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Easy and versatile cellulosic support inhibiting broad spectrum strains: synergy between photodynamic antimicrobial therapy and polymyxin B

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Abstract

Despite advances achieved in the health field over the last decade, infections caused by resistant bacterial strains are an increasingly important societal issue that needs to be addressed. New approaches have already been developed to overcome this problem. Photodynamic antimicrobial chemotherapy (PACT) could provide a promising alternative method to eradicate microbes. This approach has already inspired the development of innovative surfaces. Interesting results were achieved against Gram-positive bacteria, but it also appeared that Gram-negative strains, especially Pseudomonas aeruginosa, were less sensitive to PACT. However, materials coated with cationic porphyrins have already proven their wide-spectrum activity, but these materials were not suitable for industrial-scale production. The main aim of this work was the design of a large-scale evolutionary material based on PACT and antibiotic prophylaxis. Transparent regenerated cellulose has been simply impregnated with a usual cationic porphyrin (N-methylpyridyl) and an antimicrobial peptide (polymyxin B). In addition to its photophysical properties, this film exhibited a wide-spectrum bactericidal activity over 4 days despite daily application of fresh bacterial inoculums. The efficiency of PACT and polymyxin B combination could help to reduce the emergence of bacterial multi-resistant strains and we believe that this kind of material would provide an excellent opportunity to prevent bacterial contamination of bandages or packaging.

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The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors thank the “Conseil Regional du Limousin” for financial support (FLG) and are indebted to Dr. Michel Guilloton for manuscript editing.

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Authors and Affiliations

  1. Univ Limoges, LABCiS, UR22722, 87000, Limoges, France

    Florent Le Guern, Tan-Sothea Ouk & Vincent Sol

  2. Institut Lavoisier de Versailles, Université Paris-Saclay, UVSQ, CNRS, 78000, Versailles, France

    Florent Le Guern

  3. Université de Lorraine, CNRS LRGP UMR 7274, 54000, Nancy, France

    Phillipe Arnoux & Céline Frochot

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  1. Florent Le Guern
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Le Guern, F., Ouk, TS., Arnoux, P. et al. Easy and versatile cellulosic support inhibiting broad spectrum strains: synergy between photodynamic antimicrobial therapy and polymyxin B. Photochem Photobiol Sci 23, 395–407 (2024). https://doi.org/10.1007/s43630-023-00526-x

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