Abstract
The Aspergillus fumigatus CAS21 tannase was spray dried with β-cyclodextrin, Capsul® starch, soybean meal, lactose, and maltodextrin as adjuvants. The moisture content and water activity of the products ranged from 5.6 to 11.5% and from 0.249 to 0.448, respectively. The maximal tannase activity was achieved at 40–60 ºC and pH 5.0–6.0 for the powders containing β-cyclodextrin and Capsul® starch, which was stable at 40 ºC and 40–60 ºC for 120 min, respectively. For all the dried products, tannase retained its activity of over 80% for 120 min at pH 5.0 and 6.0. Salts and solvents influenced the activity of the spray-dried tannase. The activity of the spray-dried tannase was maintained when preserved for 1 year at 4 ºC and 28 ºC. Spray-dried tannase reduced the content of tannins and polyphenolic compounds of leather effluent and sorghum flour and catalyzed the transesterification reaction. The spray drying process stabilized the tannase activity, highlighting the potential of dried products for biotechnological applications.
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Acknowledgements
The authors gratefully acknowledge the financial support from Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP (Process no 2016/11311-5) and Ministério da Ciência, Tecnologia, Inovações e Comunicações (CNPq) (142389/2017-0). They also thank Maurício de Oliveira for the technical assistance and Laboratório de P&D em Processos Farmacêuticos e Biotecnológicos from Universidade de São Paulo (LAPROFAR/USP) for development of the drying tests and Carlos Ambrosio from ARTEZA, who provided the effluent samples. The manuscript is a part of the doctoral thesis of R.M.V.F., who is financed by Ministério da Ciência, Tecnologia, Inovações e Comunicações (CNPq).
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RMFC performed the laboratory work, studied, and prepared the MS; MLLM supported the spray-drying assays; WPO planned and supervised the spray-drying assays and prepared the MS, and LHSG planned and supervised the whole work, and prepared the manuscript.
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Cavalcanti, R.M.F., Martinez, M.L.L., Oliveira, W.P. et al. Stabilization and application of spray-dried tannase from Aspergillus fumigatus CAS21 in the presence of different carriers. 3 Biotech 10, 177 (2020). https://doi.org/10.1007/s13205-020-2164-z
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DOI: https://doi.org/10.1007/s13205-020-2164-z