Abstract
Ethyl acetate crude extract obtained from fermented cultures of Phomopsis sp. strain S4 (strain S4 extract) was effective in the control of mycelial growth and influenced to some extent sclerotia germination and the number of sclerotia formed by Sclerotinia sclerotiorum, the major agent of Sclerotinia disease. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) studies showed that in the presence of strain S4 extract, the general hyphal morphology and cells ultrastructure of S. sclerotiorum exhibited drastic changes, including hyphae shriveled and full of holes, damaged cell wall, incomplete plasma membrane, empty cells deprived of cytoplasm, and organelles. Leakage of carbohydrates in S. sclerotiorum exposed to strain S4 extract was also detected. Moreover, the methane dicarboxylic aldehyde (MDA) content increased whereas the catalase (CAT) content was lower. In addition, strain S4 extract was analyzed by gas chromatography mass spectrometry (GC-MS). Of the 86 compounds identified in the strain S4 extract the most frequent were those with a benzene ring (60.91%). These findings suggest that strain S4 extract has potential as natural antifungal agent against S. sclerotiorum and that may be due to its ability to cause oxidative damage to the pathogen cells.
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References
Abawi GS, Grogan RG (1979) Epidemiology of diseases caused by Sclerotinia species. Phytopathology 69:899–904
Al-Reza SM, Rahman A, Ahmed Y, Kang SC (2010) Inhibition of plant pathogens in vitro and in vivo with essential oil and organic extract of Cestrum nocturnum L. Pestic Biochem Physiol 96:86–92
Barnard C, Padgitt M, Uri ND (1997) Pesticides use and its measurement. Int Pest Control 39:161–164
Boland GJ, Hall R (1994) Index of plant hosts of Sclerotinia sclerotiorum. Can J Plant Pathol 16:93–108
Burke BA, Nair MG (1989) Antimicrobial/antifungal compositions. United States Utility Patent, US4876277
Corrado M, Rodrigues KF (2004) Antimicrobial evaluation of fungal extract produced by endophytic strains of Phomopsis sp. J Basic Microbiol 44:157–160
Du ZY, Bramlage WJ (1992) Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts. J Agric Food Chem 40:1566–1570
Gao FK, Dai CC, Liu XZ (2010) Mechanisms of fungal endophytes in plant protection against pathogens. Afr J Microbiol Res 4:1346–1351
Gao XN, Han QM, Chen YF, Qin HQ, Huang LL, Kang ZS (2014) Biological control of oilseed rape Sclerotinia stem rot by Bacillus subtilis strain Em7. Biocontrol Sci Tech 24:39–52
Ge LL, Zhang HY, Chen KP, Ma LC, Xu ZL (2010) Effect of chitin on the antagonistic activity of Rhodotorula glutinis against Botrytis cinerea in strawberries and the possible mechanisms involved. Food Chem 120:490–495
Hegedus DD, Rimmer SR (2005) Sclerotinia sclerotiorum: when “to be or not to be” a pathogen? FEMS Microbiol Lett 251:177–184
Kim KK, Kang JG, Moon SS, Kang KY (2000) Isolation and identification of antifungal N-butylbenzenesulphonamide produced by Pseudomonas sp. AB2. J Antibiot 53:131–136
Kumar S, Kaushik N, Edradaebel RA, Ebel R, Proksch P (2008) Endophytic fungi for pest and disease management. In: Ciancio A, Mukerji KG (eds) Integrated Management of Diseases Caused by Fungi, Phytoplasma and Bacteria. Springer Netherlands, Dordrecht, pp 365–387
Lu G (2003) Engineering Sclerotinia sclerotiorum resistance in oilseed crops. J Biotechnol 2:509–516
Morris DL (1948) Quantitative determination of carbohydrates with Dreywood’s anthrone reagent. Science 107:254–255
Muthukumar A, Eswaran A, Nakkeeran S, Sangeetha G (2010) Efficacy of plant extract and biocontrol agents against Pythium aphanidermatum inciting chilli damping-off. Crop Prot 29:1483–1488
Pan F, Liu ZQ, Chen Q, Xu YW, King H, Wu W (2016) Endophytic fungus strain 28 isolated from Houttuynia cordata possesses wide-spectrum antifungal activity. Braz J Microbiol 47:480–488
Park JH, Ji HP, Choi GJ, Lee SW, Jang KS, Choi YH, Cho KY, Kim JC (2003) Screening for antifungal endophytic fungi against six plant pathogenic fungi. Mycobiology 31:179–182
Park JH, Choi GA, Lee HB, Kim KM, Jung HS, Lee SW, Jang KS, Cho KY, Kim JC (2005) Griseofulvin from Xylaria sp. Strain F0010, an endophytic fungus of Abies holophylla and its antifungal activity against plant pathogenic fungi. J Microbiol Biotechnol 15:112–117
Petrini O, Sieber TN, Toti L, Viret O (1993) Ecology, metabolite production, and substrate utilization in endophytic fungi. Nat Toxins 1:185–196
Rakshith D, Santosh P, Satish S (2013) Isolation and characterization of antimicrobial metabolite producing endophytic Phomopsis sp. from Ficus pumila Linn. (Moraceae). Int J Chem Anal Sci 4:156–160
Rodrigues KF, Hesse M, Werner C (2000) Antimicrobial activities of secondary metabolites produced by endophytic fungi from Spondias mombin. J Basic Microbiol 40:261–267
Sang XX, Guo J, Bai LH (2014) Isolation, identification and analysis of secondary metabolites of multidrug-resistance inhibiting endophytic fungi of Salvia miltiorrhizal Bge. Chinese Journal of Applied and Environmental Biology 20:621–628
Shao X, Cheng S, Wang H, Yu D, Mungai C (2013) The possible mechanism of antifungal action of tea tree oil on Botrytis cinerea. J Appl Microbiol 114:1–8
Shen Y, Shen HM, Shi CY, Ong CN (1996) Benzene metabolites enhance reactive oxygen species generation in HL60 human leukemia cells. Hum Exp Toxicol 15:422–427
Tian J, Ban XQ, Zeng H, He JS, Chen YX, Wang YW (2012) The mechanism of antifungal action of essential oil from dill (Anethum graveolens L.) on Aspergillus flavus. PLoS One 7:e30147
Wang J, Xia XM, Wang HY, Li PP, Wang KY (2013) Inhibitory effect of lactoferrin against gray mould on tomato plants caused by Botrytis cinerea and possible mechanisms of action. Int J Food Microbiol 161:151–157
Zhu WJ, Wei W, Fu YP, Cheng JS, Xie JT, Li GQ, Yi XH, Kang ZS, Dickman MB, Jiang DH (2013) A secretory protein of necrotrophic fungus Sclerotinia sclerotiorum that suppresses host resistance. PLoS One 8:e53901
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This work was supported by the International Science and Technology Cooperation Program of China (No. 2015DFR31060).
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Supplementary Fig. S1
Influence of strain S4 extract on the number of sclerotia formation of Sclerotinia sclerotiorum. (a) Control without exposure to strain S4 extract; (b-f) Treatment with strain S4 extract at (b) 0.5 mg·ml−1, (c) 1.0 mg·ml−1, (d) 1.5 mg·ml−1, (e) 2.0 mg·ml−1 and (f) 2.5 mg·ml−1, respectively. (PNG 1637 kb)
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Huang, L., Li, F., Liu, R. et al. Antifungal activity of an endophytic strain of Phomopsis sp. on Sclerotinia sclerotiorum, the causal agent of Sclerotinia disease. J Plant Pathol 101, 521–528 (2019). https://doi.org/10.1007/s42161-018-00225-y
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DOI: https://doi.org/10.1007/s42161-018-00225-y