Abstract
It is urgent to find ways to utilise the accumulated recalcitrant phosphorus (P) in soil, as natural rock phosphate reserves are at a verge of depletion. Phosphate-solubilising microorganisms (PSMs) can be a cost-effective and eco-friendly approach to address the problem. A pot culture experiment was carried out to evaluate the changes in various inorganic fractions of P in an Inceptisol vis a vis their availability to the plant using PSMs. A bulk surface soil sample was obtained from New Delhi (pH = 8.30) and after processing, three levels of P and three levels of PSM (No-PSM, phosphate-solubilising bacteria (PSB), and phosphate-solubilising fungi (PSF)) were applied in a completely randomised design with three replications. Soybean (Glycine max) was grown as test crop. By and large, various P fractions followed the order: Ca-bound P (Ca-P, 46.9%) > residual-P (RES-P, 22.8%) > reductant-soluble P (RS-P, 11.6%) > Al-bound P (Al-P, 11.0%) > Fe-bound P (Fe–P, 4.06%) > occluded P (OC-P, 2.51%) > soluble and loosely bound-P (SL-P, 1.21%). Application of PSB significantly increased the SL-P by solubilising the Al-P, Fe–P and Ca-P in soil. The PSB increased the dry-matter yield of soybean by ~ 8.41%, whereas, applied PSF had no effect on either soil P fractions or plant dry-matter yield. Combined application of a reduced dose of P along with PSB can be practised in order to utilise native soil P without compromising the crop yield.
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References
Acosta-Martinez V, Tabatabai MA (2000) Enzyme activities in a limed agricultural soil. Biol Fertil Soils 31:85–91. https://doi.org/10.1007/s003740050628
Alam K, Barman M, Datta SP, Kumar S, Annapurna K, Shukla L, Chakraborty D (2020) Efficacy of phosphate solubilizing microorganisms in utilizing native phosphorus in an alkaline alluvial soil of North India. Indian J Agric Sci 90:2199–2203
Alam K, Barman M, Datta SP, Annapurna K, Shukla L, Ray P (2022) Application of phosphate solubilizing fungi and lime altered the soil inorganic phosphorus fractions in an Ultisol of north-eastern India. Soil Sci Plant Nut 68:409–420. https://doi.org/10.1080/00380768.2022.2094204
Alori ET, Glick BR, Babalola OO (2017) Microbial phosphorus solubilization and its potential for use in sustainable agriculture. Front Microbiol 8:971. https://doi.org/10.3389/fmicb.2017.00971
Bajpai PD, Sundara R, WVB. (1971) Phosphate solubilising bacteria. Soil Sci Plant Nut 17:46–53
Balemi T, Negisho K (2012) Management of soil phosphorus and plant adaptation mechanisms to phosphorus stress for sustainable crop production: a review". J Soil Sci Plant Nutr 12:547–562. https://doi.org/10.4067/S0718-95162012005000015
Bargaz A, Lyamlouli K, Chtouki M, Zeroual Y, Dhiba D (2018) Soil microbial resources for improving fertilizers efficiency in an integrated plant nutrient management system. Front Microbiol 9:1606. https://doi.org/10.3389/2Ffmicb.2018.01606
Barman M, Shukla LM, Datta SP, Rattan RK (2014) Effect of applied lime and boron on the availability of nutrients in acid soil. J Plant Nutr 37:357–373. https://doi.org/10.1080/01904167.2013.859698
Barman M, Datta SP, Rattan RK, Meena MC (2015) Chemical fractions and bioavailability of nickel in alluvial soils. Plant Soil Environ 61:17–22. https://doi.org/10.17221/613/2014-PSE
Behera BC, Yadav H, Singh SK, Sethi BK, Mishra RR, Kumari S, Thatoi H (2017) Alkaline phosphatase activity of a phosphate solubilizing Alcaligenes faecalis, isolated from Mangrove soil. Biotech Res Innov 1:101–111
Bhattacharyya T, Pal DK, Mandal C, Chandran P, Ray SK, Sarkar D, Velmourougane K, Srivastava A, Sidhu GS, Singh RS, Sahoo AK (2013) Soils of India: historical perspective, classification and recent advances. Curr Sci 104:1308–1323
Boschetti NG, Quintero CE, Giuffre L (2009) Phosphorus fractions of soils under Lotus corniculatus as affected by different phosphorus fertilizers. Biol Fertil Soils 45:379–384. https://doi.org/10.1007/s00374-008-0341-z
Chang SC, Jackson ML (1957) Fractionation of soil phosphorus. Soil Sci 84:133–144
Clarholm M (1993) Microbial biomass P, labile P, and acid phosphatase activity in the humus layer of a spruce forest, after repeated additions of fertilizers. Biol Fertil Soils 16:287–292. https://doi.org/10.1007/BF00369306
Datta SP, Rattan RK, Suribabu K, Datta SC (2002) Fractionation and colorimetric determination of boron in soils. J Plant Nutr Soil Sci 165:179–184. https://doi.org/10.1002/1522-624(200204)165:2%3C179::AIDJPLN179%3E3.0.CO;2-Q
Deubel A, Gransee A, Merbach W (2000) Transformation of organic rhizodepositions by rhizosphere bacteria and its influence on the availability of tertiary calcium phosphate. J Plant Nutr Soil Sci 163:387–392. https://doi.org/10.1002/1522-2624(200008)163:4%3c387::AID-JPLN387%3e3.0.CO;2-K
Etesami H, Jeong BR, Glick BR (2021) Contribution of arbuscular mycorrhizal fungi, phosphate-solubilizing bacteria, and silicon to P uptake by plant: a review. Front Plant Sci 12:1355. https://doi.org/10.3389/fpls.2021.699618
Gaur AC (1990) Phosphate solubilizing microorganisms as biofertilizers. Omega scientific publishers, New Delhi
Goldstein AH (2000) Bioprocessing of rock phosphate ore: essential technical considerations for the development of a successful commercial technology. In: Proceedings of the 4th International Fertilizer Association Technical Conference, IFA, Paris, vol. 220
Hesse PR (2002) Cation and anion exchange properties. A textbook of soil chemical analysis. CBS Publishers and distributors, New Delhi, pp 88–105
Illmer P, Barbato A, Schinner F (1995) Solubilization of hardlysoluble AlPO4 with P-solubilizing microorganisms. Soil Bio Biochem 27:265–270
Jackson ML (1973) Soil Chemical Analysis. Prentice Hall of India Pvt. Ltd., New Delhi
Jin JY, Martens DC, Zelazny LW (1987) Distribution and plant availability of soil boron fractions. Soil Sci Soc Am J 51:1228–1231. https://doi.org/10.2136/sssaj1987.03615995005100050025x
Kafle A, Cope KR, Raths R, Krishna Yakha J, Subramanian S, Bücking H, Garcia K (2019) Harnessing soil microbes to improve plant phosphate efficiency in cropping systems. Agron 9:127. https://doi.org/10.3390/agronomy9030127
Kalayu G (2019) Phosphate solubilizing microorganisms: promising approach as biofertilizers. Int J Agron 1–7. https://doi.org/10.1155/2019/4917256
Khan MS, Zaidi A, Wani PA (2007) Role of phosphate-solubilizing microorganisms in sustainable agriculture—a review. Agron Sustain Dev 27:29–43
Khan AA, Jilani G, Akhtar MS, Naqvi SMS, Rasheed M (2009) Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J Agric Biol Sci 1:48–58
Kuo S (1996) Phosphorus. In: Sparks DL (ed) Methods of Soil Analysis Part 3: Chemical Methods, SSSA Book Series 5. Soil Science Society of America, Madison, Wisconsin, pp 869–920
Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Sci Soc Am J 42:421–428. https://doi.org/10.2136/sssaj1978.03615995004200030009x
MacDonald K (1961) The hydrolysis of phenyl phosphate by mouse-liver acid phosphatase. Biochem 80:154–161. https://doi.org/10.1042/bj0800154
Manojlovic D, Todorovic M, Jovicic J, Krsmanovic VD, Pfendt PA, Golubovic R (2007) Preservation of water quality in accumulation Lake Rovni: the estimate of the emission of phosphorus from inundation area. Desalination 213:104–109. https://doi.org/10.1016/j.desal.2006.05.058
Marwaha BC (1995) Biofertilizer- a supplementary source of plant nutrient. Fert News (India) 40:39–50
Menezes-Blackburn D, Giles C, Darch T, George TS, Blackwell M, Stutter M, Shand C, Lumsdon D, Cooper P, Wendler R, Brown L (2018) Opportunities for mobilizing recalcitrant phosphorus from agricultural soils: a review. Plant Soil 427:5–16. https://doi.org/10.1007/s11104-017-3362-2
Owen D, Williams AP, Withers GGW, PJA, (2015) Use of commercial bio-inoculants to increase agricultural production through improved phosphrous acquisition. Applied Soil Ecol 86:41–54
Page AL, Miller RH, Keeney DR (1982) Methods of soil analysis. American Society of Agronomy, Madison
Pattanayak SK, Sureshkumar P, Tarafdar JC (2009) New vista in phosphorus research. J Indian Soc Soil Sci 57:536–545
Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 17:362–370
Richards LA (1954) Diagnosis and Improvement of Saline Alkali Soils, Agriculture, 160, Handbook 60. US Department of Agriculture, Washington DC
Roberts TL, Johnston AE (2015) Phosphorus use efficiency and management in agriculture. Resour Conserv Recycl 105:275–281. https://doi.org/10.1016/j.resconrec.2015.09.013
Samadi A (2006) Contribution of inorganic phosphorus fractions to plant nutrition in alkaline-calcareous soils. J Agric Sci Technol 8:77–89
Sanyal SK, Dwivedi BS, Singh VK, Majumdar K, Datta SC, Pattanayak SK, Annapurna K (2015) Phosphorus in relation to dominant cropping sequences in India: chemistry, fertility relations and management options. Curr Sci 108:1262–1270
Sattari SZ, Bouwman AF, Giller KE, van Ittersum MK (2012) Residual soil phosphorus as the missing piece in the global phosphorus crisis puzzle. Proc Natl Acad Sci 109:6348–6353. https://doi.org/10.1073/pnas.1113675109
Sharma A, Rawat US, Yadav BK (2012) Influence of phosphorus levels and phosphorus solubilizing fungi on yield and nutrient uptake by wheat under sub-humid region of Rajasthan, India. Int Sch Res Notices 2012: 1–9. https://doi.org/10.5402/2012/234656
Sharma PK, Parmar DK (1998) The effect of phosphorus and mulching on the efficiency of phosphorus use and productivity of wheat grown on a mountain Alfisol in the Western Himalayas. Soil Use Manag 14:25–29. https://doi.org/10.1111/j.1475-2743.1998.tb00606.x
Singh H, Reddy MS (2011) Effect of inoculation with phosphate solubilizing fungus on growth and nutrient uptake of wheat and maize plants fertilized with rock phosphate in alkaline soils. Eur J Soil Biol 47:30–34. https://doi.org/10.1016/j.ejsobi.2010.10.005
Snedecor GW, Cochran WG (1967) Statistical methods, 6th edn. Iowa State University Press, Iowa, USA
Staunton S, Leprince F (1996) Effect of pH and some organic anions on the solubility of soil phosphate: implications for P bioavailability. Eur J Soil Sci 47:231–239. https://doi.org/10.1111/j.1365-2389.1996.tb01394.x
Subbiah BV, Asija GL (1956) A rapid procedure for estimation of available nitrogen in soil. Curr Sci 25:259–260
Tabatabai MA, Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307. https://doi.org/10.1016/0038-0717(69)90012-1
Tariq MR, Shaheen F, Mustafa S, Sajid AL, Fatima A, Shafiq M, Safdar W, Sheas MN, Hameed A, Nasir MA (2022) Phosphate solubilizing microorganisms isolated from medicinal plants improve growth of mint. Peer J 10:13782. https://doi.org/10.7717/2Fpeerj.13782
Tian J, Ge F, Zhang D, Deng S, Liu X (2021) Roles of phosphate solubilizing microorganisms from managing soil phosphorus deficiency to mediating biogeochemical P cycle. Biol 10:158. https://doi.org/10.3390/2Fbiology10020158
Walkley A, Black IA (1934) An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38
Wani P, Khan M, Zaidi A (2007) Co-inoculation of nitrogen-fixing and phosphate-solubilizing bacteria to promote growth, yield and nutrient uptake in chickpea. Acta Agron Hung 55:315–323. https://doi.org/10.1556/AAgr.55.2007.3.7
Watanabe FS, Olsen SR (1965) Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from soil. Soil Sci Soc Am J 29:677–678. https://doi.org/10.2136/sssaj1965.03615995002900060025x
Whitelaw MA (1999) Growth promotion of plants inoculated with phosphate-solubilizing fungi. Adv Agron 69:99–151. https://doi.org/10.1016/S0065-2113(08)60948-7. (Academic Press)
Zhang TQ, MacKenzie AF (1997) Changes of soil phosphorous fractions under long-term corn monoculture. Soil Sci Soc Am J 61:485–493. https://doi.org/10.2136/sssaj1997.03615995006100020017x
Acknowledgements
The authors thank Director, ICAR-IARI for his constant support during the study. The financial support received by the first author during the M.Sc. course in the form of Junior Research Fellowship (JRF) from the Indian Council of Agricultural Research (ICAR) is hereby acknowledged.
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Indian Agricultural Research Institute (ICAR-IARI), New Delhi, India.
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Alam, K., Barman, M., Datta, S.P. et al. Modification of Inorganic Fractions of Phosphorus by Phosphate-Solubilising Microorganisms in Conjunction with Phosphorus Fertilisation in a Tropical Inceptisol. J Soil Sci Plant Nutr 23, 2488–2497 (2023). https://doi.org/10.1007/s42729-023-01206-6
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DOI: https://doi.org/10.1007/s42729-023-01206-6