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Quantitative and Qualitative Aspects of Soluble and Ionically Wall-Bound Peroxidases of EMS-Mutagenized Durum Wheat Lines (Triticum durum), as Tested for Yield at Drought Conditions

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Abstract

In order to characterize the response to drought of 19 ethyl methanesulfonate (EMS)-mutagenized lines of durum wheat (Triticum durum Desf.) derived from the variety CHAM-1 and cultivated in Morocco, growth (plant height, PLH) and yield (grain number per spike, GNS) parameters were followed for different lines and CHAM-1 variety. All were cultivated under well-watered (C) and drought conditions (T). In parallel, quantitative and qualitative aspects of leaf peroxidases (EC 1.11.1.7) were analyzed. When compared with C plant lines, the corresponding T lines showed decreases of 9–38% and 1–78%, respectively, for PLH and GNS parameters. A positive correlation was found between PLH and the yield as GNS (r2 = 0.885). When expressed as units mg−1 proteins, soluble peroxidases (SPOX) were less represented than the ionically wall-bound peroxidases (IPOX) in all mutagenized wheat lines, with 22.1% and 46.2% as respective coefficients of variation for drought-stressed lines. Drought-induced reduction in grain yields for all lines correlated positively with percentages of IPOX exhibited by stressed plants (r2 = 0.68). In addition, Averages of drought-induced decreases in GNS, calculated for five groups of mutagenized lines, were found to be positively correlated with their respective percentages of IPOX (r2 = 0.906). Drought-adapted lines showed decreases in IPOX. When subjected to electrophoresis using 6–15% polyacrylamide gradient gels, resolved IPOX of cereal line groups of limited drought-induced reduction in GNS exhibited low reactivity on native gels. These data support the hypothesis that IPOX are indicators of the sensitivity of wheat lines to drought conditions. Possible mechanisms of POX involvement in wheat resistance to drought and developing appropriate biochemical tests to screen variability and drought adaptation of EMS mutants are discussed in this study.

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Acknowledgements

We acknowledge staff of the National Institute of Agronomic Research (INRA) at Rabat, Morocco, for help in plant culture.

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Authors and Affiliations

  1. Laboratory of Biochemistry and Plant Biotechnology, Faculty of Science Semlalia, Cadi Ayyad University, Marrakech, Morocco

    I. Kahama, K. Kohaich & M. Baaziz

  2. Biotechnology Research Unit, Agronomic Research Center, INRA, Rabat, Morocco

    I. Kahama & M. Labhilili

Authors
  1. I. Kahama
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  2. K. Kohaich
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  3. M. Baaziz
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  4. M. Labhilili
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Correspondence to M. Baaziz.

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This work is supported by Cadi Ayyad University, Marrakech, Morocco, accreditation 2016 and the National Institute of Agronomic Research (INRA), Morocco.

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Kahama, I., Kohaich, K., Baaziz, M. et al. Quantitative and Qualitative Aspects of Soluble and Ionically Wall-Bound Peroxidases of EMS-Mutagenized Durum Wheat Lines (Triticum durum), as Tested for Yield at Drought Conditions. Agric Res 8, 148–157 (2019). https://doi.org/10.1007/s40003-018-0361-3

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