelial cytotoxicity induced by M. micronuciformis infections was still modest at the highest MOI. V. montpellierensis infections induced cytotoxicity to cervical epithelial monolayers at only the highest MOI tested (6.50%, P = 0.0359). V. montpellierensis infections did not induce significant epithelial cytotoxicity at MOIs 2–4 and 20–40 (1.84%, 3.85%, respectively). Percentage cytotoxicity of V. atypica infections resulted in similar epithelial cytotoxicity at all tested MOIs (3.99%, 3.94%, and 6.23%) (Fig. 1A). Since Veillonella and Megasphaera spp. are predicted to harbor genes encoding lactate dehydrogenase (LDH)48,49, we also assessed the cytotoxicity using crystal violet staining (Supplementary Fig. 1). These results were consistent with LDH activity data with respect to cell viability measurements, indicating that potential bacterial LDH activity did not impact these data.
A LDH assay to quantify cytotoxicity in cervical epithelial monolayers. M. micronuciformis infections induced significant cytotoxicity to cervical epithelial monolayers at a range of MOIs from 2 to 400. V. montpellierensis infections induced significant cytotoxicity at the highest MOI only. Error bars designate standard deviation. Statistical differences were tested using one-way ANOVA with Dunnett’s adjustment for multiple comparisons, *P < 0.05, **P < 0.005 ****P < 0.0001. Scanning electron microscopy (SEM) images of B V. atypica, C V. montpellierensis, and D M. micronuciformis colonizing 3-D cervical epithelial cells. SEM images were pseudo-colored using Photoshop 19.0 CC (Adobe).
Next, we infected human 3-D cervical models with V. atypica, V. montpellierensis, and M. micronuciformis and confirmed colonization of the 3-D aggregates by SEM (Fig. 1B–D). Generally, V. atypica, V. montpellierensis, and M. micronuciformis sparsely colonized the 3-D cervical epithelial cells with occasional small clusters of bacteria co-localizing together on cervical cells. V. montpellierensis appeared to mostly colonize the surface of dead cells as visualized by SEM. M. micronuciformis also tended to sparsely cluster with other bacteria on cervical epithelial cell surfaces.
Infection with M. micronuciformis induces significantly elevated 3-D cervical cell pro-inflammatory responses while V. atypica significantly decreases the expression of specific epithelial barrier targets
Since BV is accompanied by mild genital inflammation, remodeling of the extracellular matrix, and cell lysis46,50, we examined the secretion of cytokines (IL-1α, IL-1β, IL-1RA, IL-6, and TNF-α), chemokines (fractalkine, IL-8, IP-10, MCP-1, MCP-3, MIP-1β, and RANTES), growth factors (PDGF-AA, TGF-α, and VEGF), apoptosis-related proteins (MIF, TRAIL, sFasL), mucins (CEA and CA125) and matrix metalloproteinases (MMP-1, MMP-7, MMP-9, MMP-10) in the 3-D cervical epithelial cell models infected with V. atypica, V. montpellierensis or M. micronuciformis at MOI 20–40 for 24 h. We analyzed 3-D cervical cell culture supernatants using cytometric bead arrays to quantify the host response to infection with these bacteria. V. atypica induced significant elevation of sFasL (P = 0.0202), IL-1RA (P = 0.0498), TNF-α (P = 0.0152), and significantly decreased secretion of PDGF-AA (P = 0.0083), VEGF (P = 0.0245), and MMP-10 (P = 0.0064). Infection with V. montpellierensis induced significant elevation of sFasL (P = 0.0250) and IL-1α (P = 0.0137), IL-1RA (P = 0.0095), TNF-α (P < 0.0001), IL-1β (P = 0.0470). M. micronuciformis significantly induced secretion of sFasL (P = 0.0065), TNF-α (P = 0.0056), IL-1β (P = 0.0012), IL-6 (P = 0.0004), fractalkine (P < 0.0001), and IL-8 (P = 0.0012) (Fig. 2A, Supplementary Figs 2–4).
A Hierarchical clustering analysis (HCA) of immunoproteomics data from human 3-D cervical epithelial cells infected with V. atypica, V. montpellierensis, or M. micronuciformis under anaerobic conditions for 24 h. Statistical significance was determined using one-way ANOVA with Dunnett’s adjustment for multiple comparisons relative to PBS; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Color labels for each bacterial infection are observed to the right of the HCA and are as follows: PBS (gray), V. atypica (blue), V. montpellierensis (green), and M. micronuciformis (red). Protein targets (bottom of HCA) were color-coded in order to visualize clustering patterns of the protein targets: apoptotic factors (pink), epithelial barrier targets (yellow), growth and proliferation factors (orange), chemokines (dark blue), and cytokine (light blue). Individual concentrations can be visualized on graphs in the supplementary information (Supplementary Figs. 2–4).
We utilized hierarchical clustering analysis for evaluating immune response profiles for each bacterial infection. V. atypica and V. montpellierensis exhibited similar immunoproteomic profiles and clustered separately from M. micronuciformis or PBS controls (Fig. 2A). Overall, M. micronuciformis induced the secretion of pro-inflammatory cytokines and chemokines to a greater extent compared to V. atypica and V. montpellierensis and PBS controls. Infection with V. atypica resulted in a decrease in the concentration of PDGF-AA, VEGF, and MMP-10.
Untargeted global metabolomics analysis revealed similar metabolic profiles between V. atypica and V. montpellierensis that are distinct from M. micronuciformis
We next sought to define how V. atypica, V. montpellierensis, and M. micronuciformis alter the metabolomic landscape of 3-D cervical cell culture supernatants using high-throughput global untargeted metabolomics. Principal component analysis (PCA) of global metabolomic profiles (Fig. 3A) revealed that V. atypica and V. montpellierensis metabolomes cluster together, whereas M. micronuciformis and PBS controls tended to cluster together and separately from both Veillonella groups. Relative to PBS controls, PC1 (36.8% of explained variance) scores were significantly different between V. atypica and V. montpellierensis (P < 0.0001). No significant differences were found between PBS controls and any strain tested with respect to PC2 (19.2% of explained variance) scores.
Global metabolic analysis of cell culture supernatants from human 3-D cervical epithelial cells infected with V. atypica, V. montpellierensis, or M. micronuciformis under anaerobic conditions for 24 h. A Principal component analysis (PCA) shading indicates the 95% confidence interval and B Pearson’s correlation analysis of metabolomics data displays how sample metabolic profiles relate to each other. Blue and red coloring indicate negative and positive correlations between samples, respectively. C Pie charts of significantly changed metabolites. The percentage of metabolites that were approached significance (P < 0.1) within each super pathway are labeled next to each slice (the total number of significantly altered metabolites for V. atypica, V. montpellierensis, and M. micronuciformis were 69, 98, 63 respectively). PCA significance (A) was tested by one-way ANOVA and Dunnett’s adjustment for multiple comparisons relative to PBS; ****P < 0.0001. The significance of differences between metabolite compositions (C) was tested using a chi-squared test of trends; **P < 0.01.
We then applied non-parametric correlation analyses (Fig. 3B) to visualize the clustering of biological replicates. We observed that metabolomic profiles of V. atypica and V. montpellierensis correlated well with each other; however, the cluster analysis failed to group individual replicates of the same species, therefore suggesting highly similar metabolic profiles among the two Veillonella spp. While in the same parent cluster, the metabolomic profiles for M. micronuciformis and PBS controls mostly separated from each other. PBS controls correlated well between individual replicates, with the exception of one infection replicate with M. micronuciformis. Overall, the correlation analysis supported the PCA results.
We further investigated differences in metabolic profiles of V. atypica, V. montpellierensis, and M. micronuciformis infections by comparing the super-pathways of significantly changed metabolites relative to PBS controls (Fig. 3C). The composition of metabolic super-pathways for M. micronuciformis was significantly different from that of V. atypica (P = 0.0016) and V. montpellierensis (P < 0.0027). The super-pathways that were most different between V. atypica, V. montpellierensis, and M. micronuciformis were lipid (8.70%, 16.33%, and 38.1% respectively) and amino acid (47.83%, 52.04%, and 31.75% respectively).
Veillonella spp. and M. micronuciformis infections result in enrichment in amino acids and lipids, respectively
Next, we performed metabolite pathway enrichment analysis using MetaboAnalyst (Fig. 3C). Metabolomic profiles from infection with V. atypica and V. montpellierensis were significantly enriched in metabolic pathways corresponding to arginine and proline (P = 0.0151 and P = 0.0076, respectively), butanoate (P = 0.0032 and P = 0.0060, respectively), cysteine and methionine (P = 0.0041 and P = 0.0014, respectively), histidine (P = 0.0106 and P = 0.0089, respectively) and glutathione (P = 0.0117 and P = 0.0061, respectively) metabolism (Fig. 4D). Infection with V. montpellierensis also significantly enriched four additional pathways corresponding to amino sugar and nucleotide sugar (P = 0.0199), riboflavin (P = 0.0425), tryptophan (P = 0.0394), and glycerolipid (P = 0.0497) metabolism.
Metabolite pathway enrichment analysis of human 3-D cervical epithelial cells infected with A V. atypica (Supplementary Fig. 3), B V. montpellierensis (Supplementary Fig. 4), and C M. micronuciformis (Supplementary Fig. 5), under anaerobic conditions for 24 h. D Comparison of significantly enriched metabolic pathways between infections. Color bars indicate significance, yellow represents pathways that are significantly enriched (P < 0.05), green indicates pathways that approach statistical significance (0.05 < P < 0.1), and blue indicates pathways that were not significantly enriched (P > 0.1). The super pathway that a particular sub-pathway belongs to is indicated with colored circles.
Infection with M. micronuciformis modulated five significantly enriched pathways, including tyrosine metabolism (P = 0.0493), sulfate metabolism (P = 0.0309), steroid biosynthesis (P = 0.0159), steroid hormone biosynthesis (P = 0.0189), and primary bile acid synthesis (P = 0.01520). These pathways were unique to infection with M. micronuciformis relative to V. atypica or V. montpellierensis infections (Supplementary Figure 5).
We found variations in lipid metabolism both in our metabolite composition analysis (Fig. 3C) and enrichment analysis (Fig. 4D) for V. montpellierensis and M. micronuciformis infections. Significantly enriched pathways corresponding to lipid metabolism included glycerolipid metabolism and steroid and steroid hormone biosynthesis as modulated by V. montpellierensis and M. micronuciformis, respectively. Metabolic pathways corresponding to lipid metabolism were not significantly enriched by infection with V. atypica, therefore suggesting V. montpellierensis and M. micronuciformis influence species-specific lipid metabolic products following infection of 3-D cervical cells.
V. atypica and V. montpellierensis infections resulted in significant elevation of polyamines and depletion of lactate, whereas glycerolipids were significantly elevated with M. micronuciformis infections
Finally, we assessed specific metabolites that were significantly elevated or depleted in response to V. atypica, V. montpellierensis, and M. micronuciformis infections relative to PBS controls. Culture supernatants from human 3-D cervical cells infected with V. atypica and V. montpellierensis significantly accumulated polyamines, such as cadaverine (P = 0.0159 and P = 0.0037), putrescine (P = 0.0155 and P = 0.00485), agmatine (P = 0.0444 and P = 0.0170), and histamine (P = 0.0331 and P = 0.0452) (Fig. 5B). Additionally, V. montpellierensis infections induced accumulation of spermidine (P = 0.0363) and N-acetyl-cadaverine (P = 0.00207). Metabolites involved in polyamine production were significantly depleted by infection with V. atypica and V. montpellierensis, including argininosuccinate (P = 0.0319 and P = 0.0179), ornithine (P = 0.0108 and P = 0.00140), and 5-methylthioadenosine (P = 0.0216 and P = 0.0266). The cervicovaginal pH-lowering metabolite, lactate, was also significantly depleted by infection with V. atypica and V. montpellierensis (P = 0.0162 and P < 0.0001, respectively).
Human 3-D cervical epithelial cells were infected with V. atypica, V. montpellierensis, or M. micronuciformis under anaerobic conditions for 24 h. A Volcano plots of V. atypica, V. montpellierensis and M. micronuciformis metabolic profiles. The x axis represents the log2 fold change values of metabolite scaled intensity values between the infectious treatment and PBS control. The y axis represents the −log10(P) value of differential metabolite abundance between infectious treatment and PBS controls (paired two-tailed Student’s t-test). B Significantly different metabolites (P < 0.05) with a fold-change < −2 or >2 are colored by super pathway. Symbols indicate how the metabolite may impact BV pathogenesis. See Supplementary Data 1 for specific P-values, q-values, and mean fold changes of all measured metabolites.
Culture supernatants from 3-D cervical cells infected with M. micronuciformis accumulated glycolipids and sphingolipids (Fig. 5 and Supplementary Data 1). The short-chain fatty acid (SCFA) 2-hydroxybutyrate/2-hydroxyisobutyrate was also significantly elevated by infection with V. atypica, V. montpellierensis, and M. micronuciformis (P = 0.0344, P = 0.0006, and P = 0.0253, respectively). Specific glycerophospholipid and sphingolipid species were significantly elevated following M. micronuciformis infections including: 1,2-dipalmitoyl-GPC (16:0/16:0) (P = 0.0188), N-palmitoyl-sphingosine (d18:1/16:0) (P = 0.0363), cholesterol (P = 0.0465) and 1-stearoyl-2-oleoyl-GPC (18:0/18:1) (P = 0.0470). 1-(1-enyl-palmitoyl)-2-oleoyl-GPE (P-16:0/18:1) was significantly elevated by infection with V. atypica, V. montpellierensis, and M. micronuciformis (P = 0.0438, P = 0.0292, and P = 0.0426, respectively). In V. montpellierensis and M. micronuciformis infections, 1-palmitoyl-2-oleoyl-GPE (16:0/18:1) was significantly elevated (P = 0.00969 and P = 0.0209, respectively). Two glycerolipids were significantly elevated by infection with V. montpellierensis: glycerol-3-phosphate (P = 0.0405) and 1-stearoyl-2-oleoyl-GPE (18:0/18:1) (P = 0.0491). Overall, M. micronuciformis infections significantly elevated the glycerophospholipids and sphingolipids to a greater magnitude relative to V. atypica and V. montpellierensis infections.
In addition, 2-hydroxyglutarate (an oncometabolite) was significantly elevated during infection with V. atypica and V. montpellierensis (P = 0.0308 and P = 0.0052, respectively), with V. atypica infections (P = 0.0268), and betaine was altered with V. montpellierensis infections (P = 0.0102). Urate (an HPV-associated metabolite)51 was significantly increased with V. atypica infections (P = 0.0244).
Overall, infection of 3-D cervical epithelial cells with V. atypica or V. montpellierensis induced significant elevation of metabolites associated with a higher cervicovaginal pH and amine odor, combined with depletion of metabolites involved with polyamine biosynthesis. M. micronuciformis infections significantly elevated glycerolipids and sphingolipids that are potentially indicative of cervical epithelial cell cytotoxicity (Fig. 5).
Discussion
Members of the Veillonellaceae family, V. atypica, V. montpellierensis, and M. micronuciformis have been isolated from women with BV and healthy women2,16,17,31,32,34,40; however, the mechanisms employed by these bacteria that relate to BV pathogenesis and other gynecologic and obstetric sequalae are unknown. In this study, we aimed to elucidate the metabolic and inflammatory contributions of V. atypica, V. montpellierensis, and M. micronuciformis to reveal the function of these organisms in the cervical microenvironment.
To our knowledge, colonization of human organotypic genital epithelial cells by V. atypica, V. montpellierensis, or M. micronuciformis in vitro has not been previously demonstrated and visualized. Using our human 3-D cervical epithelial cell model, we demonstrated that V. atypica, V. montpellierensis, and M. micronuciformis are capable of colonizing cervical epithelial cell surfaces (Fig. 1B–D). Our human 3-D cervical epithelial cell model expresses mucins and TLRs and other physiological relevant features of parental human cervical tissue that may enhance colonization of bacteria compared to traditional 2-D monolayer cultures46,52.
The cytotoxic potential of Veillonellaceae members in the cervical epithelium has not been thoroughly explored. One study tested an unnamed Veillonella spp. on vaginal epithelial cell monolayers and found no evidence for cell death, though this was not quantified53. Our data suggest that M. micronuciformis induces moderate cytotoxicity, whereas V. atypica and V. montpellierensis do not significantly contribute to cervical epithelial cell death (Fig. 1). In this capacity, M. micronuciformis may therefore contribute to the breakdown of epithelial barrier function, in accordance with other BV-associated bacteria4.
To our knowledge, the pro-inflammatory host response to cervical infection with M. micronuciformis has not been previously examined in vitro, though another species under the Megasphaera genus (Megasphaera elsdenii) has been investigated54. In this study, dendritic cells elevated secretion of IL-1β, IL-6, IL-8, IL-12p40, and TNF-α in response to infection with M. elsdenii54. Additionally, M. micronuciformis was associated with genital inflammation as measured from cervicovaginal fluid in a cohort of South African adolescent females55. However, no studies have identified the immune response to V. atypica and V. montpellierensis in the cervicovaginal context. Our results suggest that M. micronuciformis induced a robust human 3-D cervical epithelial cell pro-inflammatory response marked by upregulated secretion of pro-inflammatory cytokines and chemokines, whereas both V. atypica and V. montpellierensis induced relatively fewer pro-inflammatory mediators (Fig. 2A). We hypothesize that M. micronuciformis is more pro-inflammatory than V. atypica or V. montpellierensis in the cervicovaginal environment, which is in the accordance with the literature, although more studies are required that investigate Veillonella spp. in the cervicovaginal environment.
SCFA production is a metabolic hallmark of BV as demonstrated in clinical and in vitro studies56,57,58 and have been linked to increased secretion of pro-inflammatory mediators59. We observed a higher accumulation of N-palmitoyl-sphingosine in response to infection with M. micronuciformis compared to V. atypica or V. montpellierensis, and is associated with inflammation60. Veillonella spp. and M. micronuciformis also induced upregulated secretion of several pro-inflammatory mediators from the 3-D cervical model, which is consistent with the literature and SCFA elevation59 and could indicate that SCFAs are both a hallmark of BV and indicators of cervical inflammation.
Histamine, a key metabolite involved in local host responses, was significantly accumulated by infection with V. atypica and V. montpellierensis. Other studies have found that histamine can decrease the expression of tight junctional proteins (ZO-1 in nasal epithelial cells and E-cadherin in epithelial pulmonary cells)61. Therefore, an increase in histamine may increase epithelial permeability, although this would need to be tested in the context of the cervicovaginal epithelium to confirm the role of histamine in barrier function within the cervicovaginal mucosa.
Our metabolomics analyses revealed changes in the lipid metabolism following infections with Veillonellaceae family members. Infection with M. micronuciformis induced significant accumulation in glycerolipids and sphingolipids (Fig. 5). We also observed a significant accumulation in glycerolipids and cholesterol during human 3-D cervical cell infections with V. atypica, V. montpellierensis, and M. micronuciformis. To date, there are few studies that have reported an association with elevated glycerolipids and cell death, but sphingolipids have been related to cell death62. In our previous clinical study, elevation in the relative abundance of sphingomyelin and 1-stearoyl-2-docosahexaenoyl-GPC correlated with Lactobacillus depletion and genital inflammation51. However, additional studies are needed in order to correlate elevated glycerolipids with cell death.
Early reports that dissect the metabolic potential of Veillonella49,63,64,65,66 revealed that Veillonella spp. utilize lactate as a sole energy source49,64,67. Health-associated Lactobacillus spp. secrete copious amounts of lactic acid into the cervicovaginal milieu14,68,69 and lactic acid is depleted in severe cases of BV3. It is therefore possible that V. atypica and V. montpellierensis elicit their pathogenicity in the early stages of BV when lactate is still available. Our data support lactate utilization by V. atypica and V. montpellierensis (Fig. 5). Since current literature indicates lactate consumption by other Megasphaera spp.70, our finding could be explained by suggesting that lactate serves as a non-primary energy source for Megasphaera. Importantly, we have shown that Veillonella spp. consume lactate (key metabolite associated with cervicovaginal health) and may contribute to changes in the cervicovaginal pH71. Indeed, in our previous clinical study investigating the vaginal microbiome in women across cervical neoplasia, V. montpellierensis and other Veillonella spp. were enriched in women with abnormal cervicovaginal pH33.
Elevation of cervicovaginal polyamines is a metabolic hallmark of BV and contributes to increased pH and amine odor, both of which are key clinical symptoms of BV72. Polyamine synthesis by Veillonella has been documented in the oral microbiome and contributes to malodor73. Additionally, one clinical study demonstrated that M. micronuciformis was associated with elevated cervicovaginal putrescine14. Our data revealed that in the context of our human 3-D cervical epithelial cell model, both V. atypica and V. montpellierensis modulate a variety of polyamines, including cadaverine, putrescine, histamine, and agmatine, whereas polyamines were not significantly elevated by M. micronuciformis infection (Fig. 5). Additionally, in previous clinical studies the metabolite 5′-methylthioadenosine (MTA), a by-product of polyamine synthesis, has been found to be depleted in women harboring non-Lactobacillus dominant microbiomes72,74,75. In our current study, MTA was also depleted in V. atypica and V. montpellierensis infections. An earlier study14 provided clinical correlations for putrescine production by Megasphaera in a vaginal context, which is not in accordance with our data; however, the next-generation sequencing method employed was unable to identify Megasphaera at the species level. It is possible that other Megasphaera spp. present in the cervicovaginal microbiome may exert differential metabolic capabilities that influenced the observed elevation of putrescine in this study14. However, both the increase in polyamine synthesis and depletion of MTA observed in infections with V. atypica and V. montpellierensis suggest that these microorganisms may significantly impact polyamine synthesis in vivo and influence metabolic hallmarks of BV (e.g., putrescine and cadaverine).
Our data also revealed arginine depletion following M. micronuciformis infection. Nitric oxide, agmatine, and ornithine are by-products of arginine catabolism5,76. Considering neither agmatine nor ornithine was significantly altered in our analysis, perhaps the observed depletion of arginine was a result of nitric oxide production by 3-D cervical cells. Nitric oxide has been implicated as an important toxic defense molecule against pathogens77 and has a role in inflammation and the immune response, further indicating pro-inflammatory properties of M. micronuciformis in the cervicovaginal microenvironment78.
In summary, lactate depletion and polyamine production observed in our 3-D human cervical cell model suggest that Veillonella spp. contribute to elevated cervicovaginal pH via consumption of a key metabolite linked to gynecologic health (Fig. 6). We found that M. micronuciformis exhibited greater pro-inflammatory properties compared to V. atypica and V. montpellierensis. M. micronuciformis infections also resulted in higher levels of epithelial cytotoxicity and a higher enrichment in SCFAs that can also impact the production of pro-inflammatory cytokines and chemokines or cell death (Fig. 6). In line with our working hypothesis, our results provide evidence that Veillonellaceae family members alter the local microenvironment in a distinctive fashion. As putative primary colonizers, Veillonella spp. may create a favorable environment for secondary colonizers. On the other hand, M. micronuciformis may participate as a putative secondary colonizer that induces inflammation in the cervicovaginal tract and manifest as symptoms and clinical presentation of BV, as well as other gynecologic and reproductive sequalae (Fig. 6).
Summary of immunoproteomics and metabolomics analyses of 3-D cervical cells infected with V. atypica, V. montpellierensis or M. micronuciformis. A Lactate was depleted and polyamines (putrescine and cadaverine) are significantly accumulated in cell culture supernatants of 3-D cervical cells infected with either V. atypica or V. montpellierensis. Lactate (metabolic hallmark of cervicovaginal health) depletion and elevated polyamine levels may correspond with an increase in pH, a clinical hallmark of BV. B The SCFA 2-hydroxybutyrate was significantly accumulated in the cell culture supernatants of 3-D cervical cells infected with M. micronuciformis. In addition, several pro-inflammatory mediators were elevated following infection with M. micronuciformis, including IL-1β, IL-6, IL-8, Fractalkine, sFasL, TNF-α. Finally, cytotoxicity/viability analyses revealed that M. micronuciformis infection resulted in moderate cervical epithelial cell death, as well as enrichment of sphingosine that could suggest the induction of cell death. Overall, M. micronuciformis may promote a pro-inflammatory immune profile within the cervical microenvironment, and cause cervical epithelial cell damage, compared to both Veillonella spp.
All experimental model systems have their own strengths and weaknesses79. We acknowledge the polymicrobial nature of BV and inter-bacterial and host-microbe interactions that participate in the development and progression of the disease. In this study, we used a reductionist approach and performed mono-infections using our robust 3-D cervical cell model to dissect the individual contributions of specific Veillonellaceae members. This approach is required to first understand individual pathologic contributions from these understudied bacteria. This data will provide the foundations for future studies that utilize polymicrobial cocktails that better represent the complexity and microbe-microbe interactions present in the microbiome. Finally, our untargeted metabolomics approach is beneficial since it allows us to examine metabolic changes on a global scale. This technique is limited, however, by the relativistic nature of metabolite measurements and is therefore unable to provide absolute metabolite quantification. Future studies employing targeted metabolomics will help to directly compare concentrations of key cervicovaginal metabolites within in vivo samples and in vitro models.
Future studies on Veillonellaceae members in the context of the cervicovaginal epithelium should focus on the temporal colonization patterns of BV-associated organisms, as this will provide insights into the potential contribution to biofilm formation for these microorganisms. In addition, studies of polymicrobial “cocktails” that combine Veillonellaceae members with other vaginal bacterial isolates will provide additional insights into bacteria-bacteria interactions in the context of the cervicovaginal microenvironment. In conclusion, the unique mechanisms and individual contributions of Veillonellaceae family members defined herein may promote our understanding of biofilm formation characteristic of BV and the underlying mechanisms that contribute to adverse obstetric/gynecologic health outcomes related to BV.
Methods
Human cervical epithelial cell monolayer and three-dimensional human cervical cell model culture
Human cervical epithelial cells (A2EN) were grown as monolayers with keratinocyte serum-free media (Fischer Scientific) supplemented with endothelial growth factor (5 ng/ml), bovine pituitary extract (50 µg/ml), and Primocin (100 µg/ml) in the humidified atmosphere of 5% CO2 at 37 °C46. Primocin was not included in the media used in the bacterial infection assays outlined below. For 2-D monolayer cultures, cells were seeded into culture-treated 24-well plates to a cell density of ~2 × 105 cells/ml. Prior to seeding, cells were enumerated using the Countess automated cell counter (Invitrogen) and trypan blue exclusion, which were used for cytotoxicity assays.
To generate the 3-D cervical cell model, human cervical epithelial A2EN cells were grown on Cytodex-3® collagen-coated dextran microcarrier beads (Sigma-Aldrich) in a rotating wall vessel bioreactor (Synthecon), as previously described15,45,46,80. Following the 28-day differentiation period, aggregates were seeded into 24-well culture-treated plates at the density of 1 × 105–5 × 105 cells/ml and used for downstream analyses.
Bacterial strains and growth conditions
V. atypica strain CMW7756B and V. montpellierensis strain DNF00314 were cultured on brain heart infusion agar (Becton Dickinson) supplemented with 5% (v/v) defibrinated sheep blood (Quad Five) and 0.56 mg/l of sodium d-lactate (Sigma-Aldrich). M. micronuciformis strain DNF00954 was cultured on tryptic soy agar (Becton Dickinson) supplemented with 5% (v/v) defibrinated sheep blood (Quad Five). Bacteria were grown at 37 °C under anaerobic conditions generated using AnaeroPack sachets and anaerobic environmental chambers (Thermo Scientific). All strains were obtained from Biodefense and Emerging Infections Research Resources Repository (https://www.beiresources.org). M. micronuciformis strain DNF00954 and V. montpellierensis strain DNF00314 were isolated from women with BV, whereas V. atypica strain CMW7756B was isolated from the genital tract of a pregnant woman.
Bacterial infection of human cervical epithelial cells
Prior to the infection, bacteria were grown for 16–18 h on appropriate agar plates, resuspended in sterile Dulbecco’s phosphate-buffered saline (PBS), and adjusted to an optical density at 600 nm (OD600) 0.5. The viability of bacteria was confirmed using the standard plating assay. The adjusted bacterial cell suspensions were serially diluted in PBS, plated on appropriate agar media, and incubated for 96 h at 37 °C under anaerobic conditions for enumeration of colony-forming units (CFU). The bacterial suspensions of OD600 0.5 corresponded to bacterial densities of approximately 1–2 × 108 CFU/ml. Human cervical cell 2-D monolayers used for cytotoxicity assays were infected with a range of bacterial suspensions equivalent to OD600 of 0.001, 0.01, and 0.1 per 1× 105 cervical cells/ml, which corresponded to multiplicities of infection (MOI) of 2–4, 20–40, and 200–400. Infected cervical cells were incubated for 24 h at 37 °C under anaerobic conditions. In a preliminary experiment, after 24 h incubation with cervical cells, the viability of bacteria was confirmed and did not change more than 1 log when compared to the initial inocula. The 3-D cervical epithelial cells were infected with the bacterial resuspensions at an MOI of 20–40 for 24 h at 37 °C under anaerobic conditions, as described above.
Lactate dehydrogenase (LDH) assay to assess cytotoxicity
The LDH assay (Invitrogen, ThermoFisher Scientific) was performed according to the manufacturer’s guidelines. LDH activity was measured from cell culture supernatants by recording absorbance values at 490 and 680 nm. Spontaneous LDH activity was measured from PBS-treated cells and maximum LDH activity was measured from lysed cells. Percentage cytotoxicity was calculated by using the following formula: (bacteria-infected LDH activity − spontaneous LDH activity)/(the maximum LDH activity − spontaneous LDH activity) × 100.
Scanning electron microscopy
Three-dimensional cervical epithelial cells were infected with each bacterial species at an MOI of 200–400 for 4 h at 37 °C under anaerobic conditions as described above. Samples were fixed and processed for scanning electron microscopy (SEM) as previously described80,81. Samples were imaged using a JSM-6300 JEOL scanning electron microscope and images were obtained with the IXRF model 500 digital processor (IXRF systems). SEM images were pseudo-colored using Photoshop 19.0 CC (Adobe).
Immunoproteomic analysis of cell culture supernatants
Cell culture supernatants collected from at least three independent replicates of 3-D human cervical epithelial cells infected with bacteria as described above were used to quantify concentrations of 15 protein targets. PBS-treated cells served as negative controls. Cytometric bead arrays were performed using customized MILLIPLEX Multianalyte Profiling (MAP) Human Cytokine and Chemokine Panel 1, Human Circulating Cancer Panel 2, and Human Matrix Metalloproteinases Panel 2 (Millipore) in accordance with manufacturer guidelines. The tested targets included fractalkine, interleukin (IL)-1α, IL-1β, IL-6, IL-8, interferon-γ-induced protein-10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), MCP-3, macrophage inflammatory protein-1β (MIP-1β), platelet-derived growth factor-AA (PDGF-AA), regulated on activation, normal T-cell expressed and secreted (RANTES), transforming growth factor-α (TGF-α), tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF), macrophage migration inhibitory factor (MIF), tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), soluble Fas ligand (sFasL), carcinoembryonic antigen (CEA), cancer antigen 125/mucin 16 (CA125), matrix metalloproteinase-1 (MMP-1), MMP-7, MMP-9, and MMP-10. Data was collected and analyzed using a Bio-Plex® 200 (Bio-Rad) platform and Bio-Plex® Manager (5.0) software (Bio-Rad). A 5-parameter logistic regression curve fit was used to determine concentrations. All reported values for MCP-1, MCP-3, MIP-1β, and CA125 were below the minimum detectable concentration and were therefore not included in the further analyses. All samples were assayed in duplicate.
Untargeted global metabolomics analysis of cell culture supernatants
Cell culture supernatants collected from three independent 3-D cervical cell aggregate batches for both Veillonella strains and four independent batches for M. micronuciformis infections were sent to Metabolon Inc. (Durham, NC) for untargeted global metabolomics analysis. Metabolites were resolved on a Waters ACQUITY ultra-performance liquid chromatography (UPLC) and a Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer interfaced with a heated electrospray ionization (HESI-II) source and Orbitrap mass analyzer operated at 35,000 mass resolution. The sample extract was dried then reconstituted in solvents compatible to each of the four methods. Each reconstitution solvent contained a series of standards at fixed concentrations to ensure injection and chromatographic consistency. One aliquot was analyzed using acidic positive ion conditions, chromatographically optimized for more hydrophilic compounds. In this method, the extract was gradient eluted from a C18 column (Waters UPLC BEH C18-2.1 × 100 mm, 1.7 µm) using water and methanol, containing 0.05% perfluoropentanoic acid (PFPA) and 0.1% formic acid (FA). Another aliquot was also analyzed using acidic positive ion conditions; however, it was chromatographically optimized for more hydrophobic compounds. In this method, the extract was gradient eluted from the same aforementioned C18 column using methanol, acetonitrile, water, 0.05% PFPA, and 0.01% FA and was operated at an overall higher organic content. Another aliquot was analyzed using basic negative ion optimized conditions using a separate dedicated C18 column. The basic extracts were gradient eluted from the column using methanol and water, however with 6.5 mM ammonium bicarbonate at pH 8. The fourth aliquot was analyzed via negative ionization following elution from a HILIC column (Waters UPLC BEH Amide 2.1 × 150 mm, 1.7 µm) using a gradient consisting of water and acetonitrile with 10 mM ammonium formate, pH 10.8. The MS analysis alternated between MS and data-dependent MSn scans using dynamic exclusion. The scan range varied slighted between methods but covered 70–1000 m/z.
The bioinformatics system consisted of four major components, the Laboratory Information Management System (LIMS), the data extraction and peak-identification software, data processing tools for QC and compound identification, and a collection of information interpretation and visualization tools for use by data analysts. The hardware and software foundations for these informatics components were the LAN backbone, and a database server running Oracle 10.2.0.1 Enterprise Edition. Peaks were quantified using area-under-the-curve, which allows determining relative intensity of compounds among tested samples, but not the absolute concentrations.
Statistical analyses
All infections and assays were performed as at least three independent replicates. One-way ANOVA with Dunnett’s adjustment for multiple comparisons was used to statistically analyze the immunoproteomics data. One-way ANOVA with Bonferroni post-hoc tests were used to test for significant differences in LDH data. Differences in metabolite pathway composition were determined by chi-squared analysis. One-way ANOVA analysis was performed using Prism v8 software (GraphPad). Hierarchical clustering analysis and heatmap visualization were performed using ClustVis82. Metabolite intensity values were median-scaled and log-transformed prior to performing two-tailed paired Student’s t-tests (infection vs. PBS control) using the R rstatix package. Metaboanalyst 4.083 was used for principal component analysis (PCA), Pearson’s correlation analysis, and metabolite pathway enrichment analysis. P-values of <0.05 were considered significant in all analyses. Metabolomics results were corrected for multiple testing using the false discovery rate (FDR) and q-values were reported. All error bars represent standard deviation.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The authors declare that the data supporting the findings of this study are available within the paper and its Supplementary Information files. Additional data are available from the corresponding author upon reasonable request.
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Acknowledgements
We thank David Lowry at Arizona State University for his aid in SEM sample preparation and imaging. In addition, we are thankful to Dr. Zehra Esra Ilhan for critically reviewing the manuscript. Also, we would like to acknowledge the Biodefense and Emerging Infections Research Resources Repository (https://www.beiresources.org), NIAID, NIH as a part of the Human Microbiome Project for supplying bacterial isolates for this study; V. atypica strain CMW7756B, V. montpellierensis strain DNF00314 and M. micronuciformis strain DNF00954. Funding for this project was provided by the NIH National Cancer Institute and a supplement from the Office of Research for Women’s Health (3P30CA023074-39S3) and the Flinn Foundation Grant# 2244.
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M.M.H.-K. conceived of the hypothesis and experimental design in collaboration with J.D.M. and P.Ł. M.E.S. carried out 3-D cervical cell infections used in immunometabolomics analysis, wrote the first draft of the manuscript, drafted and edited the figures, and revised the manuscript. J.D.M. obtained SEM images of infected 3-D cervical cells and assisted in statistical analysis. C.G. performed LDH and crystal violet assays, analysis of LDH data, and presentation of LDH figures, as well as revision of the manuscript. M.M.H-K., J.D.M., and P.Ł. provided guidance and feedback on writing, figures and tables, and read and revised the manuscript. M.M.H.-K. provided supervision of the experimental and writing processes. All authors read, revised, and approved the final version of the manuscript.
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Salliss, M.E., Maarsingh, J.D., Garza, C. et al. Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model. npj Biofilms Microbiomes 7, 57 (2021). https://doi.org/10.1038/s41522-021-00229-0
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DOI: https://doi.org/10.1038/s41522-021-00229-0
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