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Physiological analysis of C3 rice [Oryza sativa (L.)] and C4 millet [Setaria italica (L.)] to identify photosynthetically efficient plants

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Abstract

The study was undertaken with the aim of developing a reliable and rapid screening protocol to identify crop plants with higher photosynthetic efficiencies. The vascular architecture and physiological functions of the leaves of C3 and C4 photosynthetic genetic model systems were compared. Vein densities, leaf chlorophyll index, Chlorophyll a fluorescence and gas exchange parameters were correlated with photosynthetic efficiency to develop an efficient protocol. The photosynthetic rates of Setaria italica were almost double when compared with the C3 plants. The higher photosynthetic efficiency of Setaria italica was supported by high photosynthetic rates coupled with moderate to high stomatal conductance and low transpiration rates. Setaria italica showed a significant increase in PSII efficiency as the growth progressed, while no significant changes in Fv/Fm values were observed in Oryza sativa genotypes. The distribution of SPAD index values among the rice genotypes during the entire growth period did not exceed 34.35. Setaria italica genotypes exhibited a denser arrangement of SLV’s, almost three times higher than Oryza sativa, with an interveinal distance of 0.317–0.329 mm. Denser networks of small longitudinal veins, higher SPAD indices and PSII efficiency were key traits to look for photosynthetic efficiency in crop plants.

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References

  • Dengler, N. G., Dengler, R. E., Donnelly, P. M., & Hattersley, P. W. (1994). Quantitative leaf anatomy of C3and C4 grasses (Poaceae): Bundle sheath and mesophyll surface area relationships. Annals of Botany, 73, 241–255.

    Article  Google Scholar 

  • Doust, A. N., Kellogg, E. A., Devos, K. M., & Bennetzen, J. L. (2009). Foxtail millet: A sequence-driven grass model system. Plant Physiology, 149, 137–141.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Earl, H. J., & Tollenaar, M. (1999). Using chlorophyll fluorometry to compare photosynthetic performance of commercial maize (Zea mays L.) hybrids in the field. Field Crops Research, 61, 201–210.

    Article  Google Scholar 

  • Edwards, E. J., & Smith, S. A. (2010). Phylogenetic analyses reveal the shady history of C4 grasses. Proceedings of National Academy of Sciences, 107, 2532–2537.

    Article  Google Scholar 

  • Flexas, J., Diaz-Espejo, A., Galmés, J., Kaldenhoff, R., Medrano, H., & RibasCarbo, M. (2007). Rapid variations of mesophyll conductance in response to changes in CO2 concentration around leaves. Plant Cell and Environment, 30, 1284–1298.

    Article  CAS  Google Scholar 

  • Giuliani, Rita, Koteyeva, Nuria, Voznesenskaya, Elena, Evans, M. A., Cousins, A. B., & Edwards, G. E. (2013). Coordination of leaf photosynthesis, transpiration and structural traits in rice and wild relatives (genus Oryza). Plant Physiology, 162, 1632–1651.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Gomez, A. K., & Gomez, A. A. (1984). Statistical procedures for agricultural research. Hoboken: Wiley.

    Google Scholar 

  • Kim, E. J., Sa, K. J., Park, K. C., & Lee, J. K. (2012). Study of genetic diversity and relationships among accessions of foxtail millet [Setaria italica (L.) P. Beauv.] in Korea, China, and Pakistan using SSR markers. Genes and Genomes, 34, 529–538.

    Article  CAS  Google Scholar 

  • Kubien, D. S., von Caemmerer, S., Furbank, R. T., & Sage, R. F. (2003). C4 photosynthesis at low temperature. A study using transgenic plants with reduced amounts of Rubisco. Plant Physiology, 132, 1577–1585.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kusumi, K. (2012). Increased leaf photosynthesis caused by elevated stomatal conductance in a rice mutant deficient in SLAC1, a guard cell anion channel protein. Journal of Experimental Botany, 63(15), 5635–5644.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Li, P., & Brutnell, T. P. (2011). Setaria viridis and Setaria italica, model genetic systems for the Panicoid grasses. Journal of Experimental Botany, 62, 3031–3037.

    Article  PubMed  CAS  Google Scholar 

  • Lu, C., & Zhang, J. (1999). Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants. Journal of Experimental Botany, 50(336), 1199–1206.

    Article  CAS  Google Scholar 

  • Markwell, J., Osterman, J. C., & Mitchell, J. L. (1995). Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynthesis Research, 46, 467–472.

    Article  PubMed  CAS  Google Scholar 

  • Netto, A. T., Campostrini, E., Goncalves de Oliveira, J., & Bressan-Smith, R. E. (2005). Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves. Scientia Horticulturae, 104, 199–209.

    Article  CAS  Google Scholar 

  • Parry, M. A. J., Reynolds, M., Salvucci, M. E., Raines, C., Andralojc, P. J., Zhu, X. G., et al. (2011). Raising yield potential of wheat. II. Increasing photosynthetic capacity and efficiency. Journal of Experimental Botany, 62, 453–467.

    Article  PubMed  CAS  Google Scholar 

  • Paterson, A. H., Bowers, J. E., Bruggmann, R., Dubchak, I., & Grimwood, J. (2009). The Sorghum bicolor genome and diversification of grasses. Nature, 457, 551–556.

    Article  PubMed  CAS  Google Scholar 

  • Silva, M. A., Jifon, J. L., Da Silva, J. A. G., & Sharma, V. (2007). Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane. Brazilian Journal of Plant Physiology, 19, 193–201.

    Article  Google Scholar 

  • Smillie, I. R.A. (2012). Analysis of leaf morphology and photosynthesis in Deletion mutants of rice (Oryza sativa L.). Ph.D. thesis, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, Le12 5rd.

  • Snedecor, G. W., & Cochran, W. G. (1994). Statistical methods. Des Moines: Iowa State University.

    Google Scholar 

  • Ueno, O., Kawano, Y., Wakayama, M., & Takeda, T. (2006). Leaf vascular systems in C3 and C4 grasses: A two-dimensional analysis. Annals of Botany, 97, 611–621.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ueno, O., & Sentoku, N. (2006). Comparison of leaf structure and photosynthetic characteristics of C3 and C4 Alloteropsis semialata subspecies. Plant Cell and Environment. https://doi.org/10.1111/j.1365-3040.2005.01418.x.

    Article  Google Scholar 

  • Uprety, D. C., Sunita, K., Dwivedi, N., & Rajat, M. (2000). Effect of elevated CO2 on the growth and yield of rice. Indian Journal of Plant Physiology, 5, 105–107.

    Google Scholar 

  • Vijayalakshmi, P., Vishnu Kiran, T., Venkateswara Rao, Y., Srikanth, B., Subhakara Rao, I., Sailaja, B., et al. (2013). Physiological approaches for increasing nitrogen use efficiency in rice. Indian Journal of Plant Physiology, 18(3), 208–222.

    Article  Google Scholar 

  • Vivitha, P., & Vijayalakshmi, D. (2015). Minor millets as model system to study C4 photosynthesis—A review. Agricultural Reviews, 36(4), 296–304.

    Article  Google Scholar 

  • Wang, C., Guo, Longyun, Li, Y., & Wang, Z. (2012). Systematic comparison of C3 and C4 plants based on metabolic network analysis. Systems Biology, 6, 1–14.

    CAS  Google Scholar 

  • Weng, J. H., & Lai, M. F. (2005). Estimating heat tolerance among plant species by two chlorophyll fluorescence parameters. Photosynthetica, 43, 439–444.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, India

    D. Vijayalakshmi

  2. Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India

    M. Raveendran

Authors
  1. D. Vijayalakshmi
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  2. M. Raveendran
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Correspondence to D. Vijayalakshmi.

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Vijayalakshmi, D., Raveendran, M. Physiological analysis of C3 rice [Oryza sativa (L.)] and C4 millet [Setaria italica (L.)] to identify photosynthetically efficient plants. Ind J Plant Physiol. 23, 193–200 (2018). https://doi.org/10.1007/s40502-018-0373-9

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