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In vitro propagation and ex vitro rooting of Aerva lanata (L.) Juss. ex Schult.: a rare medicinal plant

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Abstract

The present study describes an improved and reproducible in vitro regeneration system for Aerva lanata using nodal segment explants obtained from a mature 2 years old plant. Direct organogenesis of shoots from nodal segments was achieved by culturing on Murashige and Skoog (MS) medium supplemented with 1.5 mg l−1 6-benzylaminopurine (BAP). On this media combination, 8.7 ± 0.21 shoots per explant were induced. Shoots multiplication was extensively influenced by the type and the concentrations of plant growth regulators used, repeated transfer of mother explants and the sub-culturing of in vitro regenerated shoot clumps on fresh medium. Maximum numbers of shoots (49.39 ± 1.24) was observed on MS medium augmented with 1.0 mg l−1 BAP and 0.2 mg l−1 α-naphthalene acetic acid. Full, half and one-fourth strength of MS medium with different concentrations of auxins was tested for in vitro root formation. One fourth strength MS medium with 2.0 mg l−1 indole-3 butyric acid (IBA) was optimum for in vitro root induction (36.9 ± 0.72 roots per shoot). Experiments on ex vitro rooting were conducted to shorten the regeneration pathway and observed highest percentage (100 %) of rooting from the cut ends of the shoots with 200 mg l−1 IBA. About 12.6 ± 0.30 roots were induced from the shoots using this method. The well rooted plantlets were acclimatized successfully in Soilrite under the greenhouse conditions with 92 % survival rate.

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Acknowledgments

Authors are grateful to the University Grants Commission, New Delhi, Government of India and Department of Science, Technology and Environment, Government of Puducherry for providing financial support to our laboratory as Major Research Project and Grant–In-Aid Scheme respectively.

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Authors and Affiliations

  1. Biotechnology Laboratory, Department of Plant Science, Mahatma Gandhi Government Arts College, Mahe, Puducherry, India

    Mahipal S. Shekhawat & M. Manokari

  2. Department of Botany, Kanchi Mamunivar Centre for Postgraduate Studies, Puducherry, India

    J. Revathi

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  1. Mahipal S. Shekhawat
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  2. M. Manokari
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  3. J. Revathi
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Correspondence to Mahipal S. Shekhawat.

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Shekhawat, M.S., Manokari, M. & Revathi, J. In vitro propagation and ex vitro rooting of Aerva lanata (L.) Juss. ex Schult.: a rare medicinal plant. Ind J Plant Physiol. 22, 40–47 (2017). https://doi.org/10.1007/s40502-016-0248-x

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  • DOI: https://doi.org/10.1007/s40502-016-0248-x

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