Abstract
The study was conducted to evaluate the efficacy of salicylic acid foliar spray against Chilli Veinal Mottle Virus (ChiVMV) infecting Capsicum annuum L. which induces systemic acquired resistance. Different concentrations (50, 100, and 150 ppm) of salicylic acid were sprayed on pepper seedlings and were assessed for the levels of defense-related enzymes (POD, PPO, SOD, and catalase), photosynthetic pigments, total phenols, total proteins, and total carbohydrates, MDA for lipid peroxidation, and gene expression analysis of three defense-related marker genes. 100 ppm salicylic acid enhanced the defensive enzymes, phenols, and MDA levels and helped in mitigating their level gradually. It also aided in restoring the chlorophyll, carotenoids, total carbohydrates, and total protein level in the inoculated plants when compared to untreated inoculated and healthy plants. The mechanism associated with the plant resistance was confirmed by evaluating PAL, NPR1, and JAZ3 gene expression, which were up-regulated depicting the salicylic acid-induced systemic acquired resistance and jasmonic acid-mediated pathways. Hence, proving that salicylic acid is an effective resistance inducer in pepper plants against ChiVMV.
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Acknowledgments
The authors are grateful to the Director, ICAR-Indian Institute of Horticultural Research, Bengaluru for providing the research facilities and support. The research was financially supported by the project on the “Consortium platform on Vaccines and diagnostics”, the Indian Council of Agricultural Research, Government of India, New Delhi, India.
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SS designed the work, performed the laboratory work, validated the results, and wrote the manuscript. GAG contributed to the interpretation of the results. KSS supervised the biochemistry work and provided the lab facilities. MKR provided the resources, supervised the work, revised the paper, and approved the final version of the article.
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Subhash, S., Geetha, G.A., Shivashankar, K.S. et al. Pepper-acquired resistance induced by salicylic acid against Chilli Veinal Mottle Virus. Indian Phytopathology 75, 1159–1166 (2022). https://doi.org/10.1007/s42360-022-00561-1
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DOI: https://doi.org/10.1007/s42360-022-00561-1