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Transferability of SSR Markers of Brassica sp. to Some Popular Varieties of Brassica Juncea

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

A set of 124 simple sequence repeat (SSR) markers previously developed for various Brassica sp. including B. nigra, B. oleracea, B. napus and B. rapa has been tested for cross-transferability in twelve popular varieties of B. juncea. Only 81 SSRs produced clear and distinguishable amplification products, while rest 43 primer pairs did not show any amplification in any of the genotypes of B. juncea. Amplified allele sizes ranged from 40 to 410 bp and the number of alleles per locus ranged from 1 to 6, with an average of 2.17 alleles/locus. Out of these 81 SSR primer pairs, the highest frequency (97.5 %) of cross-transferability was recorded for Indian mustard variety Kranti, where 79 markers were found transferable, while variety Vasundhara showed the lowest frequency (51.8 %) of cross-amplification with 42 markers only. Thirty two primer pairs were found to be transferable to all the 12 varieties tested. Overall, polymorphism percentage of the transferred markers was recorded to be 71.6 %. The Unweighted Pair Group Method with Arithmetic Mean dendrogram based on Jaccard’s similarity coefficient divided all the twelve varieties into five clusters. The transferability of these SSR markers indicates a high level of conserved sequences in the SSR flanking regions across species of the genus Brassica. High percentage of cross-transferability of these SSR markers to B. juncea cultivars suggested the possibility of using these markers for various applications in genetics and genomics of B. juncea.

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Acknowledgments

The authors sincerely thank the Director, DRMR, Bharatpur, Rajasthan for providing all the necessary facilities required to carry out this work. They are also grateful to Dr. S.S. Banga, National Professor, ICAR (Plant Breeding and Genetics), PAU, Ludhiana, Punjab for providing B. nigra-derived SSR markers.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Directorate of Rapeseed-Mustard Research, Bharatpur, Rajasthan, 321 303, India

    Ajay Kumar Thakur, K. H. Singh, Lal Singh, J. Nanjundan & Dhiraj Singh

  2. National Bureau of Plant Genetic Resources, New Delhi, 110 012, India

    M. K. Rana

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  1. Ajay Kumar Thakur
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  2. K. H. Singh
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  3. Lal Singh
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  4. J. Nanjundan
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  5. M. K. Rana
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  6. Dhiraj Singh
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Correspondence to Ajay Kumar Thakur.

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Thakur, A.K., Singh, K.H., Singh, L. et al. Transferability of SSR Markers of Brassica sp. to Some Popular Varieties of Brassica Juncea . Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 85, 1001–1010 (2015). https://doi.org/10.1007/s40011-014-0486-5

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  • DOI: https://doi.org/10.1007/s40011-014-0486-5

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