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Comprehensive analysis of tara tannins by reversed-phase and hydrophilic interaction chromatography coupled to ion mobility and high-resolution mass spectrometry

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Abstract

Reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) methods hyphenated to diode array detection and ion mobility (IM) high-resolution mass spectrometry (HR-MS) were used for the analysis of gallic acid derivatives and gallotannins in a commercial tara extract. UV spectra combined with low and high-collision energy mass spectral data and known RP-LC elution orders allowed the identification of 45 isomeric gallic acid derivatives and gallotannins. The synergy between IM and UV data was found to provide a simple means to determine the number of depsidic bonds and thus to distinguish between positional isomers. IM also facilitated the assignment of individual isomeric species between HILIC and RP-LC separations. For the hydrolysable tannins present in tara, RP-LC provided superior resolution and specificity compared to HILIC. The results reported in this paper highlight the utility of IM in combination with optimised complementary chromatographic separations and HR-MS for the detailed qualitative analysis of hydrolysable tannins in complex mixtures of these compounds.

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Acknowledgements

The authors gratefully acknowledge Prof. A. Pizzi for providing the tannin sample.

Funding

This study was financially supported by Sasol (SASOL Chair grant to HP, collaborative grant to AdV) and the National Research Foundation (NRF, grant 98897 to AdV, bursary to PV).

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  1. Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa

    Pieter Venter, Harald Pasch & André de Villiers

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  1. Pieter Venter
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  2. Harald Pasch
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  3. André de Villiers
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Correspondence to André de Villiers.

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Published in the topical collection Close-Up of Current Developments in Ion Mobility Spectrometry with guest editor Gérard Hopfgartner.

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Venter, P., Pasch, H. & de Villiers, A. Comprehensive analysis of tara tannins by reversed-phase and hydrophilic interaction chromatography coupled to ion mobility and high-resolution mass spectrometry. Anal Bioanal Chem 411, 6329–6341 (2019). https://doi.org/10.1007/s00216-019-01931-x

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