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Sandwich-type electrochemical immunosensing of hypopharyngeal carcinoma biomarker carcinoembryonic antigen based on N-doped hollow mesoporous nanocarbon spheres/gold hybrids as sensing platform and gold/ferrocene as signal amplifier

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Abstract

In the present work, a highly sensitive sandwich-type electrochemical immunosensor of carcinoembryonic antigen (CEA) was developed by preparing N-doped hollow mesoporous nanocarbon spheres/gold hybrids (NHMN/Au) hybridsas sensing platformand Au/ferrocene (Au/Fc) as signal amplifiers. The large surface area and high conductivity as well as good biocompatibility of NHMN/Au can increase the loading of primary antibody (Ab1) and accelerate the electron transport rate of the electrode surface, while Au can carry immobilized secondary antibodies (Ab2) and Fc derivative (Fc–SH).By using Fc–SH as response probe, the experiments show that the peak current of probe could increase after occurring the specific recognition of Ab1-CEA-Ab2, thus a novel sandwich-type immunosensor of CEA was developed. Finally, the proposed method for CEA detection was applied in human serum and the obtained results are satisfactory, indicating the developed method has important clinical applications for CEA determination.

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Acknowledgements

This work was financially supported by Fujian natural science foundation project (2017J01271) and Fujian health education joint project (WKJ2016-2-36).

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Author notes

  1. Chaohui Zheng and Jing Chen contributed equally to this work.

Authors and Affiliations

  1. Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China

    Chaohui Zheng, Yizheng Zhang, Jiao Zhou, Xiaofang Chen & Yuming Hong

  2. Department of Pharmacology, College of Pharmacy, Quanzhou Medical College, Quanzhou, 362010, China

    Jing Chen

  3. Department of Clinical Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian, China

    Shuang Zhou & Lixing Wang

  4. College of Chemical Engineering and Materials Science, Quanzhou Normal University, Fujian Province, Quanzhou, 362002, China

    Dapeng Yang

Authors
  1. Chaohui Zheng
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  4. Shuang Zhou
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  7. Xiaofang Chen
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  9. Yuming Hong
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Correspondence to Yuming Hong.

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Advanced Publication Released Online by J-STAGE 5-Mar-2021.

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Zheng, C., Chen, J., Zhang, Y. et al. Sandwich-type electrochemical immunosensing of hypopharyngeal carcinoma biomarker carcinoembryonic antigen based on N-doped hollow mesoporous nanocarbon spheres/gold hybrids as sensing platform and gold/ferrocene as signal amplifier. ANAL. SCI. 39, 5–11 (2023). https://doi.org/10.1007/s44211-022-00223-2

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