Abstract
Background
miRNAs play an important role in regulating normal animal development. Muscle tissue and fat metabolism are important for maintaining energy balance in animals. Yak has important agricultural and economic importance as it provides milk, meat, and hair. It is used for transportation as well. However, the miRNA expression profiles of their muscle and adipose tissue are currently unknown.
Objective
To explore the regulatory roles of miRNAs in the skeletal muscle and adipose tissues of yak.
Methods
A total of 12 small RNA libraries were constructed from the skeletal muscle and adipose samples from yak aged 0.5, 2.5, 4.5, and 7.5 years. High-throughput sequencing and bioinformatics analysis were used to determine the dynamic expression profile of miRNA, and a miRNA regulatory network related to muscle and adipose tissue development was established.
Results
miR-1-3p and miR-143-3p showed the highest expression during yak skeletal muscle and fat development, respectively. The MAPK and Ras signaling pathways were the pivotal pathways. miR-181-5p, miR-542-3p, and miR-424-5p may have key roles in skeletal muscle development, and CREBRF, GRB10, CDK1, RFX3, and EPC2 were the core target genes. While miR-127-5p, miR-379-3p, and miR-494-3p may play important regulatory roles in adipose deposition, and ETV1, XPO7, and C5AR2 were the core target genes.
Conclusion
This study provides valuable resources for further study of the molecular mechanisms underlying yak skeletal muscle and adipose tissue development, and also a basis for studying the interactions between genes and miRNAs.
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Funding
This work was supported by the National Beef and Yak Industry Technology System Project (CARS-37), Collaborative Innovation Center for Ecological Animal Husbandry of Qinghai-Tibetan Plateau Foundation (QZGYXT02), and the Tibet Autonomous Region Special Financial Project (XZNKY-2019-C-052).
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Ji, H., Wang, H., Ji, Q. et al. Differential expression profile of microRNA in yak skeletal muscle and adipose tissue during development. Genes Genom 42, 1347–1359 (2020). https://doi.org/10.1007/s13258-020-00988-8
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DOI: https://doi.org/10.1007/s13258-020-00988-8