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Effects of leaf age on chlorophyll fluorescence and antioxidant enzymes activity in winter rapeseed leaves under cold acclimation conditions

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Abstract

Cold acclimation-dependent physiological changes were investigated through photosynthetic activity and some antioxidant enzymes in two winter oilseed rape cultivars (Brassica napus L. ssp. oleifera cvs. Eurol and Hansen) on the basis of leaf age. Chlorophyll fluorescence measurements demonstrated that cold acclimation caused decreased photosynthetic activity in Eurol and Hansen as the leaf age increased. Photosynthetic pigment content declined in both cultivars under low temperature irrespective of leaf age. In Eurol, however, relatively higher total carotenoid content than Hansen may provide a protection against photooxidative damage. Superoxide dismutase, ascorbate peroxidase and glutathione reductase activity in the cold-acclimated leaves of Hansen significantly increased, indicating an effective ascorbate–glutathione cycle activity. In Eurol, however, ascorbate peroxidase and glutathione reductase activity in the cold-acclimated younger leaves lowered and hydrogen peroxide detoxifying system collapsed. As a result, decreased photosynthetic activity in the young leaves of Hansen under low temperature could be manifested as a metabolic response that downregulate photosynthesis to attain cold-acclimated state. In the case of Eurol, cold acclimation process caused oxidative stress. In summary, the changes in the chlorophyll fluorescence parameters in the young leaves of Hansen are derived from some metabolic regulation as a result of cold acclimation, whereas in Eurol, it is due to oxidative stress triggered by low temperature. Leaf maturation mitigated the adverse effects of low temperature in the leaves of Eurol and Hansen.

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Acknowledgements

This study was funded by Hacettepe University Scientific Research Fund (Grant Number: 0201601005).

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Science, Sakarya University, Esentepe, Sakarya, Turkey

    Ali Doğru

  2. Department of Biology, Faculty of Science, Hacettepe University, Beytepe, Ankara, Turkey

    Hüsnü Çakirlar

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  1. Ali Doğru
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  2. Hüsnü Çakirlar
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by AD. The first draft of the manuscript was written by AD, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ali Doğru.

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Doğru, A., Çakirlar, H. Effects of leaf age on chlorophyll fluorescence and antioxidant enzymes activity in winter rapeseed leaves under cold acclimation conditions. Braz. J. Bot 43, 11–20 (2020). https://doi.org/10.1007/s40415-020-00577-9

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  • DOI: https://doi.org/10.1007/s40415-020-00577-9

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