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Propagation through juvenile shoot cuttings in difficult-to-root Dalbergia latifolia – examining role of endogenous IAA in adventitious rooting

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Abstract

Dalbergia latifolia Roxb. is a premium-quality high-priced timber species of poor rooting ability restricting the propagation of its superior germplasm. Non-sustainable exploitation of the species left its natural population dwindling and vulnerable (VU) status. Despite its high market value, its occurrence is sporadic in natural forests and plantations are ‘rare’ in central India. With the aim of improvement in rooting ability, the seedling progeny of 10 selected superior germplasm of D. latifolia was raised for propagation through juvenile shoot cuttings. Progeny was hedged yearly in the last week of April and juvenile shoot cuttings were used for the experiment. With the varying rooting ability of progenies, an average of 35.83% adventitious rooting was achieved in shoot cuttings from 1 to 2 year-old progenies with basal dip treatment of 2.0mM IAA which gradually decline in successive years. 4–5 years old progenies exhibited 30.33% adventitious rooting with basal dip treatment of 5.0mM IAA. Further reduction in adventitious rooting was recorded in cuttings from 8 to 9 years and 10–11 years old progenies that exhibited 26.33% and 27.00% adventitious rooting with basal dip treatment of 5.0mM IAA + 1.0mM Boric Acid. Endogenous indole-3-acetic acid (IAA) available at the base of cuttings exhibited a non-significant relationship with the rooting ability for progenies of different ages. Besides, transport and availability of exogenously applied rooting hormone regulate the lateral movement of endogenously available IAA to the target cells in the pericyclic region substantially promoting the induction of adventitious rooting. Therefore, the use of juvenile shoot cuttings from progenies of selected trees was the most efficient procedure for the production of quality planting stock of Dalbergia latifolia.

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Data available with the senior author and planting material available in the nursery and field.

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Bartlett test used.

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Acknowledgements

The senior author is grateful to the Indian Council of Forestry Research and Education, Dehradun for financial assistance by award of projects [Project ID: 131/TFRI-2008/GEN-1(17) and 227/TFRI/2016/Gen-2(33)]

Funding

Study was conducted under the projects funded by the Indian Council of Forestry Research and Education, Dehradun [Project ID: 131/TFRI-2008/GEN-1(17) and 227/TFRI/2016/Gen-2(33)]

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  1. Genetics and Tree Improvement Division, Tropical Forest Research Institute PO-RFRC, Mandla Road, 482 021, Jabalpur, M.P, India

    Pramod Kumar, Pawan Kumar Patel & M. K. Sonkar

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  1. Pramod Kumar
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  2. Pawan Kumar Patel
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  3. M. K. Sonkar
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Senior author (PK) planned and executed experiments and IAA assay, analyzed data and prepared manuscript, PKP and MKS supervised experiments, helped in data collection and laboratory estimation of IAA and provided technical inputs in manuscript writing.

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Correspondence to Pramod Kumar.

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Kumar, P., Patel, P.K. & Sonkar, M.K. Propagation through juvenile shoot cuttings in difficult-to-root Dalbergia latifolia – examining role of endogenous IAA in adventitious rooting. Plant Physiol. Rep. 27, 242–249 (2022). https://doi.org/10.1007/s40502-022-00664-x

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