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A sandwich-type electrochemical immunosensor for CYFRA 21–1 based on probe-confined in PtPd/polydopamine/hollow carbon spheres coupled with dendritic Au@Rh nanocrystals

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Abstract

A signal-on sandwich-like electrochemical immunosensor was built for determination of cytokeratin 19 fragments 21–1 (CYFRA 21–1) in non-small cell lung cancer (NSCLC) by confining electroactive dye (e.g., methylene blue, MB) as a probe for amplifying signals. Specifically, core–shell gold@rhodium dendritic nanocrystals (Au@Rh DNCs) behaved as a substrate for primary antibody and accelerate interfacial electron transfer. Besides, hollow carbon spheres (HCSs) were subsequently modified with polydopamine (PDA) and PtPd nanoparticles for sequential integration of the secondary antibody and confinement of MB as a label, termed as MB/PtPd/PDA/HCSs for clarity. The built sensors showed a broad linear range (100 fg mL−1 ~ 100 ng mL−1) for detection of CYFRA 21–1 with an ultra-low detection limit (31.72 fg mL−1, S/N = 3), coupled with satisfactory performance in human serum samples. This work can be explored for assays of other proteins and provides some constructive insights for early and accurate diagnosis of NSCLC.

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Funding

This research was supported by the National Natural Science Foundation of China (No. 32070397).

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Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China

    Yi-Ge Feng, Jia-Wen He, Di-Nan Chen, Lu-Yao Jiang, Ai-Jun Wang & Jiu-Ju Feng

  2. School of Public Health, Nantong University, Nantong, 226019, China

    Ning Bao

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  1. Yi-Ge Feng
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  2. Jia-Wen He
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Feng, YG., He, JW., Chen, DN. et al. A sandwich-type electrochemical immunosensor for CYFRA 21–1 based on probe-confined in PtPd/polydopamine/hollow carbon spheres coupled with dendritic Au@Rh nanocrystals. Microchim Acta 189, 271 (2022). https://doi.org/10.1007/s00604-022-05372-9

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