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Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

A laboratory-scale study was carried out to produce lignin peroxidase (ligninase) by white rot fungus (Phanerochaete chrysosporium) using sewage-treatment-plant (STP) sludge as the major substrate. The optimization was done using full-factorial design (FFD) with agitation and aeration as the two parameters. Nine experiments indicated by the FFD were fermented in a stirred-tank bioreactor for 3 days. A second-order quadratic model was developed using the regression analysis of the experimental results with the linear, quadratic, and interaction effects of the parameters. Analysis of variance (ANOVA) showed a high coefficient of determination (R 2) value of 0.972, thus indicating a satisfactory fit of the quadratic model with the experimental data. Using statistical analysis, the optimum aeration and agitation rates were determined to be 2.0 vvm and 200 rpm, respectively, with a maximum activity of 225 U l−1 in the first 3 days of fermentation. The validation experiment showed the maximum activity of lignin peroxidase was 744 U l−1 after 5 days of fermentation. The results for the tests of the stability of lignin peroxidase showed that the activity was more than 80% of the maximum for the first 12 h of incubation at an optimum pH of 5 and temperature of 55°C.

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Acknowledgements

The authors are grateful to the ISESCO-COMSTECH for their support in approving an International Research Grant and to the Department of Biotechnology Engineering, IIUM, for providing lab facilities to complete this work.

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Authors and Affiliations

  1. Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Gombak, 50728, Kuala Lumpur, Malaysia

    Md. Zahangir Alam & Mariatul F. Mansor

  2. Department of Biotechnology, Faculty of Science, International Islamic University, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Malaysia

    K. C. A. Jalal

  3. Bioenvironmental Engineering Research Unit (BERU), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia

    Md. Zahangir Alam

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  1. Md. Zahangir Alam
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  2. Mariatul F. Mansor
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Correspondence to Md. Zahangir Alam.

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Alam, M.Z., Mansor, M.F. & Jalal, K.C.A. Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor. J Ind Microbiol Biotechnol 36, 757–764 (2009). https://doi.org/10.1007/s10295-009-0548-5

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