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Pectinolytic enzymes-solid state fermentation, assay methods and applications in fruit juice industries: a review

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Abstract

A plethora of solid substrates, cultivation conditions and enzyme assay methods have been used for efficient production and estimation of polygalacturonase and pectin methylesterase enzymes. Recent developments in industrial biotechnology offer several opportunities for the utilization of low cost agro-industrial waste in Solid State Fermentation (SSF) for the pectinolytic enzyme production using fungi. Fruit waste mainly citrus fruit waste alone and along with other agro-industrial waste has been explored in SSF for enzyme production. Agro-industrial waste, due to the economic advantage of low procuring cost has been employed in SSF bioreactors for pectinolytic enzyme production. Acidic pectinases produced by fungi are utilized especially in food industries for clarification of fruit juices. This review focuses on the recent developments in SSF processes utilizing agro-industrial residues for polygalacturonase and pectin methylesterase production, their various assay methods and applications in fruit juice industries.

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Fig. 1
Fig. 2
Fig. 3
Fig. 4

Modified from Ruiz et al. (2012)

Fig. 5

Modified from Diaz et al. (2013)

Fig. 6

Modified from Rodríguez-Fernández et al. (2011)

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Acknowledgements

The authors acknowledge the facilities provided by the Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi (DBT) in the School of Biotechnology, Devi Ahilya University, Indore under M.Sc. Biotechnology Program and Bioinformatics sub center. The authors also acknowledge facilities of Department of Biosciences, Maharaja Ranjit Singh College of Professional Sciences, Indore.

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  1. Maharaja Ranjit Singh College of Professional Sciences, Hemkunt Campus, Khandwa Road, Indore, 452001, India

    Mukesh Kumar Patidar & Anand Nighojkar

  2. Mata Gujri College of Professional Studies, A.B. Road, Indore, 452001, India

    Sadhana Nighojkar

  3. School of Biotechnology, Devi Ahilya University, Khandwa Road, Indore, 452001, India

    Anil Kumar

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Patidar, M.K., Nighojkar, S., Kumar, A. et al. Pectinolytic enzymes-solid state fermentation, assay methods and applications in fruit juice industries: a review. 3 Biotech 8, 199 (2018). https://doi.org/10.1007/s13205-018-1220-4

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