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Comparison of resistance to drought of three bean cultivars

  • Published:
Biologia Plantarum

Abstract

The aim of the present work was to evaluate oxidative stress and plant antioxidant system of three contrasting bean (Phaseolus vulgaris L.) genotypes in the response to drought. Drought was imposed 14 d after emergence, by withholding water, until leaf relative water content reached 65 %. Water stress increased lipid peroxidation (LPO), membrane injury index, H2O2 and OH⋅ production in leaves of stressed plants. Activities of the antioxidative enzymes superoxide dismutase (SOD) and ascorbate peroxidase (APOX) increased significantly under water stress in all the studied cultivars, while catalase (CAT) increased in cvs. Plovdiv 10 and Prelom, but decreased in cv. Dobrudjanski ran. Furthermore cv. Plovdiv 10 which had the highest APOX and CAT activities also showed the lowest increase in H2O2 and OH⋅ production and LPO while cv. Dobrudjanski ran showed the lowest increases (and often the lowest values) in the antioxidant enzyme activities and the highest increases of H2O2 and OH⋅ production, and LPO. On the basis of the data obtained we could specify cv. Plovdiv 10 and cv. Prelom as drought tolerant and cv. Dobrudjanski ran as a drought sensitive.

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Abbreviations

APOX:

ascorbate peroxidase

CAT:

catalase

I %:

membrane injury index

LPO:

lipid peroxidation

MDA:

malondialdehyde

PPFD:

photosynthetic photon flux density

ROS:

reactive oxygen species

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Department of Plant Physiology and Biochemistry, Agricultural University, 4000, Plovdiv, Bulgaria

    Z. S. Zlatev

  2. Grupo de Disciplinas de Ecologia da Hidrosfera, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2825, Monte de Caparica, Portugal

    F. C. Lidon

  3. Departamento de Ciencias Naturais, Instituto de Investigacao Cientifica Tropical, Quinta do Marques, Av. Republica, 2784-505, Oeiras, Portugal

    J. C. Ramalho

  4. Institute of Plant Physiology “M. Popov”, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    I. T. Yordanov

Authors
  1. Z. S. Zlatev
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  2. F. C. Lidon
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  3. J. C. Ramalho
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  4. I. T. Yordanov
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Correspondence to Z. S. Zlatev.

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Zlatev, Z.S., Lidon, F.C., Ramalho, J.C. et al. Comparison of resistance to drought of three bean cultivars. Biol Plant 50, 389–394 (2006). https://doi.org/10.1007/s10535-006-0054-9

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  • DOI: https://doi.org/10.1007/s10535-006-0054-9

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