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Response of photosynthetic capacity and antioxidative system of chloroplast in two wucai (Brassica campestris L.) genotypes against chilling stress

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Abstract

Chilling stress during the growing season could cause a series of changes in wucai (Brassica campestris L.). WS-1 (chilling-tolerant genotype) and Ta2 (chilling-sensitive genotype) were sampled in present study to explore the chilling tolerance mechanisms. Our results indicated that photosynthetic parameters exhibited lower level in Ta2 than in WS-1 under chilling stress. The rapid chlorophyll fluorescence dynamics curve showed that chilling resulted in a greater inactivation of photosystem II reaction center in Ta2. Reactive oxygen species and malondialdehyde content of chloroplast in Ta2 were higher than WS-1. The ascorbate–glutathione cycle in chloroplast of WS-1 played a more crucial role than Ta2, which was confirmed by higher activities of antioxidant enzymes including Ascorbate peroxidase, Glutathione reductase, Monodehydroascorbate reductase and Dehydroascorbate reductase and higher content of AsA and GSH. In addition, the ultrastructure of chloroplasts in Ta2 was more severely damaged. After low temperature stress, the shape of starch granules in Ta2 changed from elliptical to round and the volume became larger than that of WS-1. The thylakoid structure of Ta2 also became dispersed from the original tight arrangement. Combined with our previous study under heat stress, WS-1 can tolerant both chilling stress and heat stress, which was partly due to a stable photosynthetic system and the higher active antioxidant system in plants, in comparison to Ta2.

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Acknowledgements

This work was funded by Major Science and Technology Projects of Anhui Province, China (17030701013); National Key R & D Program of China (2017YFD0101803), National Natural Science Foundation of China (No. 31701910).

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  1. Jie Wang and Rou Fanga have contributed equally to this work.

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  1. Vegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China

    Jie Wang, Rou Fang, Lingyun Yuan, Guoqin Yuan, Mengru Zhao, Shidong Zhu, Jinfeng Hou, Guohu Chen & Chenggang Wang

  2. Provincial Engineering Laboratory for Horticultural Crop Breeding of Anhui, Hefei, 230036, China

    Jie Wang, Rou Fang, Lingyun Yuan, Mengru Zhao, Shidong Zhu, Jinfeng Hou, Guohu Chen & Chenggang Wang

  3. Wanjiang Vegetable Industrial Technology Institute, Maanshan, 243000, Anhui, China

    Lingyun Yuan, Shidong Zhu, Jinfeng Hou & Chenggang Wang

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WC, WJ, FR and YL designed the experiment. WJ, FR and YL carried out the experiments and wrote the manuscript. CG and HJ supervised the study and YG, ZM helped perform the experiments. All authors have read and approved the final manuscript.

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Wang, J., Fang, R., Yuan, L. et al. Response of photosynthetic capacity and antioxidative system of chloroplast in two wucai (Brassica campestris L.) genotypes against chilling stress. Physiol Mol Biol Plants 26, 219–232 (2020). https://doi.org/10.1007/s12298-019-00743-8

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