Abstract
Male infertility contributes as the main factor in 30–50% of infertility cases. Conventional methods for sperm preparation have induced questioning of sperm recovery rates. The microfluidic sperm sorting (MSS) technique selects highly motile sperm with lower levels of SDF (sperm DNA fragmentation) compared to conventional sperm sorting techniques. This study aimed to determine whether utilizing this technique will reveal better embryo quality and euploidy rates in couples with repeated implantation failure (RIF) and high SDF in a new PGT-A (preimplantation genetic testing for aneuploidies) cycle. This retrospective study included couples referred to PGT-A for previous repeated ART (assisted reproductive techniques) cycle failures and with high SDF. In their new cycles, couples who accepted the technique were assigned to the MSS group, and the rest were managed with DGC (density-gradient centrifugation). Two groups were compared in terms of fertilization and euploidy rates, clinical miscarriage and live birth rates, the total number of blastocysts, and top quality blastocysts. There was no difference between the groups regarding fertilization rates, euploidy rates, clinical miscarriage, and live birth rates. The total number of blastocysts and top quality blastocysts were significantly higher in the MSS group. The MSS technique provides a higher number of top-quality blastocysts than DGC; however, neither euploidy nor live birth rates improved. Studies focusing on confounding factors to embryonic genomic status in the presence of high SDF are needed.
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The data that support the study’s findings are available from the corresponding author upon reasonable request.
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by E.G. Pabuçcu, M. Keskin, and T. Arslanca. The first draft of the manuscript was written by M. Keskin and E.G. Pabuçcu, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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This is an observational retrospective study. The Ufuk University Research Ethics Committee has confirmed that no ethical approval is required.
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Keskin, M., Pabuçcu, E.G., Arslanca, T. et al. Does Microfluidic Sperm Sorting Affect Embryo Euploidy Rates in Couples with High Sperm DNA Fragmentation?. Reprod. Sci. 29, 1801–1808 (2022). https://doi.org/10.1007/s43032-021-00784-z
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DOI: https://doi.org/10.1007/s43032-021-00784-z