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Haplotypic Diversity and Virulence of Xanthomonas oryzae pv. oryzae Population Infecting the First Superfine Aromatic Basmati Hybrid

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is a serious constraint to basmati rice production. The increasingly evident outbreaks of BB in recent years necessitate an immediate search for ecology-conscious, cost-effective and durable management strategy. Given the context, this investigation aimed to generate knowledge about the haplotypic and pathogenic variability of Xoo population severely affecting Pusa Rice Hybrid 10 (PRH-10), world’s first superfine aromatic basmati hybrid. Seven haplotypes were detected from the experimental field applying two PCR-based assays, i.e. repetitive sequence-based polymerase chain reaction and insertion sequence based PCR. The experimental results revealed greater genetic heterogeneity in the Xoo population with high value of total haplotypic diversity (H T  = 0.79). In addition, based on the virulence assay, the genetic heterogeneity corresponded to the presence of four pathotypes. Importantly, the substantial line × strain interactions was significant (p ≤ 0.01), which confirmed the host specificity in the system. In parallel, it was noted that most of the key resistance genes except Xa21, deployed in the rice breeding program at International Rice Research Institute were overcome by the Xoo strains, thus underscoring the relevance of this gene in resistance breeding. Therefore, incorporating Xa21 alone, or in combination with xa13 or xa5 into the parental lines, using various modern genomics-assisted approaches might be the potential fast-track strategy to breed PRH-10 with remarkably enhanced BB resistance. The present investigation provides a molecular framework for future epidemiological studies and is likely to assist rice breeders to expedite basmati rice improvement.

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Acknowledgments

The authors are grateful to International Rice Research Institute, Manila, Philippines for providing the seeds of near-isogenic lines. They also thank the reviewers for critical reading and valuable suggestions regarding the manuscript. The authors declare that there are no conflicts of interest.

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Author notes

  1. Shailesh Pandey

    Present address: Forest Protection Division, Rain Forest Research Institute, P.O. Box-136, Deovan, Jorhat, Assam, 785001, India

Authors and Affiliations

  1. Department of Plant Pathology, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India

    Shailesh Pandey & J. Kumar

  2. Department of Plant Breeding, Indian Institute of Pulse Research, Kanpur, India

    Abhishek Bohra

  3. National Research Centre on Plant Biotechnology, New Delhi, 110012, India

    Balwant Singh

  4. Regional Station, Directorate of Wheat Research (ICAR), Flowerdale, Shimla-2, HP, India

    Pramod Prasad

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  1. Shailesh Pandey
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Correspondence to Shailesh Pandey.

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Pandey, S., Bohra, A., Singh, B. et al. Haplotypic Diversity and Virulence of Xanthomonas oryzae pv. oryzae Population Infecting the First Superfine Aromatic Basmati Hybrid. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 1005–1014 (2017). https://doi.org/10.1007/s40011-015-0675-x

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  • DOI: https://doi.org/10.1007/s40011-015-0675-x

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