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Pig farm environment as a source of beta-lactamase or AmpC-producing Klebsiella pneumoniae and Escherichia coli

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Abstract

The present study was undertaken to detect the occurrence of beta-lactamase-/AmpC-producing Klebsiella and Escherichia coli in healthy pigs, feed, drinking water, and pen floor or surface soil. The study also intended to detect the clonal relationship between the environmental and porcine isolates to confirm the route of transmission. Rectal swabs and environmental samples were collected from apparently healthy pigs kept in organized or backyard farms in India. The pigs had no history of antibiotic intake. Production of phenotypical beta-lactamase, associated genes, and class I integron gene was detected in E. coli and Klebsiella isolates. The phylogenetic relationship among the isolates was established on the basis of Random amplification of polymorphic DNA banding pattern. Beta-lactamase-producing Klebsiella were isolated from healthy pigs (20.0%), pen floor swabs/surface soil swabs (14.0%), and drinking water (100%). Escherichia coli isolated from healthy pigs (14.4%), pen floor/surface soil (8.0%), and drinking water (33.3%) were detected as beta-lactamase producers. Majority of beta-lactamase-producing isolates possessed blaCTX-M-9. Further, 35 (81%) Klebsiella and all the E. coli isolates were detected as AmpC beta-lactamase ACBL producers and possessed blaAmpC. Sixteen beta-lactamase-producing Klebsiella (37.20%) and 13 E. coli (86.67%) possessed class I integron. Few resistant isolates from environmental sources (surface soil swab and drinking water) and the studied pigs were detected within the same cluster of the dendrogram representing their similarities. The study indicated about the possible role of contaminated environment as a source of beta-lactamase/AmpC-producing Klebsiella and E. coli in pigs.

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Acknowledgements

The authors provide sincere thanks to honorable Vice Chancellor, West Bengal University of Animal and Fishery Sciences for the infrastructure and facilities. We also acknowledge the Director, Central Research Institute, Kasuli, HP, India for serogrouping of E. coli isolates.

Funding

The study was partially funded by Department of Biotechnology, Government of India (grant no.: BT/PR16149/NER/95/85/2015) and West Bengal State Council for Science and Technology (grant no.: 929(Sanc.)/ST/P/S and T/ 1G-25/2016).

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Authors and Affiliations

  1. Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, 37, K.B. Sarani, Belgachia, Kolkata, West Bengal, 700037, India

    Arindam Samanta, Achintya Mahanti, Souvick Chatterjee, Siddhartha Narayan Joardar & Indranil Samanta

  2. Eastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata, West Bengal, India

    Samiran Bandyopadhyay

  3. Department of Veterinary Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences, 37, K.B. Sarani, Belgachia, Kolkata, West Bengal, 700037, India

    Tapas Kumar Sar

  4. Department of Animal Nutrition, West Bengal University of Animal and Fishery Sciences, 37, K.B. Sarani, Belgachia, Kolkata, West Bengal, 700037, India

    Guru Prasad Mandal

  5. Department of Veterinary Microbiology, Central Agricultural University, Aizawl, Mizoram, India

    Tapan Kumar Dutta

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  1. Arindam Samanta
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  9. Indranil Samanta
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Samanta, A., Mahanti, A., Chatterjee, S. et al. Pig farm environment as a source of beta-lactamase or AmpC-producing Klebsiella pneumoniae and Escherichia coli. Ann Microbiol 68, 781–791 (2018). https://doi.org/10.1007/s13213-018-1387-2

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