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Biofilm formation in marine bacteria and biocidal sensitivity: interplay between a potent antibiofilm compound (AS162) and quorum-sensing autoinducers

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Abstract

The capacity of two homoserine lactones to stimulate the marine bacteria Pseudoalteromonas ulvae (TC14 strain) for its capacity to form a biofilm when exposed to a potent antibiofilm compound AS162 is reported. Effective concentrations (EC50) of AS162 at 24 h, 48 h, and 72 h were, respectively, of 4.3, 4.4, and 6.0 µM. When tested in combination with HSLs, results showed that quorum-sensing signal molecules 3-oxo-C6 and 3-oxo-C8 homoserine lactones do not act directly on the biofilm formation, but are able to interfere positively with AS162 to promote biofilm growth with EC50 ranging from 30 to 50 µM. The same results were obtained with two other marine bacterial strains: Pseudoalteromonas lipolytica TC8 and Paracoccus sp. 4M6. These findings suggest that HSLs can significantly affect the biocidal sensitivity of marine bacteria to antifouling agents.

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Acknowledgements

This research did not receive any specific grant from funding agencies (public, or private). The Paracoccus sp. strain 4M6 was provided by the LBCM (Université de Bretagne Sud).

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Authors and Affiliations

  1. Université de Toulon, MAPIEM, Toulon, France

    Emmanuel Gozoua & Yves Blache

  2. Laboratoire de Microbiologie et Biotechnologie, Université Nangui-Abrogoua, Abidjan, Côte d’Ivoire

    Emmanuel Gozoua & Rose Koffi-Nevry

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  1. Emmanuel Gozoua
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  2. Rose Koffi-Nevry
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  3. Yves Blache
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Correspondence to Yves Blache.

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Gozoua, E., Koffi-Nevry, R. & Blache, Y. Biofilm formation in marine bacteria and biocidal sensitivity: interplay between a potent antibiofilm compound (AS162) and quorum-sensing autoinducers. 3 Biotech 9, 338 (2019). https://doi.org/10.1007/s13205-019-1866-6

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