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Cytotoxic Effect of (Z)-Ethylidene-4,6-Dimethoxycoumaran-3-One Isolated from Pogostemon quadrifolius (Benth.) on PC-3 and DU-145 Prostate Cancer Cells

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The study was conducted to identify the antiproliferative property and the mode of action of (Z)-ethylidene-4,6-dimethoxycoumaran-3-one (EDC), a novel compound isolated from the plant leaves of Pogostemon quadrifolius (Benth.), against prostate cancer cell lines PC-3 and DU-145. EDC inhibited the proliferation of PC-3 and DU-145 cell lines at micro molar concentrations. EDC strongly inhibited the clonogenic propagation of prostate cancer cells. EDC arrests the prostate cancer cells at G2/M phase of the cell cycle and then eventually leads to apoptosis, which was confirmed by DNA fragmentation assay and poly (ADP-ribose) polymerase cleavage western blot analysis. At sub-lethal concentrations, EDC inhibited the migration of PC-3 by 87.1% and DU-145 by 68.0%. Thus, the present study revealed the in vitro cytotoxic and anti-metastatic property of EDC against PC-3 and DU-145 cancer cells.

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Acknowledgements

The authors acknowledge the University of Calicut and DBT, Government of India for providing research facilities and financial support. This research is a part of Ph.D thesis of Sanith Cheriyamundath.

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Authors and Affiliations

  1. Department of Biotechnology, University of Calicut, Malappuram, Kerala, 673635, India

    Sanith Cheriyamundath, Rahul Raghavan, Deepika Vinod, K. B. Megha & Joseph Madassery

  2. Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam, Kerala, 690525, India

    Asoke Banerji

  3. Molecular Structure Analysis, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120, Heidelberg, Germany

    Karel D. Klika

  4. Division of Preventive Oncology, National Center for Tumor Diseases (NCT), German Cancer Research Center (DKFZ), Im Neuenheimer Feld 460, 69120, Heidelberg, Germany

    Robert W. Owen

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  1. Sanith Cheriyamundath
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  2. Rahul Raghavan
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  3. Deepika Vinod
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  4. K. B. Megha
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  7. Robert W. Owen
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  8. Joseph Madassery
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Correspondence to Joseph Madassery.

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Additional information

Significant statement (Z)-ethylidene-4,6-dimethoxycoumaran-3-one (EDC), is a novel compound isolated from the plant leaves of Pogostemon quadrifolius (Benth.). Since it is a new compound not much studies are conducted to identify its biological activities. In the present study, the authors identified the in vitro cytotoxic and anti-metastatic property of EDC against the prostate cancer cell lines such as PC-3 and DU-145.

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Cheriyamundath, S., Raghavan, R., Vinod, D. et al. Cytotoxic Effect of (Z)-Ethylidene-4,6-Dimethoxycoumaran-3-One Isolated from Pogostemon quadrifolius (Benth.) on PC-3 and DU-145 Prostate Cancer Cells. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 1581–1588 (2018). https://doi.org/10.1007/s40011-017-0902-8

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  • DOI: https://doi.org/10.1007/s40011-017-0902-8

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