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Effects of light quality on growth, photosynthetic characteristics, and endogenous hormones in in vitro-cultured Lilium plantlets

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Abstract

Red (R, 640 nm) and blue (B, 464 nm) light are the most important regions in the spectrum absorbed by plants. Lilium plantlets cultured in vitro were exposed to 40 ± 2 μmol m−1 s−1 irradiance (spectral range: 400–800 nm) supplied by plant-growth fluorescent lamps (PGFL) under a 12-h light/12-dark photoperiod and cultured for 50 days under six light quality treatments: control (PGFL only); R (100% R); R:B = 8:2; R:B = 7:3; R:B = 5:5; B (100% B). Plant height and leaf number increased with increasing R light, and increasing B light inhibited fresh weight accumulation. The R:B = 7:3 treatment resulted in the highest pigment contents (chlorophyll b, carotenoids, total chlorophyll), photosynthetic indicator values (net photosynthetic rate, stomatal conductance, and intercellular CO2 concentration), and chlorophyll fluorescence parameters (maximal efficiency of photosystem II, relative quantum efficiency of photosystem II photochemistry, and photochemical quenching). The highest contents of total sugars, fructose, sucrose, and soluble protein in leaves were in the B-only treatment. At day 50, the trans-zeatin content was higher in the B-only treatment than in the R-only treatment, while the cis-zeatin content showed the opposite trend. The highest levels of indole acetic acid and gibberellin were in the R-only treatment, and the highest abscisic acid content was in the B-only treatment on day 50. We conclude that R light promoted stem elongation and leaf number, and B light increased the sugars and soluble protein contents in Lilium cultured in vitro. The R:B = 7:3 treatment was optimal for photosynthesis. These results will be useful for producing high-quality Lilium plants in vitro.

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Abbreviations

IAA:

Indole-3-acetic acid

NAA:

1-Naphthaleneacetic acid

6-BA:

6-Benzylaminopurine acid

MS:

Murashige-Skoog medium

ZT:

Zentin

GA3 :

Gibberellin

ABA:

Abscisic acid

Pn:

Net photosynthetic rate

gs :

Stomatal conductance

Ci:

Intercellular CO2 concentration

Fv/Fm :

Maximal PSII efficiency

ΦPSII:

Relative quantum efficiency of PSII photochemistry

qP:

Photochemical quenching

NPQ:

Non-photochemical quenching

MDA:

Malondialdehyde

SPS:

Sucrose phosphate synthase

SS:

Sucrose synthase

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Acknowledgements

We thank Liwen Bianji (Edanz) (www.liwenbianji.cn) for editing the English text of a draft of this manuscript.

Funding

This project was funded by Henan Industry-University Research Project (No. 162107000068) and National Scientific and Technological Achievements Transformation Project (No. 2012D0001018).

Author information

Author notes

  1. Wenqian Shang and Yinglong Song have contributed equally to this work.

Authors and Affiliations

  1. College of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou, 450002, China

    Wenqian Shang, Yinglong Song, Chunling Zhang, Liyun Shi, Yuxiao Shen, Xiaojuan Li, Zheng Wang & Songlin He

  2. College of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, 453003, China

    Songlin He

Authors
  1. Wenqian Shang
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  2. Yinglong Song
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  3. Chunling Zhang
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  4. Liyun Shi
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  5. Yuxiao Shen
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  6. Xiaojuan Li
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  7. Zheng Wang
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  8. Songlin He
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Contributions

WS and YS performed the experiments, analyzed data, and wrote the manuscript. CZ, XL, LS and YS carried out most of the experiments. ZW and SH started the experiments and revised the manuscript.

Corresponding authors

Correspondence to Zheng Wang or Songlin He.

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Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Communicated by Myung-Min Oh.

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Shang, W., Song, Y., Zhang, C. et al. Effects of light quality on growth, photosynthetic characteristics, and endogenous hormones in in vitro-cultured Lilium plantlets. Hortic. Environ. Biotechnol. 64, 65–81 (2023). https://doi.org/10.1007/s13580-022-00468-w

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  • DOI: https://doi.org/10.1007/s13580-022-00468-w

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