Abstract
Soil microorganisms play a crucial role in nutrient cycling, maintaining soil fertility, and carbon sequestration in soil. However, our understanding of the adaptive mechanisms and driving factors of microbial community structure in non-calcareous fluvo-aquic soil under different fertilization treatments remains limited. A field experiment was designed with winter wheat-summer maize rotation system started in 1978. Five treatments were selected: CK (no fertilizers); N (nitrogen); NPK (nitrogen, phosphorus and potassium); M (60,000 kg pig manure ha−1); MN (60,000 kg pig manure + 276 kg N ha−1). The results showed that N, NPK, M and MN treatment significantly increased soil organic carbon (SOC) and total nitrogen (TN) by 22.7% and 38.8%, 23.1% and 30.5%, 113.4% and 141.6%, 167.1% and 304.1% compared to CK, respectively. Long-term fertilization (NPK, M, and MN) alters the structure of soil microbial communities through improvements in soil carbon and nitrogen cycles and soil enzyme activities. Additionally, pig manure application significantly enhanced the relative abundance of Pezizomycetes (35.9%) and Firmicutes (23.4%). Redundancy analysis (RDA) indicated that TN and SOC are the primary factors driving the changes in soil bacterial communities. TN and catalase activity are the main factors driving the changes in the soil fungal community. Overall, long-term fertilization, especially the addition of organic resources, can strengthen the biological regulation processes in soil ecosystems to maintain or enhance soil fertility.
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References
AdetunjiI AT, Lewu FB, Mulidzi R, Ncube B (2017) The biological activities of β-glucosidase, phosphatase and urease as soil quality indicators: A review. J Soil Sci Plant Nut 17:794–807. https://doi.org/10.4067/S0718-95162017000300018
Ai C, Zhang S, Zhang X, Guo D, Zhou W, Huang S (2018) Distinct responses of soil bacterial and fungal communities to changes in fertilization regime and crop rotation. Geoderma 319:156–166. https://doi.org/10.1016/j.geoderma.2018.01.010
Bai R, Wang JT, Deng Y, He JZ, Feng K, Zhang LM (2017) Microbial community and functional structure significantly varied among distinct types of paddy soils but responded differently along gradients of soil depth layers. Front Microbiol 8:945. https://doi.org/10.3389/fmicb.2017.00945
Bao SD (2000) Soil Agrochemical Analysis, 3rd edn. China Agriculture Press, Beijing, China
Burns RG, DeForest JL, Marxsen J, Sinsabaugh RL, Stromberger ME, Wallenstein MD, Weintraub MN, Zoppini A (2013) Soil enzymes in a changing environment: current knowledge and future directions. Soil Biol Biochem 58:216–234. https://doi.org/10.1016/j.soilbio.2012.11.009
Chen Y, Cao Y, Liu S (2021) Effects of Long-Term Mineral Fertilizer Application on Soil Nutrients, Yield, and Fungal Community Composition. Eurasian Soil Sc 54:597–604. https://doi.org/10.1134/S1064229321040049
Chuan X, Carlyle CN, Bork EW, Chang SX, Hewins DB (2020) Extracellular enzyme activity in grass litter varies with grazing history, environment and plant species in temperate grasslands. Sci Total Environ 702:134562. https://doi.org/10.1016/j.scitotenv.2019.134562
Čuhel J, Malý S, Královec J (2019) Shifts and recovery of soil microbial communities in a 40-year field trial under mineral fertilization. Pedobiologia 77:150575. https://doi.org/10.1016/j.pedobi.2019.150575
Dan Y, Wang X, Ji M, Sang W, Shen Z, Zhang Y (2023) Influence of temperature change on the immobilization of soil Pb and Zn by hydrochar: Roles of soil microbial modulation. Environ Pollut 320:121109. https://doi.org/10.1016/j.envpol.2023.121109
De Mastro F, Brunetti G, Traversa A, Blagodatskaya E (2022) Fertilization promotes microbial growth and minimum tillage increases nutrient-acquiring enzyme activities in a semiarid agro-ecosystem. Appl Soil Ecol 177:104529. https://doi.org/10.1016/j.apsoil.2022.104529
Dinesh R, Srinivasan V, Hamza S, Manjusha A (2010) Short-term incorporation of organic manures and biofertilizers influences biochemical and microbial characteristics of soils under an annual crop [Turmeric (Curcuma longa L.)]. Bioresour Technol 101:4697–4702. https://doi.org/10.1016/j.biortech.2010.01.108
FAO (2021) FAO Stat 2021. Rome, Italy: Food and Agriculture Organization of the United Nations. https://www.fao.org/3/cb4477en/cb4477en.pdf
Fierer N, Breitbart M, Nulton J, Salamon P, Lozupone C, Jones R, Robeson M, Edwards RA, Felts B, Rayhawk S, Knight R, Rohwer F, Jackson RB (2007) Metagenomic and small-subunit rRNA analyses reveal the genetic diversity of bacteria, archaea, fungi, and viruses in soil. Appl Environ Microbiol 73:7059–7066. https://doi.org/10.1128/aem.00358-07
Fierer N (2017) Embracing the unknown: disentangling the complexities of the soil microbiome. Nat Rev Microbiol 15:579–590. https://doi.org/10.1038/nrmicro.2017.87
Geisseler D, Linquist BA, Lazicki PA (2017) Effect of fertilization on soil microorganisms in paddy rice systems - A meta-analysis. Soil Biol Biochem 115:452–460. https://doi.org/10.1016/j.soilbio.2017.09.018
Grosso F, Bååth E, De Nicola F (2016) Bacterial and fungal growth on different plant litter in Mediterranean soils: Effects of C/N ratio and soil pH. Appl Soil Ecol 108:1–7. https://doi.org/10.1016/j.apsoil.2016.07.020
Han J, Dong Y, Zhang M (2021) Chemical fertilizer reduction with organic fertilizer effectively improve soil fertility and microbial community from newly cultivated land in the Loess Plateau of China. Appl Soil Ecol 165:103966. https://doi.org/10.1016/j.apsoil.2021.103966
Hansen K, Perry BA, Dranginis AW, Pfister DH (2013) A phylogeny of the highly diverse cup-fungus family Pyronemataceae (Pezizomycetes, Ascomycota) clarifies relationships and evolution of selected life history traits. Mol Phylogenet Evol 67:311–335. https://doi.org/10.1016/j.ympev.2013.01.014
Huang R, McGrath SP, Hirsch PR, Clark IM, Storkey J, Wu L, Zhou J, Liang Y (2019) Plant-microbe networks in soil are weakened by century-long use of inorganic fertilizers. Microb Biotechnol 12:1464–1475. https://doi.org/10.1111/1751-7915.13487
Inubushi K, Acquaye S (2004) Role of microbial biomass in biogeochemical processes in paddy soil environments. Soil Sci Plant Nutr 50:793–805. https://doi.org/10.1080/00380768.2004.10408539
Jangid K, Williams MA, Franzluebbers AJ, Sanderlin JS, Reeves JH, Jenkins MB, Endale DM, Coleman DC, Whitman WB (2008) Relative impacts of land-use, management intensity and fertilization upon soil microbial community structure in agricultural systems. Soil Biol Biochem 40:2843–2853. https://doi.org/10.1016/j.soilbio.2008.07.030
Jin H, Zhang D, Yan Y, Yang C, Fang B, Li X, Shao Y, Wang H, Yue J, Wang Y, Cheng H, Shi Y, Qin F (2022) Short-term application of chicken manure under different nitrogen rates alters structure and co-occurrence pattern but not diversity of soil microbial community in wheat field. Front Microbiol 13:975571. https://doi.org/10.3389/fmicb.2022.975571
Joergensen RG, Brookes PC (2005) Quantification of soil microbial bio-mass by fumigation-extraction. In: Monit Assess Soil Bioremediat 281–295. https://doi.org/10.1007/3-540-28904-6_14
Krajewska B (2009) Ureases I. Functional, catalytic and kinetic properties: A review. J Mol Catal B-Enzym 59:9–21. https://doi.org/10.1016/j.molcatb.2009.01.003
Lan Z, Zhao Y, Zhang J, Yang X, Sial TA, Khan MN (2020) Effects of the long-term fertilization on pore and physicochemical characteristics of loess soil in Northwest China. Agron J 112:4741–4751. https://doi.org/10.1002/agj2.20401
Lazcano C, Gómez-Brandón M, Revilla P, Domínguez J (2012) Short-term effects of organic and inorganic fertilizers on soil microbial community structure and function. Biol Fert Soils 49:723–733. https://doi.org/10.1007/s00374-012-0761-7
Li J, Cooper JM, Lin Z, Li Y, Yang X, Zhao B (2015) Soil microbial community structure and function are significantly affected by long-term organic and mineral fertilization regimes in the North China Plain. Appl Soil Ecol 96:75–87. https://doi.org/10.1016/j.apsoil.2015.07.001
Li W, Zhang Y, Mao W, Wang C, Yin S (2020) Functional potential differences between Firmicutes and Proteobacteria in response to manure amendment in a reclaimed soil. Can J Microbiol 66:689–697. https://doi.org/10.1139/cjm-2020-0143
Liu S, Wang J, Pu S, Blagodatskaya E, Kuzyakov Y, Razavi BS (2020) Impact of manure on soil biochemical properties: A global synthesis. Sci Total Environ 745:141003. https://doi.org/10.1016/j.scitotenv.2020.141003
Luo P, Han X, Wang Y, Han M, Shi H, Liu N, Bai H (2015) Influence of long-term fertilization on soil microbial biomass, dehydrogenase activity, and bacterial and fungal community structure in a brown soil of northeast China. Ann Microbiol 65:533–542. https://doi.org/10.1007/s13213-014-0889-9
Maharachchikumbura SS, Guo LD, Chukeatirote E, McKenzie EH, Hyde KD (2013) A destructive new disease of Syzygium samarangense in Thailand caused by the new species Pestalotiopsis samarangensis. Trop Plant Pathol 38:227–235. https://doi.org/10.1590/s1982-56762013005000002
Meidute S, Demoling F, & Bååth E (2008) Antagonistic and synergistic effects of fungal and bacterial growth in soil after adding different carbon and nitrogen sources. Soil Biol Biochem 40:2334–2343. https://doi.org/10.1016/j.soilbio.2008.05.011
Naether A, Foesel BU, Naegele V, Wust PK, Weinert J, Bonkowski M, Alt F, Oelmann Y, Polle A, Lohaus G, Gockel S, Hemp A, Kalko EK, Linsenmair KE, Pfeiffer S, Renner S, Schoning I, Friedric MW (2012) Environmental factors affect Acidobacterial communities below the subgroup level in grassland and forest soils. Appl Environ Microbiol 78:7398–7406. https://doi.org/10.1128/AEM.01325-12
Ning Q, Chen L, Jia Z, Zhang C, Ma D, Li F, Zhang J, Li D, Han X, Cai Z, Huang S, Liu W, Zhu B, Li Y (2020) Multiple long-term observations reveal a strategy for soil pH-dependent fertilization and fungal communities in support of agricultural production. Agr Ecosyst Environ 293:106837. https://doi.org/10.1016/j.agee.2020.106837
Pu XZ, Zhang GJ, Zhang PP, Liu YJ, Zhang WF (2016) Effects of straw management, inorganic fertiliser, and manure amendment on soil microbial properties, nutrient availability, and root growth in a drip-irrigated cotton field. Crop Pasture Sci 67:1297–1308. https://doi.org/10.1071/CP16230
Sang S, Dai H, Hu BX, Huang Z, Liu Y, Xu L (2022) Distribution characteristics and factors influencing microbial communities in the core soils of a seawater intrusion area in Longkou City, China. Hydrogeol J 30:1833–1845. https://doi.org/10.1007/s10040-022-02511-7
Schlüter S, Großmann C, Diel J, Wu GM, Tischer S, Deubel A, Rücknagel J (2018) Long-term effects of conventional and reduced tillage on soil structure, soil ecological and soil hydraulic properties. Geoderma 332:10–19. https://doi.org/10.1016/j.geoderma.2018.07.001
Shen H, Wang B, Jiao Y, Zhang X, Zhang Q, Xiong Z (2023) Bacteria are more sensitive than fungi to soil fertility in an intensive vegetable field. Appl Soil Ecol 190:105003. https://doi.org/10.1016/j.apsoil.2023.105003
Shu X, He J, Zhou Z, Xia L, Hu Y, Zhang Y, Zhang Y, Luo Y, Chu H, Liu W, Yuan S, Gao X, Wang C (2022) Organic amendments enhance soil microbial diversity, microbial functionality and crop yields: A meta-analysis. Sci Total Environ 829:154627. https://doi.org/10.1016/j.scitotenv.2022.154627
Siles JA, Margesin R (2016) Abundance and diversity of bacterial, archaeal, and fungal communities along an altitudinal gradient in alpine forest soils: what are the driving factors? Microb Ecol 72:207–220. https://doi.org/10.1007/s00248-016-0748-2
Six J, Frey SD, Thiet RK, Batten KM (2006) Bacterial and fungal contributions to carbon sequestration in agroecosystems. Soil Sci Soc Am J 70:555–569. https://doi.org/10.2136/sssaj2004.0347
Sun R, Guo X, Wang D, Chu H (2015a) Effects of long-term application of chemical and organic fertilizers on the abundance of microbial communities involved in the nitrogen cycle. Appl Soil Ecol 95:171–178. https://doi.org/10.3390/su142315658
Sun R, Zhang XX, Guo X, Wang D, Chu H (2015b) Bacterial diversity in soils subjected to long-term chemical fertilization can be more stably maintained with the addition of livestock manure than wheat straw. Soil Biol Biochem 88:9–18. https://doi.org/10.1016/j.soilbio.2015.05.007
Tedersoo L, Arnold AE, Hansen K (2013) Novel aspects in the life cycle and biotrophic interactions in Pezizomycetes (Ascomycota, Fungi). Mol Ecol 22:1488–1493. https://doi.org/10.1111/mec.12224
Wakelin SA, Colloff MJ, Harvey PR, Marschner P, Gregg AL, Rogers SL (2007) The effects of stubble retention and nitrogen application on soil microbial community structure and functional gene abundance under irrigated maize. FEMS Microbiol Ecol 59:661–670. https://doi.org/10.1111/j.1574-6941.2006.00235.x
Wang Z, Liu Y, Zhao L, Zhang W, Liu L (2019) Change of soil microbial community under long-term fertilization in a reclaimed sandy agricultural ecosystem. Peer J 7:6497. https://doi.org/10.7717/peerj.6497
Wang H, Ren T, Muller K, Van Zwieten L, Wang H, Feng H, Xu C, Yun F, Ji X, Yin Q, Shi H, Liu G (2021) Soil type regulates carbon and nitrogen stoichiometry and mineralization following biochar or nitrogen addition. Sci Total Environ 753:141645. https://doi.org/10.1111/gcb.16361
Wang J, Xie J, Li L, Effah Z, Xie L, Luo Z, Zhou Y, Jiang Y (2022) Fertilization treatments affect soil CO2 emission through regulating soil bacterial community composition in the semiarid Loess Plateau. Sci Rep 12:20123. https://doi.org/10.1038/s41598-022-21108-4
Wei M, Hu G, Wang H, Bai E, Lou Y, Zhang A, Zhuge Y (2017) 35 years of manure and chemical fertilizer application alters soil microbial community composition in a Fluvo-aquic soil in Northern China. Eur J Soil Biol 82:27–34. https://doi.org/10.1016/j.ejsobi.2017.08.002
Wei L, Ge T, Zhu Z, Ye R, Peñuelas J, Li Y, Lynn TM, Jones DL, Wu J, Kuzyakov Y (2022) Paddy soils have a much higher microbial biomass content than upland soils: A review of the origin, mechanisms, and drivers. Agr Ecosyst Environ 326:107798. https://doi.org/10.1016/j.agee.2021.107798
Wu CX, Yan BS, Wei FR, Wang HL, Gao LQ, Ma HZ, Liu Q, Liu Y, Liu GB, Wang GQ (2023) Long-term application of nitrogen and phosphorus fertilizers changes the process of community construction by affecting keystone species of crop rhizosphere microorganisms. Sci Total Environ 897:165239. https://doi.org/10.1016/j.scitotenv.2023.165239
Xin XL, Zhang JB, Zhu AN, Zhang CZ (2016) Effects of long-term (23 years) mineral fertilizer and compost application on physical properties of fluvo-aquic soil in the North China Plain. Soil Till Res 156:166–172.https://doi.org/10.1016/j.still.2015.10.012
Yang Y, Ji R, Zhang H, Christie P, Feng G, Li X, Gai J (2021) Stoichiometric analysis of an arable crop–soil–microbe system after repeated fertilizer and compost application for 10 years. J Soils Sediments 21:1466–1475. https://doi.org/10.1007/s11368-021-02896-0
Yang Y, Chen X, Liu L, Li T, Dou Y, Qiao J, Wang Y, An S, Chang SX (2022) Nitrogen fertilization weakens the linkage between soil carbon and microbial diversity: A global meta-analysis. Glob Chang Biol 28:6446–6461. https://doi.org/10.1016/j.scitotenv.2020.141645
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This research was financially supported by the Natural Science Foundation of Shandong Province (ZR2021QC107), the Modern Agricultural Technology and Industry System of Shandong Province (SDAIT-02-06), the Central Government to Guide Local Special Plans for Science and Technology Development (22-1-3-6-zyyd-nsh).
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Xu, X., Wang, J., Niu, Y. et al. 44-Years of Fertilization Altered Soil Microbial Community Structure by Changing Soil Physical, Chemical Properties and Enzyme Activity. J Soil Sci Plant Nutr (2024). https://doi.org/10.1007/s42729-024-01740-x
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DOI: https://doi.org/10.1007/s42729-024-01740-x