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Effects of low nocturnal temperature on photosynthetic characteristics and chloroplast ultrastructure of winter rapeseed

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Abstract

We investigated the effects of low nocturnal temperature on photosynthetic apparatus of winter rapeseed (Brassica campestris L.). An artificial climate chamber was used to simulate the effects of low nocturnal temperature on seedling and stomatal morphology, chloroplast ultrastructure, photosynthetic parameters, and dry matter distribution and accumulation in two winter rapeseed cultivars, Longyou-7 (ultra coldresistant) and Tianyou-2 (weak cold resistance). Compared with those at diurnal/nocturnal temperatures of 20°/10°C (control), rapeseed seedlings at 20°/5°C had increased leaf chlorophyll content, deepened green leaf color, decreased stomatal conductance (G s), intercellular CO2 concentration (C i), and photosynthetic rate (P n), and improved root/shoot ratio; the majority of stomata remained open in Longyou-7 while those in Tianyou-2 were mostly closed or semi-closed. At diurnal/nocturnal temperatures of 20°/–5°C, rapeseed seedlings had decreased leaf chlorophyll content with increased C i but decreased G s and P n; Tianyou-2 exhibited ruptured chloroplast membrane, dissolved grana, broken stroma lamella, and decreased root/shoot ratio, whereas Longyou-7 had chloroplasts retaining partial structure of grana with a small amount of starch granules in guard cells. Low nocturnal temperature damaged the photosynthetic membrane of chloroplasts and reduced P n in the leaves of winter rapeseed influencing photosynthetic processes in this crop. The reduction of P n was mainly related to stomatal limitation at diurnal/nocturnal temperatures of 20°/5°C and non-stomatal limitation at diurnal/nocturnal temperatures of 20°/–5°C.

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Abbreviations

DW:

dry weight

FW:

fresh weight

G s :

stomatal conductance

C i :

intercellular CO2 concentration

P n :

photosynthetic rate

T r :

transpiration rate

PEPC:

phosphoenolpyruvate carboxylase

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Authors and Affiliations

  1. Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, China

    Z. G. Liu, W. C. Sun, Y. N. Zhao, X. C. Li, Y. Fang, J. Y. Wu, X. C. Zeng, N. N. Yang, Y. Wang & L. He

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  1. Z. G. Liu
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  2. W. C. Sun
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  3. Y. N. Zhao
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  4. X. C. Li
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  5. Y. Fang
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  6. J. Y. Wu
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  8. N. N. Yang
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  9. Y. Wang
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  10. L. He
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Correspondence to Z. G. Liu.

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Liu, Z.G., Sun, W.C., Zhao, Y.N. et al. Effects of low nocturnal temperature on photosynthetic characteristics and chloroplast ultrastructure of winter rapeseed. Russ J Plant Physiol 63, 451–460 (2016). https://doi.org/10.1134/S1021443716040099

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  • DOI: https://doi.org/10.1134/S1021443716040099

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