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Optimization of fed-batch fermentation and direct spray drying in the preparation of microbial inoculant of acetochlor-degrading strain Sphingomonas sp. DC-6

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Abstract

Microbial inoculant preparation is a prerequisite for its application in large-scale bioremediation. In the present study, Sphingomonas sp. DC-6, an efficient acetochlor-degrading strain, was used to investigate the process of preparing the inoculant. Optimization of submerged fermentation (SmF) by response surface methodology (RSM) resulted in a first 22% increase in biomass of liquid inoculant. Then, a biomass increase of 2.18 times with 14.58% shortened incubation period was further obtained in optimized medium using a 7.5-l bioreactor. However, less than 0.4% viable cells in liquid inoculant survived after 180-days storage. Thus, optimized spray drying conditions were subsequently employed for the production of high viability powder (2.11 × 1012 cfu g− 1 powder) without additive and its survival ratio (SR) after 180-days storage was still maintained at 90.5%. Both the 180-days stored powder and the original powder showed the same degradation performance, being able to completely degrade 200 mg l− 1 acetochlor within 48 h. This study demonstrated that strain DC-6 was suitable for industrial production of bacteria powder and provided a potential approach for the preparation of pesticide-degrading microbial inoculant.

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Acknowledgements

This work was supported by the National Key R&D Program of China (2017YFD0800702), the National Natural Science Foundation of China (31670112 and 31500041), the Fundamental Reserach Funds for the Central Universities (KYZ201861 and KJQN201645) and the Jiangsu Agriculture Science and Technology Innovation Fund CX (15) 1004.

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Authors and Affiliations

  1. Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Hui Wang, Kun Jiang, Ziwei Zhu, Wankui Jiang, Zhangong Yang, Shijun Zhu, Jiguo Qiu, Xin Yan, Jian He, Qin He & Qing Hong

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  1. Hui Wang
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  2. Kun Jiang
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  3. Ziwei Zhu
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  4. Wankui Jiang
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  6. Shijun Zhu
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  9. Jian He
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  10. Qin He
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  11. Qing Hong
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Correspondence to Qin He.

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Wang, H., Jiang, K., Zhu, Z. et al. Optimization of fed-batch fermentation and direct spray drying in the preparation of microbial inoculant of acetochlor-degrading strain Sphingomonas sp. DC-6. 3 Biotech 8, 294 (2018). https://doi.org/10.1007/s13205-018-1324-x

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