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Ethanol production from banana peels at high pretreated substrate loading: comparison of two operational strategies

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Abstract

The potential of banana peels (BPs) as a source of fermentable sugars for ethanol production was evaluated using two yeasts: Saccharomyces cerevisiae commercial strain and Kluyveromyces marxianus thermotolerant strain isolated from Agave honey. Different process configurations: simultaneous saccharification and fermentation (SSF), as well as presaccharification and simultaneous saccharification and fermentation (PSSF) at high solid loading (up to 25% w/w), were studied. Similar ethanol production 13 g/L and 11 g/L was achieved at 10–12 h on SSF for S. cerevisiae and K. marxianus at 35 and 41 °C, respectively, with 10% (w/w) BP. The highest ethanol concentration (32.6 g/L) was reached on PSSF process (at 64 h) using commercial S. cerevisiae strain with 25% (w/v) BP, while ethanol production was inhibited in the case of K. marxianus under same assay conditions. BPs are a suitable source for second-generation ethanol production. The advantage of S. cerevisiae in the PSSF in comparison with K. marxianus was demonstrated. The present study demonstrated that BPs are the promising feedstock for ethanol production by PSSF.

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Abbreviations

BP:

Banana peels

SHF:

Separate hydrolysis and fermentation

SSF:

Simultaneous saccharification and fermentation

PSSF:

Presaccharification and simultaneous saccharification and fermentation

GC:

Gas chromatography

HPLC:

High-performance liquid chromatography

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Acknowledgments

The authors thank the Mexican National Council of Science and Technology (CONACYT) for the financial support at the project PDCPN 2013-01-213844, Ph.D. scholarship (No.560315), and the financial support under the program “Cátedras-CONACyT” (Project No. 729).

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Authors and Affiliations

  1. Department of Nanobioscience group and Food Research, Faculty of Chemistry Sciences, Autonomous, University of Coahuila, Blvd. V. Carranza e Ing. José Cárdenas Valdés, 25280, Saltillo, Coahuila, Mexico

    A. Sócrates Palacios, Anna Ilyina, Cristóbal N. Aguilar, José Luis Martínez-Hernández, Elda Patricia Segura-Ceniceros & Monica Lizeth Chávez González

  2. Escuela Superior Politécnica del Litoral, ESPOL, Facultad de Ingeniería en Mecánica y Ciencias de la Producción, ESPOL Polytechnic University Campus, Gustavo Galindo Km. 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador

    A. Sócrates Palacios

  3. CONACYT, Autonomous University of Coahuila, Blvd. V. Carranza e Ing.José Cárdenas Valdés, 25280, Saltillo, Coahuila, Mexico

    Rodolfo Ramos-González

  4. Research Center and Advanced Studies of IPN (CINVESTAV-IPN), 25903, Ramos Arizpe, Coahuila, Mexico

    Miguel Aguilar

  5. Biofuels Unit, CIEMAT Renewable Energy Division, 28040, Madrid, Spain

    Mercedes Ballesteros & José Miguel Oliva

  6. Department of Biorefinery Group, Food Research Faculty of Chemistry Sciences, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, Mexico

    Héctor A. Ruiz

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  1. A. Sócrates Palacios
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  2. Anna Ilyina
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  3. Rodolfo Ramos-González
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  4. Cristóbal N. Aguilar
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  5. José Luis Martínez-Hernández
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  6. Elda Patricia Segura-Ceniceros
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  7. Monica Lizeth Chávez González
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  8. Miguel Aguilar
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  9. Mercedes Ballesteros
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  10. José Miguel Oliva
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Correspondence to Anna Ilyina.

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Palacios, A.S., Ilyina, A., Ramos-González, R. et al. Ethanol production from banana peels at high pretreated substrate loading: comparison of two operational strategies. Biomass Conv. Bioref. 11, 1587–1596 (2021). https://doi.org/10.1007/s13399-019-00562-7

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