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Degradation of acetochlor and beneficial effect of phosphate-solubilizing Bacillus sp. ACD-9 on maize seedlings

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Abstract

The biodegradation of acetochlor in solution and soil and improvements in the growth of maize seedlings by a phosphate-solubilizing bacterial strain were investigated in this research. The strain Bacillus sp. ACD-9 optimally degraded acetochlor at pH 6.0 and 42 °C in solution. And acetochlor with an initial concentration of 30 mg/L was efficiently (> 60%) degraded by the strain after 2 days in solution. Acetochlor biodegradation and the resulting beneficial products were also identified by LC–MS, and the probable degradation products of acetochlor and two kinds of plant growth hormones, namely, 2-chloro-N-(2-methyl-6-ethylphenyl) acetamide (CMEPA), indoleacetic acid (IAA), and zeatin, were detected from the fermentation broth of strain ACD-9. The effects of the strain on the growth and acetochlor accumulation of maize seedlings were also analyzed in laboratory-scale pot experiments. Inoculation of the strain in soil could significantly improve growth (> 9.4%) and phosphorus uptake (> 14.8%) and decrease the accumulation (> 70%) and toxic effects of acetochlor on seedlings. Taking the results together, strain ACD-9 may be useful in the degradation of acetochlor in soil and promotion of the growth and phosphorus uptake of maize.

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Acknowledgements

This research was supported by the projects of the National Natural Science Foundation of China (No. 31400103 and No. 31370147) and Young Key Teachers Training Program of Henan University of Technology.

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Authors and Affiliations

  1. College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, Henan, China

    Haifeng Li, Yuanli Wang, Jiake Fu, Shuang Hu & Jianhang Qu

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  1. Haifeng Li
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  2. Yuanli Wang
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  3. Jiake Fu
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  4. Shuang Hu
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  5. Jianhang Qu
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Correspondence to Haifeng Li.

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Li, H., Wang, Y., Fu, J. et al. Degradation of acetochlor and beneficial effect of phosphate-solubilizing Bacillus sp. ACD-9 on maize seedlings. 3 Biotech 10, 67 (2020). https://doi.org/10.1007/s13205-020-2056-2

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