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Tara (Caesalpinia spinosa L.) tannin promotes proliferation and expression of hair growth-associated markers in human follicular dermal papilla cells

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Abstract

Hair loss is a distressing condition that may not be life-threatening but has an indisputable impact on psychological well-being of an individual. African plant resources have a great potential for use in hair growth promotion. Here, the effect of tara tannin from tara (Caesalpinia spinosa) pods on hair growth promotion was investigated in vitro using hair follicle dermal papilla cells (HFDPC). The noncytotoxic concentration of tara tannin was determined by subjecting HFDPCs to cytotoxicity assay. Then, ATP production was evaluated and quantitative polymerase chain reaction (qPCR) of hair growth promotion molecular markers was performed to determine the promotion effect on hair growth. Results showed that 5 µM tara tannin stimulated HFPDC proliferation, accompanied by an increase in ATP production. Fluorescent staining revealed an increase in ß-catenin in tara-tannin-treated cells. QPCR results confirmed that 5 µM tara tannin upregulated the expression of ß-catenin (CTNBB), alkaline phosphatase (tissue-nonspecific isozyme) (ALPL), neural cell adhesion molecule 1 (NCAM1), and fibroblast growth factor 1 (FGF1) in HFDPCs. These genes’ expression was upregulated in dermal papilla during the anagen phase of the hair cycle. DNA microarray analysis of tara-tannin-treated pigment cell B16F10 cells (72 h) revealed that cell cycle was one of the most significant signaling pathways modulated. Cell cycle analysis showed that cells were mostly in the G1 phase, consistent with HFDPCs during hair morphogenesis. The results of this study indicated that tara tannin may be used as treatment for alopecia by promoting hair growth.

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Authors and Affiliations

  1. Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba City, Ibaraki, 305-8587, Japan

    Myra O. Villareal & Hiroko Isoda

  2. Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Tennodai 1-1-1, Tsukuba City, Ibaraki, 305-8587, Japan

    Myra O. Villareal & Hiroko Isoda

  3. School of Integrative and Global Majors (SIGMA), University of Tsukuba, Tennodai 1-1-1, Tsukuba City, Ibaraki, 305-8587, Japan

    Myra O. Villareal, Meriem Bejaoui, Thanyanan Chaochaiphat, Kozo Sato & Hiroko Isoda

  4. Nano Innovation Laboratories Co., Ltd., Ninomiyamachi, Nakagun, Kanagawa, 259-0131, Japan

    Thanyanan Chaochaiphat

Authors
  1. Myra O. Villareal
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  2. Meriem Bejaoui
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  3. Thanyanan Chaochaiphat
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  4. Kozo Sato
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  5. Hiroko Isoda
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Correspondence to Hiroko Isoda.

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The authors declare no conflicts of interest.

Ethics approval

Human hair follicle dermal papilla cells (HFDPCs) were purchased from Cell Application Inc. (Tokyo, Japan), while B16F10 cells were purchased from Riken Cell Bank (Tsukuba, Japan). The experiments performed were done in compliance with the University of Tsukuba’s guidelines for use of cell cultures. No animals were used in the study.

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Responsible Editor: Mohamed Ksibi.

This paper was selected from the Tunisia-Japan Symposium on Science, Society and Technology (TJASSST 2019), Sousse, Tunisia.

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41207_2021_273_MOESM1_ESM.pptx

Suppl Fig. 1. Photographs of B16F10 murine melanoma cells treated with 25 µM tara tannin for 24 h and 48 h, and in comparison with untreated (Control) cells. Photographs of the cells were taken using Leica DFC290 HD camera (Beckman Coulter, CA, USA) (PPTX 574 kb)

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Villareal, M.O., Bejaoui, M., Chaochaiphat, T. et al. Tara (Caesalpinia spinosa L.) tannin promotes proliferation and expression of hair growth-associated markers in human follicular dermal papilla cells. Euro-Mediterr J Environ Integr 6, 68 (2021). https://doi.org/10.1007/s41207-021-00273-4

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  • DOI: https://doi.org/10.1007/s41207-021-00273-4

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