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Studies on a better laccase-producing mutant of Fusarium incarnatum LD-3 under solid substrate tray fermentation

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Abstract

Forty-seven (47) mutant strains were generated from the wild-type fungus, Fusarium incarnatum strain LD-3 after exposure to ultraviolet radiation (UV) and a further seventeen (17) mutants were generated after exposure to ethyl methane sulfonate (EMS). Amongst these, the mutant strain, identified as UC-14, was the most promising laccase producer and produced threefold more laccase than the wild strain LD-3. Solid substrate tray fermentation using wheat straw and rice bran showed a twofold increase in laccase productivity and a fivefold loss of total organic matter (TOM) by mutant UC-14 over the wild strain LD-3. The mutant strain UC-14 also showed 25% and 54% weight loss of TOM after 36 days of fermentation which was 10% higher than the wild-type LD-3. Scanning electron microscopy suggested that the delayed condidiation in mutant strain UC-14 may be responsible for better laccase production.

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Acknowledgements

Financial assistance provided by University Grants Commission (UGC), New Delhi to carry out the aforementioned research work is acknowledged by the authors. (Sanction no: F.47–052/06.)

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  1. Department of Microbiology, Natubhai V. Patel College of Pure and Applied Sciences, Sardar Patel University, Near Mota Bazar Post Office, Mota Bazar, Vallabh Vidyanagar, Gujarat, 388120, India

    Urvish Chhaya & Akshaya Gupte

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  1. Urvish Chhaya
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  2. Akshaya Gupte
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Correspondence to Akshaya Gupte.

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Chhaya, U., Gupte, A. Studies on a better laccase-producing mutant of Fusarium incarnatum LD-3 under solid substrate tray fermentation. 3 Biotech 9, 100 (2019). https://doi.org/10.1007/s13205-019-1605-z

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